THE USE OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AS SCREENING TECHNIQUE FOR PECTIN AND PECTIN SUBSTANCES OF DIETARY FIBERS

• Main Authors: G.N. Ignatyeva, Patricia Melgarejo Juan, Sandra Monera Picazo, Desirée Follana Álvarez
• Format: Article
• Language: English
• Published: Slovak University of Agriculture 2015-02-01
• Series: Journal of Microbiology, Biotechnology and Food Sciences
• Subjects: High-performance liquid chromatography method; water soluble pectin fraction; insoluble pectin fraction; polygalacturonic acid; dietary fiber; food; nutrition’s food; pharmaceutical agents
• Online Access: http://www.jmbfs.org/wp-content/uploads/2015/02/Ignatyeva.pdf

The peak of the unspecified compound of quantitative analysis of organic acids in biological materials by using high-performance liquid chromatography was identified. This peak corresponded to polygalacturonic acid. A High-performance liquid chromatographic method was developed to identify and quantify of water soluble pectin fraction and insoluble pectin fraction, galacturonic acid and polygalacturonic acid. The method based on the extraction of free water soluble fraction with phosphate buffer (pH 4.8) or with 0.01 N phosphoric acid and on the acid hydrolysis of insoluble pectin fraction in presence 0.1 N HNO3. The pectin fractions were separated and determined on a C610H column using elution with 0.01 N phosphoric acids and ultraviolet detection at 210 nm. Galacturonic acid and polygalacturonic acid were used as internal standard. The calibration curves were presented for each of the standards. The determinations were performed in the linear range of 0.04÷0.17% w/v for both pectin fractions. Proposed procedure can considered as a rapid method for quantitative pectin determination in the food, food nutrition’s food, and pharmaceutical agents.