Determinação simultânea de carbamazepina, fenitoína e fenobarbital em sangue seco em papel por cromatografia líquida de alta eficiência

• Main Authors: Gabriela Martins Silva de Lima, Roberta Zilles Hahn, Cristina Rama, Liliane Rhoden, Paulina Hidalgo, Cleber Álvares da Silva, Marina Venzon Antunes, Rafael Linden
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2014-07-01
• Series: Química Nova
• Subjects: antiepileptic drugs; dried blood spots; HPLC; therapeutic drug monitoring
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000600023&lng=en&tlng=en
• ISSN: 1678-7064

Carbamazepine, phenobarbital and phenytoin were determined in dried blood spots (DBS) by high performance liquid chromatography, after extraction of 8 mm DBS using a mixture of acetonitrile and methanol. Analytes were separated by reversed-phase chromatography, with a run time of 17 minutes. Intra-assay and inter-assay precisions were in the 5.3 to 8.4% and 3.3 to 5.2% ranges, respectively. Accuracy was in the 98.8 to 104.3% range. The method had sensitivity to detect all analytes at levels below minimum therapeutic concentrations. The analytes were stable at 4 ºC and room temperature for up to 12 days and at 45 ºC for 9 days. The method was applied to 14 paired clinical samples of blood serum and DBS.