Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress

• Main Authors: Qiang Zhang, Caie Wu, Yujun Sun, Tingting Li, Gongjian Fan
• Format: Article
• Language: English
• Published: Institute of Animal Reproduction and Food Research 2019-08-01
• Series: Polish Journal of Food and Nutrition Sciences
• Subjects: morchella esculenta; protein hydrolysate; maillard reaction; oxidative stress; cytoprotective effect
• Online Access: http://www.journalssystem.com/pjfns/The-cytoprotective-effect-of-Morchella-esculenta-protein-hydrolysate-and-it-s-derivative,110134,0,2.html

Morchella protein hydrolysate (MPH) and its glycosylated derivative (G-MPH) may possess the potential as natural antioxidants. However, knowledge about the protective effects of MPH and G-MPH on cellular oxidative damage is limited. This study evaluated whether MPH and G-MPH protected Caco-2 cells from H2O2-induced oxidative injury and explored the potential mechanisms of protection. The results showed that, under H2O2 stress, both MPH and G-MPH significantly increased cell viability, suppressed intracellular ROS and MDA production, increased cellular antioxidant capacity, and activated Nrf2 signaling pathway. More importantly, MPH and G-MPH significantly inhibited the H2O2-induced apoptosis via restoring the loss of mitochondrial membrane potential and regulating the protein expressions of Bax, Bcl-2, and caspase-3. These data indicate that MPH and G-MPH can protect Caco-2 cells against oxidative injury by improving cellular antioxidant responses and inhibiting apoptosis. Therefore, MPH and G-MPH can have a broad application potential as promising ingredients of nutraceutical products or functional foods.