Transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in Tibetan wild barley
Abstract Background Development of crop cultivars with high low nitrogen (LN) tolerance or nitrogen use efficiency (NUE) is imperative for sustainable agriculture development. Tibetan wild barley is rich in genetic diversity and may provide elite genes for LN tolerance improvement. Little has been k...
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doaj-c1e7019f3e804639adf773cf945706792020-11-25T02:05:50ZengBMCBMC Plant Biology1471-22292019-02-0119111210.1186/s12870-019-1668-3Transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in Tibetan wild barleyXiaoyan Quan0Jianbin Zeng1Guang Chen2Guoping Zhang3Agronomy Department, Institute of Crop Science, Zhejiang UniversityAgronomy Department, Institute of Crop Science, Zhejiang UniversityAgronomy Department, Institute of Crop Science, Zhejiang UniversityAgronomy Department, Institute of Crop Science, Zhejiang UniversityAbstract Background Development of crop cultivars with high low nitrogen (LN) tolerance or nitrogen use efficiency (NUE) is imperative for sustainable agriculture development. Tibetan wild barley is rich in genetic diversity and may provide elite genes for LN tolerance improvement. Little has been known about transcriptional responses of the wild barley to chronic LN stress. Results In this study, transcriptomic profiling of two Tibetan wild barley genotypes, LN- tolerant XZ149 and LN-sensitive XZ56 has been conducted using RNA-Seq to reveal the genotypic difference in response to chronic LN stress. A total of 520 differentially expressed genes (DEGs) were identified in the two genotypes at 12 d after LN stress, and these DEGs could be mainly mapped to 49 metabolism pathways. Chronic LN stress lead to genotype-dependent responses, and the responsive pattern in favor of root growth and stress tolerance may be the possible mechanisms of the higher chronic LN tolerance in XZ149. Conclusion There was a distinct difference in transcriptional profiling between the two wild barley genotypes in response to chronic LN stress. The identified new candidate genes related to LN tolerance may cast a light on the development of cultivars with LN tolerance.http://link.springer.com/article/10.1186/s12870-019-1668-3BarleyChronic low N stressLow N toleranceRNA-SeqGenotypesDifferentially expressed genes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoyan Quan Jianbin Zeng Guang Chen Guoping Zhang |
spellingShingle |
Xiaoyan Quan Jianbin Zeng Guang Chen Guoping Zhang Transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in Tibetan wild barley BMC Plant Biology Barley Chronic low N stress Low N tolerance RNA-Seq Genotypes Differentially expressed genes |
author_facet |
Xiaoyan Quan Jianbin Zeng Guang Chen Guoping Zhang |
author_sort |
Xiaoyan Quan |
title |
Transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in Tibetan wild barley |
title_short |
Transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in Tibetan wild barley |
title_full |
Transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in Tibetan wild barley |
title_fullStr |
Transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in Tibetan wild barley |
title_full_unstemmed |
Transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in Tibetan wild barley |
title_sort |
transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in tibetan wild barley |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2019-02-01 |
description |
Abstract Background Development of crop cultivars with high low nitrogen (LN) tolerance or nitrogen use efficiency (NUE) is imperative for sustainable agriculture development. Tibetan wild barley is rich in genetic diversity and may provide elite genes for LN tolerance improvement. Little has been known about transcriptional responses of the wild barley to chronic LN stress. Results In this study, transcriptomic profiling of two Tibetan wild barley genotypes, LN- tolerant XZ149 and LN-sensitive XZ56 has been conducted using RNA-Seq to reveal the genotypic difference in response to chronic LN stress. A total of 520 differentially expressed genes (DEGs) were identified in the two genotypes at 12 d after LN stress, and these DEGs could be mainly mapped to 49 metabolism pathways. Chronic LN stress lead to genotype-dependent responses, and the responsive pattern in favor of root growth and stress tolerance may be the possible mechanisms of the higher chronic LN tolerance in XZ149. Conclusion There was a distinct difference in transcriptional profiling between the two wild barley genotypes in response to chronic LN stress. The identified new candidate genes related to LN tolerance may cast a light on the development of cultivars with LN tolerance. |
topic |
Barley Chronic low N stress Low N tolerance RNA-Seq Genotypes Differentially expressed genes |
url |
http://link.springer.com/article/10.1186/s12870-019-1668-3 |
work_keys_str_mv |
AT xiaoyanquan transcriptomicanalysisrevealsadaptivestrategiestochroniclownitrogenintibetanwildbarley AT jianbinzeng transcriptomicanalysisrevealsadaptivestrategiestochroniclownitrogenintibetanwildbarley AT guangchen transcriptomicanalysisrevealsadaptivestrategiestochroniclownitrogenintibetanwildbarley AT guopingzhang transcriptomicanalysisrevealsadaptivestrategiestochroniclownitrogenintibetanwildbarley |
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1724936700664217600 |