<i>Bacillus telluris</i> sp. nov. Isolated from Greenhouse Soil in Beijing, China
A novel Gram-stain-positive, rod-shaped, endospore-forming bacterium, which we designated as strain 03113<sup>T</sup>, was isolated from greenhouse soil in Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed strain 03113<sup>T</sup> is in the genus &...
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doaj-c1e376205a4c4c159144f0a5e94a93612020-11-25T02:41:49ZengMDPI AGMicroorganisms2076-26072020-05-01870270210.3390/microorganisms8050702<i>Bacillus telluris</i> sp. nov. Isolated from Greenhouse Soil in Beijing, ChinaHe-Bao Guo0Shan-Wen He1Xing Wang2Kyu-Kyu Thin3Hai-Lei Wei4Xiao-Xia Zhang5Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; <email>guohebao0409@163.com</email> (H.-B.G.)Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; <email>guohebao0409@163.com</email> (H.-B.G.)Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; <email>guohebao0409@163.com</email> (H.-B.G.)Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; <email>guohebao0409@163.com</email> (H.-B.G.)Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; <email>guohebao0409@163.com</email> (H.-B.G.)Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; <email>guohebao0409@163.com</email> (H.-B.G.)A novel Gram-stain-positive, rod-shaped, endospore-forming bacterium, which we designated as strain 03113<sup>T</sup>, was isolated from greenhouse soil in Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed strain 03113<sup>T</sup> is in the genus <i>Bacillus</i> and had the highest similarity to <i>Bacillus solani</i> CCTCC AB 2014277<sup>T</sup> (98.14%). The strain grew at 4 °C–50 °C (optimum 37 °C), with 0–10% (<i>w/v</i>) NaCl (optimum 5%), and in the range of pH 3.0–12.0 (optimum pH 8.0). Menaquinone was identified as MK-7, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The main major cellular fatty acids detected were anteiso-C<sub>15:0</sub> (51.35%) and iso-C<sub>15:0</sub> (11.06%), which are the predominant cellular fatty acids found in all recognized members of the genus <i>Bacillus</i>. The 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANI), and in silico DNA—DNA hybridization (DDH) value between strain 03113<sup>T</sup> and the most closely related species were 70.5% and 22.6%, respectively, which supported our conclusion that 03113<sup>T</sup> represented a novel species in the genus <i>Bacillus</i>. We demonstrated that type strain 03113<sup>T</sup> (=ACCC 03113<sup>T</sup>=JCM 33017<sup>T</sup>) was a novel species in the genus <i>Bacillus</i>, and the name <i>Bacillus telluris</i> sp. nov. was proposed. Strain 03113<sup>T</sup> secreted auxin IAA and carried the nitrogenase iron protein (<i>nifH</i>) gene, which indicated that strain 03113<sup>T</sup> has the potential to fix nitrogen and promote plant growth. <i>Bacillus telluris</i> sp. nov. 03113<sup>T</sup> is a potential candidate for the biofertilizers of organic agriculture areas.https://www.mdpi.com/2076-2607/8/5/702<i>Bacillus telluris</i> sp. nov.genome analysisplant-growth promoting rhizobacterium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
He-Bao Guo Shan-Wen He Xing Wang Kyu-Kyu Thin Hai-Lei Wei Xiao-Xia Zhang |
spellingShingle |
He-Bao Guo Shan-Wen He Xing Wang Kyu-Kyu Thin Hai-Lei Wei Xiao-Xia Zhang <i>Bacillus telluris</i> sp. nov. Isolated from Greenhouse Soil in Beijing, China Microorganisms <i>Bacillus telluris</i> sp. nov. genome analysis plant-growth promoting rhizobacterium |
author_facet |
He-Bao Guo Shan-Wen He Xing Wang Kyu-Kyu Thin Hai-Lei Wei Xiao-Xia Zhang |
author_sort |
He-Bao Guo |
title |
<i>Bacillus telluris</i> sp. nov. Isolated from Greenhouse Soil in Beijing, China |
title_short |
<i>Bacillus telluris</i> sp. nov. Isolated from Greenhouse Soil in Beijing, China |
title_full |
<i>Bacillus telluris</i> sp. nov. Isolated from Greenhouse Soil in Beijing, China |
title_fullStr |
<i>Bacillus telluris</i> sp. nov. Isolated from Greenhouse Soil in Beijing, China |
title_full_unstemmed |
<i>Bacillus telluris</i> sp. nov. Isolated from Greenhouse Soil in Beijing, China |
title_sort |
<i>bacillus telluris</i> sp. nov. isolated from greenhouse soil in beijing, china |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-05-01 |
description |
A novel Gram-stain-positive, rod-shaped, endospore-forming bacterium, which we designated as strain 03113<sup>T</sup>, was isolated from greenhouse soil in Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed strain 03113<sup>T</sup> is in the genus <i>Bacillus</i> and had the highest similarity to <i>Bacillus solani</i> CCTCC AB 2014277<sup>T</sup> (98.14%). The strain grew at 4 °C–50 °C (optimum 37 °C), with 0–10% (<i>w/v</i>) NaCl (optimum 5%), and in the range of pH 3.0–12.0 (optimum pH 8.0). Menaquinone was identified as MK-7, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The main major cellular fatty acids detected were anteiso-C<sub>15:0</sub> (51.35%) and iso-C<sub>15:0</sub> (11.06%), which are the predominant cellular fatty acids found in all recognized members of the genus <i>Bacillus</i>. The 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANI), and in silico DNA—DNA hybridization (DDH) value between strain 03113<sup>T</sup> and the most closely related species were 70.5% and 22.6%, respectively, which supported our conclusion that 03113<sup>T</sup> represented a novel species in the genus <i>Bacillus</i>. We demonstrated that type strain 03113<sup>T</sup> (=ACCC 03113<sup>T</sup>=JCM 33017<sup>T</sup>) was a novel species in the genus <i>Bacillus</i>, and the name <i>Bacillus telluris</i> sp. nov. was proposed. Strain 03113<sup>T</sup> secreted auxin IAA and carried the nitrogenase iron protein (<i>nifH</i>) gene, which indicated that strain 03113<sup>T</sup> has the potential to fix nitrogen and promote plant growth. <i>Bacillus telluris</i> sp. nov. 03113<sup>T</sup> is a potential candidate for the biofertilizers of organic agriculture areas. |
topic |
<i>Bacillus telluris</i> sp. nov. genome analysis plant-growth promoting rhizobacterium |
url |
https://www.mdpi.com/2076-2607/8/5/702 |
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