Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms
The development of breast cancer is influenced by the adipose tissue through the proteins leptin and adiponectin. However, there is little research concerning AdipoR1 and AdipoR2 receptors and the influence of leptin over them. The objective of this work was to analyze the expression of AdipoR1 and...
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doaj-c215743b63934c4f81dc45c0f92417e62020-11-24T23:27:56ZengHindawi LimitedDisease Markers0278-02401875-86302017-01-01201710.1155/2017/48620164862016Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation MechanismsMartha Daniela Mociño-Rodríguez0Jonnathan Guadalupe Santillán-Benítez1David Salomón Dozal-Domínguez2María Dolores Hernández-Navarro3Miriam Veronica Flores-Merino4Antonio Sandoval-Cabrera5Francisco Javier García Vázquez6Faculty of Chemistry, Autonomous University of the State of Mexico (UAEMex), Toluca, MEX, MexicoFaculty of Chemistry, Autonomous University of the State of Mexico (UAEMex), Toluca, MEX, MexicoSpecialized Hemato-Oncological Laboratory of the Children’s Hospital, IMIEM, Toluca, MEX, MexicoFaculty of Chemistry, Autonomous University of the State of Mexico (UAEMex), Toluca, MEX, MexicoMolecular Biology Laboratory, Medical Research Center (CICMED), Toluca, MEX, MexicoSpecialized Hemato-Oncological Laboratory of the Children’s Hospital, IMIEM, Toluca, MEX, MexicoLaboratory of Immunohistochemistry, National Pediatrics Institute, Coyoacán, MEX, MexicoThe development of breast cancer is influenced by the adipose tissue through the proteins leptin and adiponectin. However, there is little research concerning AdipoR1 and AdipoR2 receptors and the influence of leptin over them. The objective of this work was to analyze the expression of AdipoR1 and AdipoR2, modulated by differential concentrations of leptin in an obesity model (10 ng/mL, 100 ng/mL, and 1000 ng/mL) associated with breast cancer in MCF-7 and HCC1937 cell lines. Each cell line was characterized through immunohistochemistry, and the expression of AdipoR1 and AdipoR2 was analyzed by PCR in real time using TaqMan® probes. Leptin induced an increase in cell population of MCF-7 (23.8%, 10 ng/mL, 48 h) and HCC1937 (17.24%, 1000 ng/mL, 72 h). In MCF-7, the expression of AdipoR1 decreased (3.81%, 1000 ng/mL) and the expression of AdipoR2 increased by 13.74 times (10 ng/mL) with regard to the control. In HCC1937, the expression of AdipoR1 decreased by 86.28% (10 ng/mL), as well as the expression of AdipoR2 (50.3%, 100 ng/mL). In regard to the results obtained, it could be concluded that leptin has an effect over the expression of AdipoR1 and AdipoR2 mRNA.http://dx.doi.org/10.1155/2017/4862016 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Martha Daniela Mociño-Rodríguez Jonnathan Guadalupe Santillán-Benítez David Salomón Dozal-Domínguez María Dolores Hernández-Navarro Miriam Veronica Flores-Merino Antonio Sandoval-Cabrera Francisco Javier García Vázquez |
spellingShingle |
Martha Daniela Mociño-Rodríguez Jonnathan Guadalupe Santillán-Benítez David Salomón Dozal-Domínguez María Dolores Hernández-Navarro Miriam Veronica Flores-Merino Antonio Sandoval-Cabrera Francisco Javier García Vázquez Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms Disease Markers |
author_facet |
Martha Daniela Mociño-Rodríguez Jonnathan Guadalupe Santillán-Benítez David Salomón Dozal-Domínguez María Dolores Hernández-Navarro Miriam Veronica Flores-Merino Antonio Sandoval-Cabrera Francisco Javier García Vázquez |
author_sort |
Martha Daniela Mociño-Rodríguez |
title |
Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms |
title_short |
Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms |
title_full |
Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms |
title_fullStr |
Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms |
title_full_unstemmed |
Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer Regulation Mechanisms |
title_sort |
expression of adipor1 and adipor2 receptors as leptin-breast cancer regulation mechanisms |
publisher |
Hindawi Limited |
series |
Disease Markers |
issn |
0278-0240 1875-8630 |
publishDate |
2017-01-01 |
description |
The development of breast cancer is influenced by the adipose tissue through the proteins leptin and adiponectin. However, there is little research concerning AdipoR1 and AdipoR2 receptors and the influence of leptin over them. The objective of this work was to analyze the expression of AdipoR1 and AdipoR2, modulated by differential concentrations of leptin in an obesity model (10 ng/mL, 100 ng/mL, and 1000 ng/mL) associated with breast cancer in MCF-7 and HCC1937 cell lines. Each cell line was characterized through immunohistochemistry, and the expression of AdipoR1 and AdipoR2 was analyzed by PCR in real time using TaqMan® probes. Leptin induced an increase in cell population of MCF-7 (23.8%, 10 ng/mL, 48 h) and HCC1937 (17.24%, 1000 ng/mL, 72 h). In MCF-7, the expression of AdipoR1 decreased (3.81%, 1000 ng/mL) and the expression of AdipoR2 increased by 13.74 times (10 ng/mL) with regard to the control. In HCC1937, the expression of AdipoR1 decreased by 86.28% (10 ng/mL), as well as the expression of AdipoR2 (50.3%, 100 ng/mL). In regard to the results obtained, it could be concluded that leptin has an effect over the expression of AdipoR1 and AdipoR2 mRNA. |
url |
http://dx.doi.org/10.1155/2017/4862016 |
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