The heterogeneic distribution of Helicobacter pylori cag pathogenicity island reflects different pathologies in multiracial Malaysian population
Background: Helicobacter pylori harbouring cag-pathogenicity island (cagPAI) which encodes type IV secretion system (T4SS) and cagA virulence gene are involved in inflammation of the gastric mucosa. We examined all the 27 cagPAI genes in 88 H. pylori isolates from patients of different ethnicities a...
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doaj-c1f4c416db1848a68d442958417d0c582020-12-11T04:20:36ZengElsevierBrazilian Journal of Infectious Diseases1413-86702020-11-01246545551The heterogeneic distribution of Helicobacter pylori cag pathogenicity island reflects different pathologies in multiracial Malaysian populationAlfizah Hanafiah0Shaza Azlin Razak1Hui-min Neoh2Noraziah Mohamad Zin3Bruno S. Lopes4Universiti Kebangsaan Malaysia, Faculty of Medicine, Dept. of Medical Microbiology and Immunology, Kuala Lumpur, Malaysia; Corresponding author.Universiti Kebangsaan Malaysia, Faculty of Medicine, Dept. of Medical Microbiology and Immunology, Kuala Lumpur, MalaysiaUKM Medical Molecular Biology Institute, Kuala Lumpur, MalaysiaUniversiti Kebangsaan Malaysia, Faculty of Health Sciences, School of Diagnostic Science and Applied Health, Kuala Lumpur, MalaysiaUniversity of Aberdeen, School of Medicine, Medical Sciences and Nutrition Aberdeen, Dept. of Medical Microbiology, United KingdomBackground: Helicobacter pylori harbouring cag-pathogenicity island (cagPAI) which encodes type IV secretion system (T4SS) and cagA virulence gene are involved in inflammation of the gastric mucosa. We examined all the 27 cagPAI genes in 88 H. pylori isolates from patients of different ethnicities and examined the association of the intactness of cagPAI region with histopathological scores of the gastric mucosa. Results: 96.6% (n = 85) of H. pylori isolates were cagPAI-positive with 22.4% (19/85) having an intact cagPAI, whereas 77.6% (66/85) had a partial/rearranged cagPAI. The frequency of cag2 and cag14 were found to be significantly higher in H. pylori isolated from Malays, whereas cag4 was predominantly found in Chinese isolates. The cag24 was significantly found in higher proportions in Malay and Indian isolates than in Chinese isolates. The intactness of cagPAI region showed an association with histopathological scores of the gastric mucosa. Significant association was observed between H. pylori harbouring partial cagPAI with higher density of bacteria and neutrophil activity, whereas strains lacking cagPAI were associated with higher inflammatory score. Conclusions: The genotypes of H. pylori strains with various cagPAI rearrangement associated with patients’ ethnicities and histopathological scores might contribute to the pathogenesis of H. pylori infection in a multi-ethnic population.http://www.sciencedirect.com/science/article/pii/S1413867020301574Helicobacter pyloricagPAIHistopathological scoresGastric mucosaEthinicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alfizah Hanafiah Shaza Azlin Razak Hui-min Neoh Noraziah Mohamad Zin Bruno S. Lopes |
spellingShingle |
Alfizah Hanafiah Shaza Azlin Razak Hui-min Neoh Noraziah Mohamad Zin Bruno S. Lopes The heterogeneic distribution of Helicobacter pylori cag pathogenicity island reflects different pathologies in multiracial Malaysian population Brazilian Journal of Infectious Diseases Helicobacter pylori cagPAI Histopathological scores Gastric mucosa Ethinicity |
author_facet |
Alfizah Hanafiah Shaza Azlin Razak Hui-min Neoh Noraziah Mohamad Zin Bruno S. Lopes |
author_sort |
Alfizah Hanafiah |
title |
The heterogeneic distribution of Helicobacter pylori cag pathogenicity island reflects different pathologies in multiracial Malaysian population |
title_short |
The heterogeneic distribution of Helicobacter pylori cag pathogenicity island reflects different pathologies in multiracial Malaysian population |
title_full |
The heterogeneic distribution of Helicobacter pylori cag pathogenicity island reflects different pathologies in multiracial Malaysian population |
title_fullStr |
The heterogeneic distribution of Helicobacter pylori cag pathogenicity island reflects different pathologies in multiracial Malaysian population |
title_full_unstemmed |
The heterogeneic distribution of Helicobacter pylori cag pathogenicity island reflects different pathologies in multiracial Malaysian population |
title_sort |
heterogeneic distribution of helicobacter pylori cag pathogenicity island reflects different pathologies in multiracial malaysian population |
publisher |
Elsevier |
series |
Brazilian Journal of Infectious Diseases |
issn |
1413-8670 |
publishDate |
2020-11-01 |
description |
Background: Helicobacter pylori harbouring cag-pathogenicity island (cagPAI) which encodes type IV secretion system (T4SS) and cagA virulence gene are involved in inflammation of the gastric mucosa. We examined all the 27 cagPAI genes in 88 H. pylori isolates from patients of different ethnicities and examined the association of the intactness of cagPAI region with histopathological scores of the gastric mucosa. Results: 96.6% (n = 85) of H. pylori isolates were cagPAI-positive with 22.4% (19/85) having an intact cagPAI, whereas 77.6% (66/85) had a partial/rearranged cagPAI. The frequency of cag2 and cag14 were found to be significantly higher in H. pylori isolated from Malays, whereas cag4 was predominantly found in Chinese isolates. The cag24 was significantly found in higher proportions in Malay and Indian isolates than in Chinese isolates. The intactness of cagPAI region showed an association with histopathological scores of the gastric mucosa. Significant association was observed between H. pylori harbouring partial cagPAI with higher density of bacteria and neutrophil activity, whereas strains lacking cagPAI were associated with higher inflammatory score. Conclusions: The genotypes of H. pylori strains with various cagPAI rearrangement associated with patients’ ethnicities and histopathological scores might contribute to the pathogenesis of H. pylori infection in a multi-ethnic population. |
topic |
Helicobacter pylori cagPAI Histopathological scores Gastric mucosa Ethinicity |
url |
http://www.sciencedirect.com/science/article/pii/S1413867020301574 |
work_keys_str_mv |
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