Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits
Plasma lipoproteins of d < 1.063 g/ml from rabbits fed a diet containing 1% cholesterol for 4 days showed changes in concentration and rates of flotation as determined by analytical ultracentrifugation. A marked increase in cholesteryl ester content of lipoprotein with d < 1.019 g/ml was the m...
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doaj-c245146f5df947ac9a005f5fa36731402021-04-24T05:51:19ZengElsevierJournal of Lipid Research0022-22751973-01-011416168Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbitsGermán Camejo0Virgilio Bosch1Carlos Arreaza2Halina C. Mendez3Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, and Cátedras de Bioquímica y Fisiopatología, Instituto de Medicina Experimental, Universidad Central de Venezuela, Caracas, VenezuelaCentro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, and Cátedras de Bioquímica y Fisiopatología, Instituto de Medicina Experimental, Universidad Central de Venezuela, Caracas, VenezuelaCentro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, and Cátedras de Bioquímica y Fisiopatología, Instituto de Medicina Experimental, Universidad Central de Venezuela, Caracas, VenezuelaCentro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, and Cátedras de Bioquímica y Fisiopatología, Instituto de Medicina Experimental, Universidad Central de Venezuela, Caracas, VenezuelaPlasma lipoproteins of d < 1.063 g/ml from rabbits fed a diet containing 1% cholesterol for 4 days showed changes in concentration and rates of flotation as determined by analytical ultracentrifugation. A marked increase in cholesteryl ester content of lipoprotein with d < 1.019 g/ml was the most prominent change in rabbits fed the diet for 21 days. Gel electrophoresis and immunochemical procedures demonstrated that in control and hypercholesterolemic rabbits there were some common apolipoproteins found in all lipoproteins with density < 1.063 g/ml. In control rabbits, there were also apolipoproteins specific to the lipoprotein fraction with d < 1.019 and to the fraction with d 1.019–1.063 g/ml. However, in rabbits fed the hypercholesterolemic diet for 21 days, the apolipoproteins characteristic of fraction 1.019–1.063 were the most abundant in the fraction with d < 1.019 g/ml. Liver slices from rabbits fed the high cholesterol diet for 7 and 21 days incorporated more l-[14C]leucine into very low density and low density lipoproteins than controls.The results suggest that cholesterol feeding leads to an increase in biosynthesis of lipoproteins with d < 1.063 g/ml. The newly synthesized lipoprotein contains apolipoproteins similar to those found in controls but with a higher lipid-to-protein ratio. Fram the apoprotein composition, it is concluded that the very low density fraction present in cholesterol-fed animals is more structurally related to low density lipoproteins than to the very low density lipoproteins isolated from control animals.http://www.sciencedirect.com/science/article/pii/S0022227520393305ultracentrifugationflotationlipid compositionlow density–very low density lipoprotein relationshipapolipoproteinsimmunochemical and gel electrophoresis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Germán Camejo Virgilio Bosch Carlos Arreaza Halina C. Mendez |
spellingShingle |
Germán Camejo Virgilio Bosch Carlos Arreaza Halina C. Mendez Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits Journal of Lipid Research ultracentrifugation flotation lipid composition low density–very low density lipoprotein relationship apolipoproteins immunochemical and gel electrophoresis |
author_facet |
Germán Camejo Virgilio Bosch Carlos Arreaza Halina C. Mendez |
author_sort |
Germán Camejo |
title |
Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits |
title_short |
Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits |
title_full |
Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits |
title_fullStr |
Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits |
title_full_unstemmed |
Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits |
title_sort |
early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1973-01-01 |
description |
Plasma lipoproteins of d < 1.063 g/ml from rabbits fed a diet containing 1% cholesterol for 4 days showed changes in concentration and rates of flotation as determined by analytical ultracentrifugation. A marked increase in cholesteryl ester content of lipoprotein with d < 1.019 g/ml was the most prominent change in rabbits fed the diet for 21 days. Gel electrophoresis and immunochemical procedures demonstrated that in control and hypercholesterolemic rabbits there were some common apolipoproteins found in all lipoproteins with density < 1.063 g/ml. In control rabbits, there were also apolipoproteins specific to the lipoprotein fraction with d < 1.019 and to the fraction with d 1.019–1.063 g/ml. However, in rabbits fed the hypercholesterolemic diet for 21 days, the apolipoproteins characteristic of fraction 1.019–1.063 were the most abundant in the fraction with d < 1.019 g/ml. Liver slices from rabbits fed the high cholesterol diet for 7 and 21 days incorporated more l-[14C]leucine into very low density and low density lipoproteins than controls.The results suggest that cholesterol feeding leads to an increase in biosynthesis of lipoproteins with d < 1.063 g/ml. The newly synthesized lipoprotein contains apolipoproteins similar to those found in controls but with a higher lipid-to-protein ratio. Fram the apoprotein composition, it is concluded that the very low density fraction present in cholesterol-fed animals is more structurally related to low density lipoproteins than to the very low density lipoproteins isolated from control animals. |
topic |
ultracentrifugation flotation lipid composition low density–very low density lipoprotein relationship apolipoproteins immunochemical and gel electrophoresis |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520393305 |
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