MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development
Abstract Background Morphological features are the most common criteria used to select human embryos for transfer to a receptive uterine cavity. However, such characteristics are not valid for embryos in cellular arrest. Even aneuploid embryos can have normal morphology, and some euploid embryos hav...
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doaj-c2a21df77f2d475e9ef0fc7fa0f8e51d2021-07-18T11:47:20ZengBMCReproductive Biology and Endocrinology1477-78272021-07-011911710.1186/s12958-021-00786-1MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of developmentRicardo Josué Acuña-González0Mercedes Olvera-Valencia1Jorge Skiold López-Canales2Jair Lozano-Cuenca3Mauricio Osorio-Caballero4Héctor Flores-Herrera5Department of Immunobioquímica, Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes” (INPerIER)Department of Immunobioquímica, Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes” (INPerIER)Department of Fisiología y Desarrollo Celular, INPerIERDepartment of Fisiología y Desarrollo Celular, INPerIERDepartment of Salud Sexual y Reproductiva, INPerIERDepartment of Immunobioquímica, Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes” (INPerIER)Abstract Background Morphological features are the most common criteria used to select human embryos for transfer to a receptive uterine cavity. However, such characteristics are not valid for embryos in cellular arrest. Even aneuploid embryos can have normal morphology, and some euploid embryos have aberrant morphology. The aim of this study was to quantify the expression profile of hsa-miR-21-3p, -24-1-5p, -191-5p, and -372-5p in culture media on day 5 of in vitro embryo development, and compare the profiles of two groups of media classified by outcome: successful (n = 25) or unsuccessful (n = 25) implantation pregnancy. Methods Fifty patients were accepted in the Department of Reproductive Biology of a Hospital in México City, based on the Institutional inclusion criteria for in vitro fertilization. Embryos were transferred to the women on day 5 of cultivation, and the culture media were collected. RNA was isolated from each culture medium with TRIzol reagent, and microRNA (miRNA) expression was detected through RT-PCR with specific primers. Expression bands were quantified by reading optical density. Results There was a 5.2-fold greater expression of hsa-miR-191-5p in the pregnancy-related culture media (p ≤ 0.001) and a 1.6-fold greater level of hsa-miR-24-1-5p (p = 0.043) in the media corresponding to non-pregnant women. No significant difference existed between the two groups hsa-miR-21-3p (p = 0.38) or hsa-miR-372-5p (p = 0.41). Conclusions Regarding adequate in vitro embryo development, hsa-miR-191-5p could possibly represent a positive biomarker, while has-miR-24-1-5p may indicate poor prognosis. This former miRNA modulates IGF2BP-1 and IGF2R, associated with the implantation window. On the other hand, hsa-miR-24-1-5p may be related to a poor prognosis of human embryo development.https://doi.org/10.1186/s12958-021-00786-1MiRNA expressionEmbryo developmentImplantationEmbryo culture mediaIn vitro fertilization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ricardo Josué Acuña-González Mercedes Olvera-Valencia Jorge Skiold López-Canales Jair Lozano-Cuenca Mauricio Osorio-Caballero Héctor Flores-Herrera |
spellingShingle |
Ricardo Josué Acuña-González Mercedes Olvera-Valencia Jorge Skiold López-Canales Jair Lozano-Cuenca Mauricio Osorio-Caballero Héctor Flores-Herrera MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development Reproductive Biology and Endocrinology MiRNA expression Embryo development Implantation Embryo culture media In vitro fertilization |
author_facet |
Ricardo Josué Acuña-González Mercedes Olvera-Valencia Jorge Skiold López-Canales Jair Lozano-Cuenca Mauricio Osorio-Caballero Héctor Flores-Herrera |
author_sort |
Ricardo Josué Acuña-González |
title |
MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development |
title_short |
MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development |
title_full |
MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development |
title_fullStr |
MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development |
title_full_unstemmed |
MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development |
title_sort |
mir-191-5p is upregulated in culture media of implanted human embryo on day fifth of development |
publisher |
BMC |
series |
Reproductive Biology and Endocrinology |
issn |
1477-7827 |
publishDate |
2021-07-01 |
description |
Abstract Background Morphological features are the most common criteria used to select human embryos for transfer to a receptive uterine cavity. However, such characteristics are not valid for embryos in cellular arrest. Even aneuploid embryos can have normal morphology, and some euploid embryos have aberrant morphology. The aim of this study was to quantify the expression profile of hsa-miR-21-3p, -24-1-5p, -191-5p, and -372-5p in culture media on day 5 of in vitro embryo development, and compare the profiles of two groups of media classified by outcome: successful (n = 25) or unsuccessful (n = 25) implantation pregnancy. Methods Fifty patients were accepted in the Department of Reproductive Biology of a Hospital in México City, based on the Institutional inclusion criteria for in vitro fertilization. Embryos were transferred to the women on day 5 of cultivation, and the culture media were collected. RNA was isolated from each culture medium with TRIzol reagent, and microRNA (miRNA) expression was detected through RT-PCR with specific primers. Expression bands were quantified by reading optical density. Results There was a 5.2-fold greater expression of hsa-miR-191-5p in the pregnancy-related culture media (p ≤ 0.001) and a 1.6-fold greater level of hsa-miR-24-1-5p (p = 0.043) in the media corresponding to non-pregnant women. No significant difference existed between the two groups hsa-miR-21-3p (p = 0.38) or hsa-miR-372-5p (p = 0.41). Conclusions Regarding adequate in vitro embryo development, hsa-miR-191-5p could possibly represent a positive biomarker, while has-miR-24-1-5p may indicate poor prognosis. This former miRNA modulates IGF2BP-1 and IGF2R, associated with the implantation window. On the other hand, hsa-miR-24-1-5p may be related to a poor prognosis of human embryo development. |
topic |
MiRNA expression Embryo development Implantation Embryo culture media In vitro fertilization |
url |
https://doi.org/10.1186/s12958-021-00786-1 |
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