Heuristic Aspect of the Lateral Root Initiation Index: A Case Study of the Role of Nitric Oxide in Root Branching
Premise of the study: Lateral root (LR) initiation (LRI) is a central process in root branching. Based on LR and/or LR primordium densities, it has been shown that nitric oxide (NO) promotes LRI. However, because NO inhibits primary root growth, we hypothesized that NO may have an opposite effect if...
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doaj-c2c35fe2d23c4b4d9b36c114ba5a549f2020-11-24T23:56:27ZengWileyApplications in Plant Sciences2168-04502013-10-01110130002910.3732/apps.1300029Heuristic Aspect of the Lateral Root Initiation Index: A Case Study of the Role of Nitric Oxide in Root BranchingVeronica Lira-Ruan0Selene Napsucialy Mendivil1Joseph G. Dubrovsky2Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250 Cuernavaca, Morelos, México; Laboratorio de Fisiología y Desarrollo Vegetal, Facultad de Ciencias, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, MéxicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250 Cuernavaca, Morelos, MéxicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250 Cuernavaca, Morelos, MéxicoPremise of the study: Lateral root (LR) initiation (LRI) is a central process in root branching. Based on LR and/or LR primordium densities, it has been shown that nitric oxide (NO) promotes LRI. However, because NO inhibits primary root growth, we hypothesized that NO may have an opposite effect if the analysis is performed on a cellular basis. Using a previously proposed parameter, the LRI index (which measures how many LRI events take place along a root portion equivalent to the length of a single file of 100 cortical cells of average length), we addressed this hypothesis and illustrate here that the LRI index provides a researcher with a tool to uncover hidden but important information about root initiation. Methods and Results: <i>Arabidopsis thaliana</i> roots were treated with an NO donor (sodium nitroprusside [SNP]) and/or an NO scavenger (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-l-oxyl-3-oxide [cPTIO]). LRI was analyzed separately in the root portions formed before and during the treatment. In the latter, SNP caused root growth inhibition and an increase in the LR density accompanied by a decrease in LRI index, indicating overall inhibitory outcome of the NO donor on branching. The inhibitory effect of SNP was reversed by cPTIO, showing the NO-specific action of SNP on LRI. Conclusions: Analysis of the LRI index permits the discovery of otherwise unknown modes of action of a substance on the root system formation. NO has a dual action on root branching, slightly promoting it in the root portion formed before the treatment and strongly inhibiting it in the root portion formed during the treatment.http://www.bioone.org/doi/full/10.3732/apps.1300029lateral root initiationnitric oxideroot branchingroot system architecture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Veronica Lira-Ruan Selene Napsucialy Mendivil Joseph G. Dubrovsky |
spellingShingle |
Veronica Lira-Ruan Selene Napsucialy Mendivil Joseph G. Dubrovsky Heuristic Aspect of the Lateral Root Initiation Index: A Case Study of the Role of Nitric Oxide in Root Branching Applications in Plant Sciences lateral root initiation nitric oxide root branching root system architecture |
author_facet |
Veronica Lira-Ruan Selene Napsucialy Mendivil Joseph G. Dubrovsky |
author_sort |
Veronica Lira-Ruan |
title |
Heuristic Aspect of the Lateral Root Initiation Index: A Case Study of the Role of Nitric Oxide in Root Branching |
title_short |
Heuristic Aspect of the Lateral Root Initiation Index: A Case Study of the Role of Nitric Oxide in Root Branching |
title_full |
Heuristic Aspect of the Lateral Root Initiation Index: A Case Study of the Role of Nitric Oxide in Root Branching |
title_fullStr |
Heuristic Aspect of the Lateral Root Initiation Index: A Case Study of the Role of Nitric Oxide in Root Branching |
title_full_unstemmed |
Heuristic Aspect of the Lateral Root Initiation Index: A Case Study of the Role of Nitric Oxide in Root Branching |
title_sort |
heuristic aspect of the lateral root initiation index: a case study of the role of nitric oxide in root branching |
publisher |
Wiley |
series |
Applications in Plant Sciences |
issn |
2168-0450 |
publishDate |
2013-10-01 |
description |
Premise of the study: Lateral root (LR) initiation (LRI) is a central process in root branching. Based on LR and/or LR primordium densities, it has been shown that nitric oxide (NO) promotes LRI. However, because NO inhibits primary root growth, we hypothesized that NO may have an opposite effect if the analysis is performed on a cellular basis. Using a previously proposed parameter, the LRI index (which measures how many LRI events take place along a root portion equivalent to the length of a single file of 100 cortical cells of average length), we addressed this hypothesis and illustrate here that the LRI index provides a researcher with a tool to uncover hidden but important information about root initiation.
Methods and Results: <i>Arabidopsis thaliana</i> roots were treated with an NO donor (sodium nitroprusside [SNP]) and/or an NO scavenger (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-l-oxyl-3-oxide [cPTIO]). LRI was analyzed separately in the root portions formed before and during the treatment. In the latter, SNP caused root growth inhibition and an increase in the LR density accompanied by a decrease in LRI index, indicating overall inhibitory outcome of the NO donor on branching. The inhibitory effect of SNP was reversed by cPTIO, showing the NO-specific action of SNP on LRI.
Conclusions: Analysis of the LRI index permits the discovery of otherwise unknown modes of action of a substance on the root system formation. NO has a dual action on root branching, slightly promoting it in the root portion formed before the treatment and strongly inhibiting it in the root portion formed during the treatment. |
topic |
lateral root initiation nitric oxide root branching root system architecture |
url |
http://www.bioone.org/doi/full/10.3732/apps.1300029 |
work_keys_str_mv |
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