Chemical composition and pharmacological activities of Pisum sativum
Abstract Background Consumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore th...
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doaj-c1e239966afa43b1b3c707b5c7b9e6eb2020-11-25T04:01:41ZengBMCBMC Complementary and Alternative Medicine1472-68822017-03-011711910.1186/s12906-017-1699-yChemical composition and pharmacological activities of Pisum sativumMd Nazmul Hasan Zilani0Tamanna Sultana1S. M. Asabur Rahman2Md Anisuzzman3Md Amirul Islam4Jamil A. Shilpi5Md Golam Hossain6Pharmacy Discipline, Life Science School, Khulna UniversityPharmacy Discipline, Life Science School, Khulna UniversityPharmacy Discipline, Life Science School, Khulna UniversityPharmacy Discipline, Life Science School, Khulna UniversityPharmacy Discipline, Life Science School, Khulna UniversityPharmacy Discipline, Life Science School, Khulna UniversityPharmacy Discipline, Life Science School, Khulna UniversityAbstract Background Consumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore the traditional use of aerial parts of P. sativum as a source of antidiabetic agent. In addition, antioxidant activity and chemical composition was carried out. Methods Total polyphenol content was spectrophotometrically determined using Folin Chiocalteu’s reagent while the flavonoids by aluminum chloride colorimetric assay. Identification of compounds of the extract was made through HPLC and LCMS. Antihyperglycemic activity was assessed by oral glucose tolerance test in mice. Antioxidant activity was determined by DPPH free radical scavenging and reducing power assay. Results Total polyphenol and total flavonoids content were found to be 51.23 mg gallic acid equivalent and 30.88 mg quercetin equivalent per gram of dried plant extract respectively. Ellagic acid and p-coumeric acid were detected through HPLC. A total of eight compounds including naringenin, β-sitosterol were indentified through LCMS. In OGTT, extract (200 mg/kg bw) showed a 30.24% decrease (P< 0.05) in blood glucose levels at 30 min compared to the normal control. The extract showed IC50 value of 158.52 μg/mL in DPPH scavenging assay and also showed comparable reducing power. Conclusion Along with other compounds ellagic acid and β-sitosterol present in the extract may be responsible for its antioxidant as well as antihyperglycemic activities. Altogether these results rationalize the use of this vegetable in traditional medicine.http://link.springer.com/article/10.1186/s12906-017-1699-yHPLCLCMSEllagic acidβ-sitosterolAntioxidantAntidiabetic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Md Nazmul Hasan Zilani Tamanna Sultana S. M. Asabur Rahman Md Anisuzzman Md Amirul Islam Jamil A. Shilpi Md Golam Hossain |
spellingShingle |
Md Nazmul Hasan Zilani Tamanna Sultana S. M. Asabur Rahman Md Anisuzzman Md Amirul Islam Jamil A. Shilpi Md Golam Hossain Chemical composition and pharmacological activities of Pisum sativum BMC Complementary and Alternative Medicine HPLC LCMS Ellagic acid β-sitosterol Antioxidant Antidiabetic |
author_facet |
Md Nazmul Hasan Zilani Tamanna Sultana S. M. Asabur Rahman Md Anisuzzman Md Amirul Islam Jamil A. Shilpi Md Golam Hossain |
author_sort |
Md Nazmul Hasan Zilani |
title |
Chemical composition and pharmacological activities of Pisum sativum |
title_short |
Chemical composition and pharmacological activities of Pisum sativum |
title_full |
Chemical composition and pharmacological activities of Pisum sativum |
title_fullStr |
Chemical composition and pharmacological activities of Pisum sativum |
title_full_unstemmed |
Chemical composition and pharmacological activities of Pisum sativum |
title_sort |
chemical composition and pharmacological activities of pisum sativum |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2017-03-01 |
description |
Abstract Background Consumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore the traditional use of aerial parts of P. sativum as a source of antidiabetic agent. In addition, antioxidant activity and chemical composition was carried out. Methods Total polyphenol content was spectrophotometrically determined using Folin Chiocalteu’s reagent while the flavonoids by aluminum chloride colorimetric assay. Identification of compounds of the extract was made through HPLC and LCMS. Antihyperglycemic activity was assessed by oral glucose tolerance test in mice. Antioxidant activity was determined by DPPH free radical scavenging and reducing power assay. Results Total polyphenol and total flavonoids content were found to be 51.23 mg gallic acid equivalent and 30.88 mg quercetin equivalent per gram of dried plant extract respectively. Ellagic acid and p-coumeric acid were detected through HPLC. A total of eight compounds including naringenin, β-sitosterol were indentified through LCMS. In OGTT, extract (200 mg/kg bw) showed a 30.24% decrease (P< 0.05) in blood glucose levels at 30 min compared to the normal control. The extract showed IC50 value of 158.52 μg/mL in DPPH scavenging assay and also showed comparable reducing power. Conclusion Along with other compounds ellagic acid and β-sitosterol present in the extract may be responsible for its antioxidant as well as antihyperglycemic activities. Altogether these results rationalize the use of this vegetable in traditional medicine. |
topic |
HPLC LCMS Ellagic acid β-sitosterol Antioxidant Antidiabetic |
url |
http://link.springer.com/article/10.1186/s12906-017-1699-y |
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