Increased Sensitivity of Detection of <i>RASSF1A</i> and <i>GSTP1</i> DNA Fragments in Serum of Prostate Cancer Patients: Optimisation of Diagnostics Using OBBPA-ddPCR

Increased Sensitivity of Detection of <i>RASSF1A</i> and <i>GSTP1</i> DNA Fragments in Serum of Prostate Cancer Patients: Optimisation of Diagnostics Using OBBPA-ddPCR

Identification of aberrant DNA methylation is a promising tool in prostate cancer (PCa) diagnosis and treatment. In this study, we evaluated a two-step method named optimised bias-based preamplification followed by digital PCR (OBBPA-dPCR). The method was used to identify promoter hypermethylation o...

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Bibliographic Details
Main Authors: Markus Friedemann, Friederike Horn, Katharina Gutewort, Lars Tautz, Carsten Jandeck, Nicole Bechmann, Olga Sukocheva, Manfred P. Wirth, Susanne Fuessel, Mario Menschikowski
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Cancers
Subjects:
liquid biopsy
cancer diagnostics
prostate cancer
circulating cell-free DNA (cfDNA)
DNA methylation
cell-free tumour DNA
Online Access:https://www.mdpi.com/2072-6694/13/17/4459

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