Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma
<p>Abstract</p> <p>Background</p> <p>The development of esophageal squamous cell carcinoma (ESCC) progresses a multistage process, collectively known as precursor lesions, also called dysplasia (DYS) and carcinoma <it>in situ </it>(CIS), subsequent invasive...
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doaj-c2348d33673b4c36a042081d16dac41b2020-11-24T21:13:49ZengBMCBMC Cancer1471-24072006-12-016129610.1186/1471-2407-6-296Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinomaHe Zu-genTong TongLin Dong-meiRen Li-qunQian Lu-xiaShou Jian-zhongZou Shuang-meiSong Yong-meiHu NanXue Li-yanZhan Qi-minTaylor Philip RLu Ning<p>Abstract</p> <p>Background</p> <p>The development of esophageal squamous cell carcinoma (ESCC) progresses a multistage process, collectively known as precursor lesions, also called dysplasia (DYS) and carcinoma <it>in situ </it>(CIS), subsequent invasive lesions and final metastasis. In this study, we are interested in investigating the expression of a variety of functional classes of proteins in ESCC and its precursor lesions and characterizing the correlation of these proteins with ESCC malignant progression.</p> <p>Methods</p> <p>Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC were analyzed using immunohistochemistry on tissue microarray containing 205 ESCC and 173 adjacent precursor lesions as well as corresponding normal mucosa. To confirm the immunohistochemical results, three proteins, fascin, CK14 and laminin-5γ2, which were overexpressed in ESCC on tissue microarray, were detected in 12 ESCC cell lines by Western blot assay.</p> <p>Results</p> <p>In ESCC and its precursor lesions, FADD, CDC25B, fascin, CK14, laminin-5γ2 and SPARC were overexpressed, while Fas, caspase 8, CK4 and annexin I were underexpressed. The abnormalities of these proteins could be classified into different groups in relation to the stages of ESCC development. They were "early" corresponding to mild and moderate DYS with overexpression of fascin, FADD and CDC25B and underexpression of Fas, caspase 8, CK4 and annexin I, "intermediate" to severe DYS and CIS with overexpression of FADD and CK14, and "late" to invasive lesions (ESCC) and to advanced pTNM stage ESCC lesions with overexpression of CK14, laminin-5γ2 and SPARC.</p> <p>Conclusion</p> <p>Analyzing the protein expression patterns of Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC would be valuable to develop rational strategies for early detection of lesions at risk in advance as well as for prevention and treatment of ESCC.</p> http://www.biomedcentral.com/1471-2407/6/296 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
He Zu-gen Tong Tong Lin Dong-mei Ren Li-qun Qian Lu-xia Shou Jian-zhong Zou Shuang-mei Song Yong-mei Hu Nan Xue Li-yan Zhan Qi-min Taylor Philip R Lu Ning |
spellingShingle |
He Zu-gen Tong Tong Lin Dong-mei Ren Li-qun Qian Lu-xia Shou Jian-zhong Zou Shuang-mei Song Yong-mei Hu Nan Xue Li-yan Zhan Qi-min Taylor Philip R Lu Ning Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma BMC Cancer |
author_facet |
He Zu-gen Tong Tong Lin Dong-mei Ren Li-qun Qian Lu-xia Shou Jian-zhong Zou Shuang-mei Song Yong-mei Hu Nan Xue Li-yan Zhan Qi-min Taylor Philip R Lu Ning |
author_sort |
He Zu-gen |
title |
Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma |
title_short |
Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma |
title_full |
Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma |
title_fullStr |
Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma |
title_full_unstemmed |
Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma |
title_sort |
tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2006-12-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The development of esophageal squamous cell carcinoma (ESCC) progresses a multistage process, collectively known as precursor lesions, also called dysplasia (DYS) and carcinoma <it>in situ </it>(CIS), subsequent invasive lesions and final metastasis. In this study, we are interested in investigating the expression of a variety of functional classes of proteins in ESCC and its precursor lesions and characterizing the correlation of these proteins with ESCC malignant progression.</p> <p>Methods</p> <p>Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC were analyzed using immunohistochemistry on tissue microarray containing 205 ESCC and 173 adjacent precursor lesions as well as corresponding normal mucosa. To confirm the immunohistochemical results, three proteins, fascin, CK14 and laminin-5γ2, which were overexpressed in ESCC on tissue microarray, were detected in 12 ESCC cell lines by Western blot assay.</p> <p>Results</p> <p>In ESCC and its precursor lesions, FADD, CDC25B, fascin, CK14, laminin-5γ2 and SPARC were overexpressed, while Fas, caspase 8, CK4 and annexin I were underexpressed. The abnormalities of these proteins could be classified into different groups in relation to the stages of ESCC development. They were "early" corresponding to mild and moderate DYS with overexpression of fascin, FADD and CDC25B and underexpression of Fas, caspase 8, CK4 and annexin I, "intermediate" to severe DYS and CIS with overexpression of FADD and CK14, and "late" to invasive lesions (ESCC) and to advanced pTNM stage ESCC lesions with overexpression of CK14, laminin-5γ2 and SPARC.</p> <p>Conclusion</p> <p>Analyzing the protein expression patterns of Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC would be valuable to develop rational strategies for early detection of lesions at risk in advance as well as for prevention and treatment of ESCC.</p> |
url |
http://www.biomedcentral.com/1471-2407/6/296 |
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