Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola)
Downy mildew (Plasmopara viticola) is the main grapevine disease in humid regions. In the present investigation, marker-assisted selection (MAS) was used to develop grapevine lines homozygous in loci Rpv1 and Rpv3 for resistance against P. viticola. The experimental populations UFSC-2013-1 (n = 420...
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Brazilian Society of Plant Breeding
2017-04-01
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doaj-c2103d266a7641a3aff03aebd1b3be142020-11-24T21:21:17ZengBrazilian Society of Plant BreedingCrop Breeding and Applied Biotechnology1518-78531984-70332017-04-0117214114910.1590/1984-70332017v17n2a21Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola)Fernando D. Sánchez-Mora0Luciano Saifert1Jean Zanghelini2Wilson T. Assumpção3Cláudia A. Guginski-Piva4Renan Giacomet5Eduardo I. Novak6Gustavo H. Klabunde7Rudolf Eibach8Lirio Dal Vesco9Rubens O. Nodari10Leocir J. Welter11 Universidad Técnica de Manabí, Facultad de Ingeniería Agronómica, Campus Experimental La Teodomira, km 13.5, vía Santa Ana, Manabí, EcuadorUniversidade Federal de Santa Catarina (UFSC), Centro de Ciências Agrárias (CCA), Rod. Admar Gonzaga, 1346, Bairro Itacorubi, 88.034-001, Florianópolis, SC, Brazil UFSC, Campus de Curitibanos, Centro de Ciências Rurais, Rod. Ulysses Gaboardi, km 3, 89.520-000, Curitibanos, SC, Brazil UFSC, Campus de Curitibanos, Centro de Ciências Rurais, Rod. Ulysses Gaboardi, km 3, 89.520-000, Curitibanos, SC, Brazil UFSC, Campus de Curitibanos, Centro de Ciências Rurais, Rod. Ulysses Gaboardi, km 3, 89.520-000, Curitibanos, SC, Brazil UFSC, Campus de Curitibanos, Centro de Ciências Rurais, Rod. Ulysses Gaboardi, km 3, 89.520-000, Curitibanos, SC, Brazil UFSC, Campus de Curitibanos, Centro de Ciências Rurais, Rod. Ulysses Gaboardi, km 3, 89.520-000, Curitibanos, SC, BrazilUniversidade Federal de Santa Catarina (UFSC), Centro de Ciências Agrárias (CCA), Rod. Admar Gonzaga, 1346, Bairro Itacorubi, 88.034-001, Florianópolis, SC, Brazil JKI Institute for Grapevine Breeding Geilweilerhof, 76833, Siebeldingen, Germany JKI Institute for Grapevine Breeding Geilweilerhof, 76833, Siebeldingen, GermanyUniversidade Federal de Santa Catarina (UFSC), Centro de Ciências Agrárias (CCA), Rod. Admar Gonzaga, 1346, Bairro Itacorubi, 88.034-001, Florianópolis, SC, Brazil UFSC, Campus de Curitibanos, Centro de Ciências Rurais, Rod. Ulysses Gaboardi, km 3, 89.520-000, Curitibanos, SC, BrazilDowny mildew (Plasmopara viticola) is the main grapevine disease in humid regions. In the present investigation, marker-assisted selection (MAS) was used to develop grapevine lines homozygous in loci Rpv1 and Rpv3 for resistance against P. viticola. The experimental populations UFSC-2013-1 (n = 420) and UFSC-2013-2 (n = 237) were obtained by self-pollination of two F1 full-sib plants, originated from a cross between two distinct breeding lines containing the downy mildew resistance loci Rpv1 and Rpv3 in heterozygosity. The two experimental populations were genotyped with four microsatellite markers flanking the two downy mildew resistance loci. Among 637 genotyped plants, 300 (48.2%) were homozygous for at least one resistance locus and 10 (1.57%) were homozygous for both Rpv1 and Rpv3 loci. These 10 plants challenged with P. viticola inoculum showed a clearly enhanced level of resistance. These plants have a great potential as resistance donors in grapevine breeding.http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-a778-11eb.pdfVitisdisease resistancegene pyramidingdistorted segregation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fernando D. Sánchez-Mora Luciano Saifert Jean Zanghelini Wilson T. Assumpção Cláudia A. Guginski-Piva Renan Giacomet Eduardo I. Novak Gustavo H. Klabunde Rudolf Eibach Lirio Dal Vesco Rubens O. Nodari Leocir J. Welter |
spellingShingle |
Fernando D. Sánchez-Mora Luciano Saifert Jean Zanghelini Wilson T. Assumpção Cláudia A. Guginski-Piva Renan Giacomet Eduardo I. Novak Gustavo H. Klabunde Rudolf Eibach Lirio Dal Vesco Rubens O. Nodari Leocir J. Welter Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola) Crop Breeding and Applied Biotechnology Vitis disease resistance gene pyramiding distorted segregation |
author_facet |
Fernando D. Sánchez-Mora Luciano Saifert Jean Zanghelini Wilson T. Assumpção Cláudia A. Guginski-Piva Renan Giacomet Eduardo I. Novak Gustavo H. Klabunde Rudolf Eibach Lirio Dal Vesco Rubens O. Nodari Leocir J. Welter |
author_sort |
Fernando D. Sánchez-Mora |
title |
Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola) |
title_short |
Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola) |
title_full |
Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola) |
title_fullStr |
Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola) |
title_full_unstemmed |
Behavior of grape breeding lines with distinct resistance alleles to downy mildew (Plasmopara viticola) |
title_sort |
behavior of grape breeding lines with distinct resistance alleles to downy mildew (plasmopara viticola) |
publisher |
Brazilian Society of Plant Breeding |
series |
Crop Breeding and Applied Biotechnology |
issn |
1518-7853 1984-7033 |
publishDate |
2017-04-01 |
description |
Downy mildew (Plasmopara viticola) is the main grapevine disease in humid regions. In the present investigation, marker-assisted selection (MAS) was used to develop grapevine lines homozygous in loci Rpv1 and Rpv3 for resistance
against P. viticola. The experimental populations UFSC-2013-1 (n = 420) and UFSC-2013-2 (n = 237) were obtained by self-pollination of two F1 full-sib plants, originated from a cross between two distinct breeding lines containing the downy mildew resistance loci Rpv1 and Rpv3 in heterozygosity. The two experimental populations were genotyped with four microsatellite markers flanking the two downy mildew resistance loci. Among 637 genotyped plants, 300 (48.2%) were homozygous for at least one resistance locus and 10 (1.57%) were homozygous for both Rpv1 and Rpv3 loci. These 10 plants challenged with P. viticola inoculum showed a clearly enhanced level of resistance. These plants have a great potential as resistance donors in grapevine breeding. |
topic |
Vitis disease resistance gene pyramiding distorted segregation |
url |
http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-a778-11eb.pdf |
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