Analysis of CPD ultraviolet lesion bypass in chicken DT40 cells: polymerase η and PCNA ubiquitylation play identical roles.
Translesion synthesis (TLS) provides a mechanism of copying damaged templates during DNA replication. This potentially mutagenic process may operate either at the replication fork or at post-replicative gaps. We used the example of T-T cyclobutane pyrimidine dimer (CPD) bypass to determine the influ...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3525536?pdf=render |
id |
doaj-c1d45c4373cd4473b07f306a92333905 |
---|---|
record_format |
Article |
spelling |
doaj-c1d45c4373cd4473b07f306a923339052020-11-25T02:16:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5247210.1371/journal.pone.0052472Analysis of CPD ultraviolet lesion bypass in chicken DT40 cells: polymerase η and PCNA ubiquitylation play identical roles.Agnes VargaAdam P MarcusMasayuki HimotoShigenori IwaiDávid SzütsTranslesion synthesis (TLS) provides a mechanism of copying damaged templates during DNA replication. This potentially mutagenic process may operate either at the replication fork or at post-replicative gaps. We used the example of T-T cyclobutane pyrimidine dimer (CPD) bypass to determine the influence of polymerase recruitment via PCNA ubiquitylation versus the REV1 protein on the efficiency and mutagenic outcome of TLS. Using mutant chicken DT40 cell lines we show that, on this numerically most important UV lesion, defects in polymerase η or in PCNA ubiquitylation similarly result in the long-term failure of lesion bypass with persistent strand gaps opposite the lesion, and the elevation of mutations amongst successful TLS events. Our data suggest that PCNA ubiquitylation promotes CPD bypass mainly by recruiting polymerase η, resulting in the majority of CPD lesions bypassed in an error-free manner. In contrast, we find that polymerase ζ is responsible for the majority of CPD-dependent mutations, but has no essential function in the completion of bypass. These findings point to a hierarchy of access of the different TLS polymerases to the lesion, suggesting a temporal order of their recruitment. The similarity of REV1 and REV3 mutant phenotypes confirms that the involvement of polymerase ζ in TLS is largely determined by its recruitment to DNA by REV1. Our data demonstrate the influence of the TLS polymerase recruitment mechanism on the success and accuracy of bypass.http://europepmc.org/articles/PMC3525536?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Agnes Varga Adam P Marcus Masayuki Himoto Shigenori Iwai Dávid Szüts |
spellingShingle |
Agnes Varga Adam P Marcus Masayuki Himoto Shigenori Iwai Dávid Szüts Analysis of CPD ultraviolet lesion bypass in chicken DT40 cells: polymerase η and PCNA ubiquitylation play identical roles. PLoS ONE |
author_facet |
Agnes Varga Adam P Marcus Masayuki Himoto Shigenori Iwai Dávid Szüts |
author_sort |
Agnes Varga |
title |
Analysis of CPD ultraviolet lesion bypass in chicken DT40 cells: polymerase η and PCNA ubiquitylation play identical roles. |
title_short |
Analysis of CPD ultraviolet lesion bypass in chicken DT40 cells: polymerase η and PCNA ubiquitylation play identical roles. |
title_full |
Analysis of CPD ultraviolet lesion bypass in chicken DT40 cells: polymerase η and PCNA ubiquitylation play identical roles. |
title_fullStr |
Analysis of CPD ultraviolet lesion bypass in chicken DT40 cells: polymerase η and PCNA ubiquitylation play identical roles. |
title_full_unstemmed |
Analysis of CPD ultraviolet lesion bypass in chicken DT40 cells: polymerase η and PCNA ubiquitylation play identical roles. |
title_sort |
analysis of cpd ultraviolet lesion bypass in chicken dt40 cells: polymerase η and pcna ubiquitylation play identical roles. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Translesion synthesis (TLS) provides a mechanism of copying damaged templates during DNA replication. This potentially mutagenic process may operate either at the replication fork or at post-replicative gaps. We used the example of T-T cyclobutane pyrimidine dimer (CPD) bypass to determine the influence of polymerase recruitment via PCNA ubiquitylation versus the REV1 protein on the efficiency and mutagenic outcome of TLS. Using mutant chicken DT40 cell lines we show that, on this numerically most important UV lesion, defects in polymerase η or in PCNA ubiquitylation similarly result in the long-term failure of lesion bypass with persistent strand gaps opposite the lesion, and the elevation of mutations amongst successful TLS events. Our data suggest that PCNA ubiquitylation promotes CPD bypass mainly by recruiting polymerase η, resulting in the majority of CPD lesions bypassed in an error-free manner. In contrast, we find that polymerase ζ is responsible for the majority of CPD-dependent mutations, but has no essential function in the completion of bypass. These findings point to a hierarchy of access of the different TLS polymerases to the lesion, suggesting a temporal order of their recruitment. The similarity of REV1 and REV3 mutant phenotypes confirms that the involvement of polymerase ζ in TLS is largely determined by its recruitment to DNA by REV1. Our data demonstrate the influence of the TLS polymerase recruitment mechanism on the success and accuracy of bypass. |
url |
http://europepmc.org/articles/PMC3525536?pdf=render |
work_keys_str_mv |
AT agnesvarga analysisofcpdultravioletlesionbypassinchickendt40cellspolymeraseēandpcnaubiquitylationplayidenticalroles AT adampmarcus analysisofcpdultravioletlesionbypassinchickendt40cellspolymeraseēandpcnaubiquitylationplayidenticalroles AT masayukihimoto analysisofcpdultravioletlesionbypassinchickendt40cellspolymeraseēandpcnaubiquitylationplayidenticalroles AT shigenoriiwai analysisofcpdultravioletlesionbypassinchickendt40cellspolymeraseēandpcnaubiquitylationplayidenticalroles AT davidszuts analysisofcpdultravioletlesionbypassinchickendt40cellspolymeraseēandpcnaubiquitylationplayidenticalroles |
_version_ |
1724893420787335168 |