Genetic and Phylogenetic Analysis of the Mitochondrial Genome in Khuzestan Buffalo
<strong>Objective</strong> A molecular study of the buffalo genetic structure can be effective for better understanding the origin of this animal. Among the molecular markers, mitochondrial genomic sequencing is one of the best and most commonly used methods for genetic classification of...
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Shahid Bahonar University of Kerman
2019-11-01
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Online Access: | https://jab.uk.ac.ir/article_2480_62667471b5252af05d37ebe3ec0e708c.pdf |
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doaj-c2b78f46cd054779b6d5c9f187143c072020-11-25T03:19:29ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002019-11-0111317519010.22103/jab.2019.24802480Genetic and Phylogenetic Analysis of the Mitochondrial Genome in Khuzestan Buffalosima savar0hamidreza seyed abadi1cyrus eidivandi2iman harasi3Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization (AREEO), Karaj, IranAnimal Science Department, Islamic Azad University, Behbahan Unit, Behbahan, Iran.Graduated of Ms. From Islamic Azad University, Behbahan Unit, Behbahan, Iran.<strong>Objective</strong> A molecular study of the buffalo genetic structure can be effective for better understanding the origin of this animal. Among the molecular markers, mitochondrial genomic sequencing is one of the best and most commonly used methods for genetic classification of populations and species close together, studying the possibility of deriving different species from a common ancestor, studying the phylogenic relationship of each species with other species and races, and obtaining solutions for conservation genetic resources. The purpose of this research was to determine the sequence of 12s rRNA and 16s rRNA regions of Khouzestan buffalo. <strong>Materials and methods</strong> For this study, 30 blood samples collected from both sexes of unrelated Khuzestan buffalo. After DNA extraction, the target regions amplified by corresponding specific primers by PCR technique and sequenced after purification. <strong>Results</strong> The results of matching the sequences of 12srRNA and 16srRNA regions of Khuzestan buffalo showed that there was no mutation in this population, which indicates low diversity in Khuzestan buffalo population. According to this study, the 12srRNA and 16srRNA regions are encoding regions and the mutation and diversity are low. The results of the phylogenetic test using UPGMA for both sites showed that the buffaloes of Iran with Indian and Italian buffaloes are in a closely spaced cluster. <strong>Conclusions</strong> Finally, the sequences generated from these regions were the first recorded in the gene bank with MG650115 access code and the name of the Khuzestan buffalo brought to the World Bank Gene, and this race introduced to international associations.https://jab.uk.ac.ir/article_2480_62667471b5252af05d37ebe3ec0e708c.pdfkhuzestan buffalomitochondrial genomesequencing12s rrna16s rrna |
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language |
fas |
format |
Article |
sources |
DOAJ |
author |
sima savar hamidreza seyed abadi cyrus eidivandi iman harasi |
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sima savar hamidreza seyed abadi cyrus eidivandi iman harasi Genetic and Phylogenetic Analysis of the Mitochondrial Genome in Khuzestan Buffalo مجله بیوتکنولوژی کشاورزی khuzestan buffalo mitochondrial genome sequencing 12s rrna 16s rrna |
author_facet |
sima savar hamidreza seyed abadi cyrus eidivandi iman harasi |
author_sort |
sima savar |
title |
Genetic and Phylogenetic Analysis of the Mitochondrial Genome in Khuzestan Buffalo |
title_short |
Genetic and Phylogenetic Analysis of the Mitochondrial Genome in Khuzestan Buffalo |
title_full |
Genetic and Phylogenetic Analysis of the Mitochondrial Genome in Khuzestan Buffalo |
title_fullStr |
Genetic and Phylogenetic Analysis of the Mitochondrial Genome in Khuzestan Buffalo |
title_full_unstemmed |
Genetic and Phylogenetic Analysis of the Mitochondrial Genome in Khuzestan Buffalo |
title_sort |
genetic and phylogenetic analysis of the mitochondrial genome in khuzestan buffalo |
publisher |
Shahid Bahonar University of Kerman |
series |
مجله بیوتکنولوژی کشاورزی |
issn |
2228-6705 2228-6500 |
publishDate |
2019-11-01 |
description |
<strong>Objective</strong>
A molecular study of the buffalo genetic structure can be effective for better understanding the origin of this animal. Among the molecular markers, mitochondrial genomic sequencing is one of the best and most commonly used methods for genetic classification of populations and species close together, studying the possibility of deriving different species from a common ancestor, studying the phylogenic relationship of each species with other species and races, and obtaining solutions for conservation genetic resources. The purpose of this research was to determine the sequence of 12s rRNA and 16s rRNA regions of Khouzestan buffalo.
<strong>Materials and methods</strong>
For this study, 30 blood samples collected from both sexes of unrelated Khuzestan buffalo. After DNA extraction, the target regions amplified by corresponding specific primers by PCR technique and sequenced after purification.
<strong>Results</strong>
The results of matching the sequences of 12srRNA and 16srRNA regions of Khuzestan buffalo showed that there was no mutation in this population, which indicates low diversity in Khuzestan buffalo population. According to this study, the 12srRNA and 16srRNA regions are encoding regions and the mutation and diversity are low. The results of the phylogenetic test using UPGMA for both sites showed that the buffaloes of Iran with Indian and Italian buffaloes are in a closely spaced cluster.
<strong>Conclusions</strong>
Finally, the sequences generated from these regions were the first recorded in the gene bank with MG650115 access code and the name of the Khuzestan buffalo brought to the World Bank Gene, and this race introduced to international associations. |
topic |
khuzestan buffalo mitochondrial genome sequencing 12s rrna 16s rrna |
url |
https://jab.uk.ac.ir/article_2480_62667471b5252af05d37ebe3ec0e708c.pdf |
work_keys_str_mv |
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1724622028718211072 |