Using cAMP Sensors to Study Cardiac Nanodomains
3′,5′-cyclic adenosine monophosphate (cAMP) signalling plays a major role in the cardiac myocyte response to extracellular stimulation by hormones and neurotransmitters. In recent years, evidence has accumulated demonstrating that the cAMP response to different extracellular agonists is not uniform:...
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MDPI AG
2018-03-01
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| Series: | Journal of Cardiovascular Development and Disease |
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| Online Access: | http://www.mdpi.com/2308-3425/5/1/17 |
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doaj-c2013966d2a642328ed7f69c9b9e62e12020-11-24T22:04:07ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252018-03-01511710.3390/jcdd5010017jcdd5010017Using cAMP Sensors to Study Cardiac NanodomainsKatharina Schleicher0Manuela Zaccolo1Department of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, South Parks Road, Oxford OX1 3PT, UKDepartment of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, South Parks Road, Oxford OX1 3PT, UK3′,5′-cyclic adenosine monophosphate (cAMP) signalling plays a major role in the cardiac myocyte response to extracellular stimulation by hormones and neurotransmitters. In recent years, evidence has accumulated demonstrating that the cAMP response to different extracellular agonists is not uniform: depending on the stimulus, cAMP signals of different amplitudes and kinetics are generated in different subcellular compartments, eliciting defined physiological effects. In this review, we focus on how real-time imaging using fluorescence resonance energy transfer (FRET)-based reporters has provided mechanistic insight into the compartmentalisation of the cAMP signalling pathway and allowed for the precise definition of the regulation and function of subcellular cAMP nanodomains.http://www.mdpi.com/2308-3425/5/1/173′,5′-cyclic adenosine monophosphateprotein kinase Afluorescence resonance energy transferreal-time imagingcompartmentalisationsignallingcardiac biologyphosphodiesterasesA kinase anchoring proteins |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Katharina Schleicher Manuela Zaccolo |
| spellingShingle |
Katharina Schleicher Manuela Zaccolo Using cAMP Sensors to Study Cardiac Nanodomains Journal of Cardiovascular Development and Disease 3′,5′-cyclic adenosine monophosphate protein kinase A fluorescence resonance energy transfer real-time imaging compartmentalisation signalling cardiac biology phosphodiesterases A kinase anchoring proteins |
| author_facet |
Katharina Schleicher Manuela Zaccolo |
| author_sort |
Katharina Schleicher |
| title |
Using cAMP Sensors to Study Cardiac Nanodomains |
| title_short |
Using cAMP Sensors to Study Cardiac Nanodomains |
| title_full |
Using cAMP Sensors to Study Cardiac Nanodomains |
| title_fullStr |
Using cAMP Sensors to Study Cardiac Nanodomains |
| title_full_unstemmed |
Using cAMP Sensors to Study Cardiac Nanodomains |
| title_sort |
using camp sensors to study cardiac nanodomains |
| publisher |
MDPI AG |
| series |
Journal of Cardiovascular Development and Disease |
| issn |
2308-3425 |
| publishDate |
2018-03-01 |
| description |
3′,5′-cyclic adenosine monophosphate (cAMP) signalling plays a major role in the cardiac myocyte response to extracellular stimulation by hormones and neurotransmitters. In recent years, evidence has accumulated demonstrating that the cAMP response to different extracellular agonists is not uniform: depending on the stimulus, cAMP signals of different amplitudes and kinetics are generated in different subcellular compartments, eliciting defined physiological effects. In this review, we focus on how real-time imaging using fluorescence resonance energy transfer (FRET)-based reporters has provided mechanistic insight into the compartmentalisation of the cAMP signalling pathway and allowed for the precise definition of the regulation and function of subcellular cAMP nanodomains. |
| topic |
3′,5′-cyclic adenosine monophosphate protein kinase A fluorescence resonance energy transfer real-time imaging compartmentalisation signalling cardiac biology phosphodiesterases A kinase anchoring proteins |
| url |
http://www.mdpi.com/2308-3425/5/1/17 |
| work_keys_str_mv |
AT katharinaschleicher usingcampsensorstostudycardiacnanodomains AT manuelazaccolo usingcampsensorstostudycardiacnanodomains |
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