Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats
Renalase is predominantly expressed in the kidney, where it plays a role in catecholamine metabolism and blood pressure regulation. Moderate‐intensity exercise (MEX) has been shown to increase the concentration of renalase in the blood and to reduce renal function in humans. Moreover, such exercise...
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Online Access: | https://doi.org/10.1002/2211-5463.12812 |
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doaj-c2b775ccdbab44388733056d635a49262020-11-25T03:43:04ZengWileyFEBS Open Bio2211-54632020-06-011061005101210.1002/2211-5463.12812Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of ratsKatsuyuki Tokinoya0Yasuko Yoshida1Takehito Sugasawa2Kazuhiro Takekoshi3Graduate School of Comprehensive Human Sciences University of Tsukuba JapanDepartment of Medical Technology Faculty of Health Sciences Tsukuba International University JapanDivision of Clinical Medicine Faculty of Medicine University of Tsukuba JapanDivision of Clinical Medicine Faculty of Medicine University of Tsukuba JapanRenalase is predominantly expressed in the kidney, where it plays a role in catecholamine metabolism and blood pressure regulation. Moderate‐intensity exercise (MEX) has been shown to increase the concentration of renalase in the blood and to reduce renal function in humans. Moreover, such exercise was also reported to increase catecholamine levels. Here, we examined renalase concentration in the blood and renalase expression levels in different organs after MEX in rats. Twelve male Wistar rats were made to run on a treadmill (MEX group) for 60 min at 20 m·min−1, after resting for 15 min. The control group rats were euthanized after resting on the treadmill. Tissue and blood samples were analyzed using western blotting, real‐time RT‐PCR and ELISA. Overall, the concentrations of renalase in the blood were significantly higher in the MEX group than that in the control group. Renalase expression was decreased in the kidney after 60 min of exercise, whereas the expression of renalase mRNA and protein in the extensor digitorum longus and plantaris muscles, respectively, increased after exercise. However, the expression of renalase in the other tissues examined did not change after acute exercise. In conclusion, we report that MEX for 60 min increases both renalase concentration in the blood and its expression in skeletal muscle.https://doi.org/10.1002/2211-5463.12812catecholamineexercisekidneyrenalaseSp1STAT3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katsuyuki Tokinoya Yasuko Yoshida Takehito Sugasawa Kazuhiro Takekoshi |
spellingShingle |
Katsuyuki Tokinoya Yasuko Yoshida Takehito Sugasawa Kazuhiro Takekoshi Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats FEBS Open Bio catecholamine exercise kidney renalase Sp1 STAT3 |
author_facet |
Katsuyuki Tokinoya Yasuko Yoshida Takehito Sugasawa Kazuhiro Takekoshi |
author_sort |
Katsuyuki Tokinoya |
title |
Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats |
title_short |
Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats |
title_full |
Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats |
title_fullStr |
Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats |
title_full_unstemmed |
Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats |
title_sort |
moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats |
publisher |
Wiley |
series |
FEBS Open Bio |
issn |
2211-5463 |
publishDate |
2020-06-01 |
description |
Renalase is predominantly expressed in the kidney, where it plays a role in catecholamine metabolism and blood pressure regulation. Moderate‐intensity exercise (MEX) has been shown to increase the concentration of renalase in the blood and to reduce renal function in humans. Moreover, such exercise was also reported to increase catecholamine levels. Here, we examined renalase concentration in the blood and renalase expression levels in different organs after MEX in rats. Twelve male Wistar rats were made to run on a treadmill (MEX group) for 60 min at 20 m·min−1, after resting for 15 min. The control group rats were euthanized after resting on the treadmill. Tissue and blood samples were analyzed using western blotting, real‐time RT‐PCR and ELISA. Overall, the concentrations of renalase in the blood were significantly higher in the MEX group than that in the control group. Renalase expression was decreased in the kidney after 60 min of exercise, whereas the expression of renalase mRNA and protein in the extensor digitorum longus and plantaris muscles, respectively, increased after exercise. However, the expression of renalase in the other tissues examined did not change after acute exercise. In conclusion, we report that MEX for 60 min increases both renalase concentration in the blood and its expression in skeletal muscle. |
topic |
catecholamine exercise kidney renalase Sp1 STAT3 |
url |
https://doi.org/10.1002/2211-5463.12812 |
work_keys_str_mv |
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