Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs
<p>This study aimed to compare the quality and the penetration ability of frozen–thawed spermatozoa from three microminipigs and Large White boars and to evaluate the effects of caffeine and heparin as well as the sperm–oocyte co-incubation length on the fertilization and embryonic development...
Main Authors: | , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Copernicus Publications
2021-06-01
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Series: | Archives Animal Breeding |
Online Access: | https://aab.copernicus.org/articles/64/265/2021/aab-64-265-2021.pdf |
Summary: | <p>This study aimed to compare the quality and the penetration
ability of frozen–thawed spermatozoa from three microminipigs and Large White
boars and to evaluate the effects of caffeine and heparin as well as the
sperm–oocyte co-incubation length on the fertilization and embryonic
development in vitro. Results showed that the fertilization rates of
spermatozoa from three microminipig boars were significantly lower than
those of a Large White boar. In the post-thaw spermatozoa from one of three
microminipig boars, the sperm quality, penetration ability, and the oocyte
development after in vitro fertilization were significantly lower than those
of the spermatozoa from other boars. The caffeine supplementation in the
fertilization media increased the rates of fertilization and blastocyst
formation for the microminipig spermatozoa with low sperm quality. In
addition to caffeine supplementation, the rates of fertilization and
blastocyst formation after using microminipig spermatozoa were significantly
higher with a 10 h sperm–oocyte co-incubation than with 3 h of
co-incubation length. Our results indicate that the differences between the
males and the breed influence the quality and fertility of frozen–thawed
boar spermatozoa. In conclusion, the presence of caffeine in the in vitro fertilization (IVF) medium
and adequate length of sperm–oocyte co-incubation may have beneficial
effects for improving IVF results when using microminipig spermatozoa with
low quality.</p> |
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ISSN: | 0003-9438 2363-9822 |