Fluoride Modification of Titanium Surfaces Enhance Complement Activation

Immediately after dental implant insertion, blood will be in direct contact and interact with the implant surface and activates inflammatory responses and complement cascades within seconds. The aim of the present study was to determine the ability of fluoride-modified titanium surfaces to activate...

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Main Authors: Maria H. Pham, Håvard J. Haugen, Janne E. Reseland
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Materials
Subjects:
Online Access:https://www.mdpi.com/1996-1944/13/3/684
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spelling doaj-c1d4f36b5edc4fdf85ae2c1f60fdd4c62020-11-25T02:16:08ZengMDPI AGMaterials1996-19442020-02-0113368410.3390/ma13030684ma13030684Fluoride Modification of Titanium Surfaces Enhance Complement ActivationMaria H. Pham0Håvard J. Haugen1Janne E. Reseland2Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 0317 Oslo, NorwayDepartment of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 0317 Oslo, NorwayDepartment of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 0317 Oslo, NorwayImmediately after dental implant insertion, blood will be in direct contact and interact with the implant surface and activates inflammatory responses and complement cascades within seconds. The aim of the present study was to determine the ability of fluoride-modified titanium surfaces to activate complement cascades using the human buffy coat as model. The buffy coats were exposed to hydrofluoric acid-modified surfaces for a short time and its responses were compared to controls. Identification and quantification of complement cascade biomarkers were conducted using ELISA kits and multianalyte profiling using Luminex. A lower level of C3 at 30 min and increased levels of C4, MIP-4, CRP, and pigment epithelium-derived factor at 360 min were found on modified surfaces as compared to controls. We found no significant differences in the levels of C3a, C5a, C Factor H, α2M, ApoA1, ApoC3, ApoE, Prealbumin, α1AT, and SAP in modified surfaces in the buffy coats. We conclude that titanium surfaces treated with hydrofluoric acid modify the levels of specific biomarkers related to the complement cascade and angiogenesis and, thus, tissue growth, remodeling and repair, as this may play a role in the enhanced clinical performance of fluoride-modified Ti dental implants.https://www.mdpi.com/1996-1944/13/3/684biomaterialstitaniumhydrofluoric aciddental implantsbuffy coatacute reactionsurface modification
collection DOAJ
language English
format Article
sources DOAJ
author Maria H. Pham
Håvard J. Haugen
Janne E. Reseland
spellingShingle Maria H. Pham
Håvard J. Haugen
Janne E. Reseland
Fluoride Modification of Titanium Surfaces Enhance Complement Activation
Materials
biomaterials
titanium
hydrofluoric acid
dental implants
buffy coat
acute reaction
surface modification
author_facet Maria H. Pham
Håvard J. Haugen
Janne E. Reseland
author_sort Maria H. Pham
title Fluoride Modification of Titanium Surfaces Enhance Complement Activation
title_short Fluoride Modification of Titanium Surfaces Enhance Complement Activation
title_full Fluoride Modification of Titanium Surfaces Enhance Complement Activation
title_fullStr Fluoride Modification of Titanium Surfaces Enhance Complement Activation
title_full_unstemmed Fluoride Modification of Titanium Surfaces Enhance Complement Activation
title_sort fluoride modification of titanium surfaces enhance complement activation
publisher MDPI AG
series Materials
issn 1996-1944
publishDate 2020-02-01
description Immediately after dental implant insertion, blood will be in direct contact and interact with the implant surface and activates inflammatory responses and complement cascades within seconds. The aim of the present study was to determine the ability of fluoride-modified titanium surfaces to activate complement cascades using the human buffy coat as model. The buffy coats were exposed to hydrofluoric acid-modified surfaces for a short time and its responses were compared to controls. Identification and quantification of complement cascade biomarkers were conducted using ELISA kits and multianalyte profiling using Luminex. A lower level of C3 at 30 min and increased levels of C4, MIP-4, CRP, and pigment epithelium-derived factor at 360 min were found on modified surfaces as compared to controls. We found no significant differences in the levels of C3a, C5a, C Factor H, α2M, ApoA1, ApoC3, ApoE, Prealbumin, α1AT, and SAP in modified surfaces in the buffy coats. We conclude that titanium surfaces treated with hydrofluoric acid modify the levels of specific biomarkers related to the complement cascade and angiogenesis and, thus, tissue growth, remodeling and repair, as this may play a role in the enhanced clinical performance of fluoride-modified Ti dental implants.
topic biomaterials
titanium
hydrofluoric acid
dental implants
buffy coat
acute reaction
surface modification
url https://www.mdpi.com/1996-1944/13/3/684
work_keys_str_mv AT mariahpham fluoridemodificationoftitaniumsurfacesenhancecomplementactivation
AT havardjhaugen fluoridemodificationoftitaniumsurfacesenhancecomplementactivation
AT janneereseland fluoridemodificationoftitaniumsurfacesenhancecomplementactivation
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