Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers
Indoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15....
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Serbian Genetics Society
2013-01-01
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doaj-c26e9eb58d904ea7b4ef268dbcd668e52020-11-24T23:36:33ZengSerbian Genetics SocietyGenetika0534-00121820-60692013-01-0145123724910.2298/GENSR1301237PSelection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubersPoštić DobrivojStarović MiraPopović TatjanaBosnić PredragStanojković-Sebić AleksandraPivić RadmilaJošić DraganaIndoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15.9 µ gmL- 1 after 24h of cultivation and 4.08 to 26.4 µ gmL-1 after 48h of cultivation. The molecular comparison by RAPD analysis also was done. RAPD patterns of selected Pseudomonas spp. isolates obtained by BC318, AF14, SPH 1 and AP 10 primers demonstrated the suitability of RAPD method in distinguishing a high variability among the four isolates (44 to 68%). The effect on the biological viability of potato (industrially important variety Pirol) was observed during the seven weeks of sprouting at the temperature of 18-20°C. Potato tubers treated by the selected isolates formed slightly lower number of sprouts, but statistically higher mean length - up to 129.9% higher than the control. The mean sprouting capacity was 64.5% higher than a control. Obtained results suggested positive effects of selected IAA producing Pseudomonas isolates on the length of potato tubers and sprouting capacity as the parameters which define biological viability. [Projekat Ministarstva nauke Republike Srbije, br. III46007]http://www.doiserbia.nb.rs/img/doi/0534-0012/2013/0534-00121301237P.pdfPseudomonas spp.IAARAPDbiological viabilitypotatotubersproutsprouting capacity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Poštić Dobrivoj Starović Mira Popović Tatjana Bosnić Predrag Stanojković-Sebić Aleksandra Pivić Radmila Jošić Dragana |
spellingShingle |
Poštić Dobrivoj Starović Mira Popović Tatjana Bosnić Predrag Stanojković-Sebić Aleksandra Pivić Radmila Jošić Dragana Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers Genetika Pseudomonas spp. IAA RAPD biological viability potato tuber sprout sprouting capacity |
author_facet |
Poštić Dobrivoj Starović Mira Popović Tatjana Bosnić Predrag Stanojković-Sebić Aleksandra Pivić Radmila Jošić Dragana |
author_sort |
Poštić Dobrivoj |
title |
Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers |
title_short |
Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers |
title_full |
Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers |
title_fullStr |
Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers |
title_full_unstemmed |
Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers |
title_sort |
selection and rapd analysis of pseudomonas ssp. isolates able to improve biological viability of potato seed tubers |
publisher |
Serbian Genetics Society |
series |
Genetika |
issn |
0534-0012 1820-6069 |
publishDate |
2013-01-01 |
description |
Indoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15.9 µ gmL- 1 after 24h of cultivation and 4.08 to 26.4 µ gmL-1 after 48h of cultivation. The molecular comparison by RAPD analysis also was done. RAPD patterns of selected Pseudomonas spp. isolates obtained by BC318, AF14, SPH 1 and AP 10 primers demonstrated the suitability of RAPD method in distinguishing a high variability among the four isolates (44 to 68%). The effect on the biological viability of potato (industrially important variety Pirol) was observed during the seven weeks of sprouting at the temperature of 18-20°C. Potato tubers treated by the selected isolates formed slightly lower number of sprouts, but statistically higher mean length - up to 129.9% higher than the control. The mean sprouting capacity was 64.5% higher than a control. Obtained results suggested positive effects of selected IAA producing Pseudomonas isolates on the length of potato tubers and sprouting capacity as the parameters which define biological viability. [Projekat Ministarstva nauke Republike Srbije, br. III46007] |
topic |
Pseudomonas spp. IAA RAPD biological viability potato tuber sprout sprouting capacity |
url |
http://www.doiserbia.nb.rs/img/doi/0534-0012/2013/0534-00121301237P.pdf |
work_keys_str_mv |
AT posticdobrivoj selectionandrapdanalysisofpseudomonassspisolatesabletoimprovebiologicalviabilityofpotatoseedtubers AT starovicmira selectionandrapdanalysisofpseudomonassspisolatesabletoimprovebiologicalviabilityofpotatoseedtubers AT popovictatjana selectionandrapdanalysisofpseudomonassspisolatesabletoimprovebiologicalviabilityofpotatoseedtubers AT bosnicpredrag selectionandrapdanalysisofpseudomonassspisolatesabletoimprovebiologicalviabilityofpotatoseedtubers AT stanojkovicsebicaleksandra selectionandrapdanalysisofpseudomonassspisolatesabletoimprovebiologicalviabilityofpotatoseedtubers AT pivicradmila selectionandrapdanalysisofpseudomonassspisolatesabletoimprovebiologicalviabilityofpotatoseedtubers AT josicdragana selectionandrapdanalysisofpseudomonassspisolatesabletoimprovebiologicalviabilityofpotatoseedtubers |
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