Application of the Rapid Linear Immune-Electrophoresis for Differentiation between Burkholderia

• Main Authors: I. I. Korsakova, N. P. Khrapova, G. M. Napalkova, L. V. Lomova, T. V. Bulatova
• Format: Article
• Language: Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2014-06-01
• Series: Проблемы особо опасных инфекций
• Subjects: сап; мелиоидоз; патогенные и непатогенные буркхольдерии; дифференциация; ускоренный линейный иммуноэлектрофорез; glanders; melioidosis; pathogenic and non-pathogenic burkholderia; differentiation; rapid linear immune-electrophoresis
• Online Access: https://journal.microbe.ru/jour/article/view/147

Objective of the study was to develop a method that would allow for rapid linear immune-electrophoresis to differentiate between pathogenic agents of glanders and melioidosis and non-pathogenic closely-related Burkholderia . The put-forward modification of the technique made it possible to detect the antigens of pathogenic B. pseudomallei and B. mallei due to the presence of precipitation lines in between the sample gel and the one with immune sera. B. thailandensis, B. cepacia, and B. gladioli did not form such precipitation lines, which in its turn provided for the possibility to differentiate between the mentioned ones and pathogenic Burkholderia. The rapid enhanced linear immune-electrophoresis is easy to perform and compelling, and takes little time. It is qualified for identification of heterogenic and specific antigens in Burkholderia , selection of immune sera containing antibodies to the existing antigens, and can be used as a supportive alternative analytical means for the detection of antigens of pathogenic Burkholderia .