Hepatic parenchymal changes following transcatheter embolization and chemoembolization in a rabbit tumor model.
OBJECTIVE: To compare the effects of transcatheter arterial chemoembolization (TACE) with transcatheter arterial embolization (TAE) on liver function, hepatic damage, and hepatic fibrogenesis in a rabbit tumor model. MATERIALS AND METHODS: Thirty-nine New Zealand white rabbits implanted with VX2 tum...
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doaj-c1cc6b4b461047c3bd77c9e6940a7c652020-11-25T01:32:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7075710.1371/journal.pone.0070757Hepatic parenchymal changes following transcatheter embolization and chemoembolization in a rabbit tumor model.Yong WangBin XiongBin LiangHui ZhaoHui LiJun QianHui-Min LiangGan-Sheng FengChuan-Sheng ZhengOBJECTIVE: To compare the effects of transcatheter arterial chemoembolization (TACE) with transcatheter arterial embolization (TAE) on liver function, hepatic damage, and hepatic fibrogenesis in a rabbit tumor model. MATERIALS AND METHODS: Thirty-nine New Zealand white rabbits implanted with VX2 tumors in the left liver lobes were randomly divided into three groups: TAE, TACE, and control group. In the TAE group (n = 15), polyvinyl alcohol particles (PVAs) were used for left hepatic artery embolization. In the TACE group (n = 15), the tumors were treated with left hepatic arterial infusions of a suspension of 10-hydroxycamptothecin and lipiodol, followed by embolization with PVAs. In the control group (n = 9), the animals received sham treatment with distilled water. Serum and liver samples were collected at 6 hours, 3 days and 7 days after treatment. Liver damage was measured using a liver function test and histological analyses. Liver fibrogenesis and hepatic stellate cell (HSC) activation were evaluated using Sirius Red and anti-alpha-smooth muscle actin (α-SMA) immunohistochemical stains. RESULTS: TACE caused liver injury with greater increases in serum alanine aminotransferase and aspartate aminotransferase levels on day 3 (P<0.05). Histological analyses revealed increased hepatic necrosis in adjacent non-tumorous liver tissue from day 3 compared to the TAE group (Suzuki score of 2.33±1.29 versus 1.13±1.18, P = 0.001). HSC activation and proliferation were significantly increased in the TACE group compared to the control group at 3 and 7 days after treatment (0.074±0.014 vs. 0.010±0.006, and 0.088±0.023 vs. 0.017±0.009, P<0.05). Sirius Red staining demonstrated a statistically significant increase in collagen deposition in the livers in the TACE group 7 days after embolization compared to the control group (0.118±0.012 vs. 0.060±0.017, P = 0.05). CONCLUSION: The results of this animal study revealed that TACE induced prominent hepatocellular damage and hepatic fibrogenesis, which compromised liver function and may be responsible for chronic liver decompensation.http://europepmc.org/articles/PMC3743795?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong Wang Bin Xiong Bin Liang Hui Zhao Hui Li Jun Qian Hui-Min Liang Gan-Sheng Feng Chuan-Sheng Zheng |
spellingShingle |
Yong Wang Bin Xiong Bin Liang Hui Zhao Hui Li Jun Qian Hui-Min Liang Gan-Sheng Feng Chuan-Sheng Zheng Hepatic parenchymal changes following transcatheter embolization and chemoembolization in a rabbit tumor model. PLoS ONE |
author_facet |
Yong Wang Bin Xiong Bin Liang Hui Zhao Hui Li Jun Qian Hui-Min Liang Gan-Sheng Feng Chuan-Sheng Zheng |
author_sort |
Yong Wang |
title |
Hepatic parenchymal changes following transcatheter embolization and chemoembolization in a rabbit tumor model. |
title_short |
Hepatic parenchymal changes following transcatheter embolization and chemoembolization in a rabbit tumor model. |
title_full |
Hepatic parenchymal changes following transcatheter embolization and chemoembolization in a rabbit tumor model. |
title_fullStr |
Hepatic parenchymal changes following transcatheter embolization and chemoembolization in a rabbit tumor model. |
title_full_unstemmed |
Hepatic parenchymal changes following transcatheter embolization and chemoembolization in a rabbit tumor model. |
title_sort |
hepatic parenchymal changes following transcatheter embolization and chemoembolization in a rabbit tumor model. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
OBJECTIVE: To compare the effects of transcatheter arterial chemoembolization (TACE) with transcatheter arterial embolization (TAE) on liver function, hepatic damage, and hepatic fibrogenesis in a rabbit tumor model. MATERIALS AND METHODS: Thirty-nine New Zealand white rabbits implanted with VX2 tumors in the left liver lobes were randomly divided into three groups: TAE, TACE, and control group. In the TAE group (n = 15), polyvinyl alcohol particles (PVAs) were used for left hepatic artery embolization. In the TACE group (n = 15), the tumors were treated with left hepatic arterial infusions of a suspension of 10-hydroxycamptothecin and lipiodol, followed by embolization with PVAs. In the control group (n = 9), the animals received sham treatment with distilled water. Serum and liver samples were collected at 6 hours, 3 days and 7 days after treatment. Liver damage was measured using a liver function test and histological analyses. Liver fibrogenesis and hepatic stellate cell (HSC) activation were evaluated using Sirius Red and anti-alpha-smooth muscle actin (α-SMA) immunohistochemical stains. RESULTS: TACE caused liver injury with greater increases in serum alanine aminotransferase and aspartate aminotransferase levels on day 3 (P<0.05). Histological analyses revealed increased hepatic necrosis in adjacent non-tumorous liver tissue from day 3 compared to the TAE group (Suzuki score of 2.33±1.29 versus 1.13±1.18, P = 0.001). HSC activation and proliferation were significantly increased in the TACE group compared to the control group at 3 and 7 days after treatment (0.074±0.014 vs. 0.010±0.006, and 0.088±0.023 vs. 0.017±0.009, P<0.05). Sirius Red staining demonstrated a statistically significant increase in collagen deposition in the livers in the TACE group 7 days after embolization compared to the control group (0.118±0.012 vs. 0.060±0.017, P = 0.05). CONCLUSION: The results of this animal study revealed that TACE induced prominent hepatocellular damage and hepatic fibrogenesis, which compromised liver function and may be responsible for chronic liver decompensation. |
url |
http://europepmc.org/articles/PMC3743795?pdf=render |
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