Synergistic Effects of Hyperosmolarity and Transforming Growth Factor Beta (TGF-β) on Chondrogenic Differentiation and Reducing Neotissue Ossification
Background: Mesenchymal stem cells (MSCs), are multipotent stem cells that their differentiation to chondrocytes is considered in recent years. Platelet-rich plasma (PRP), because of rich of several growth factors, and also hyperosmolarity, because of stimulating differentiation culture medium to or...
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doaj-c2cf8a4e6b02444fa29557e8ed51723c2020-11-25T01:08:57ZfasVesnu Publications مجله دانشکده پزشکی اصفهان1027-75951735-854X2019-04-013751510110710.22122/jims.v37i515.110873316Synergistic Effects of Hyperosmolarity and Transforming Growth Factor Beta (TGF-β) on Chondrogenic Differentiation and Reducing Neotissue OssificationElham Konar0Saeed Reza Khatami1Hana Hanaee-Ahvaz2Mohamad Shfiee3Mohamad Reza Hajari4PhD Student, Department of Biology, School of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranAssociate Professor, Department of Biology, School of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranAssistant Professor, Department of Stem Cells, Stem Cell Technology Research Center, Tehran, IranAssistant Professor, Department of Biology, School of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranAssistant Professor, Department of Biology, School of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranBackground: Mesenchymal stem cells (MSCs), are multipotent stem cells that their differentiation to chondrocytes is considered in recent years. Platelet-rich plasma (PRP), because of rich of several growth factors, and also hyperosmolarity, because of stimulating differentiation culture medium to original medium of chondrocytes, can affect this differentiation. Considering hypertrophy and ossification as resulted adverse factors, this study aimed to achieve an optimal medium for chondrogenic differentiation of human MSCs. Methods: MSCs were extracted from adipose tissue, and differentiated to chondrocyte line. The effect of base medium containing transforming growth factor beta (TGF-β), PRP, hyperosmolarity, base + hyperosmolarity and PRP + hyperosmolarity on chondrogenic differentiation was assessed using special staining methods such as Alcian blue, hematoxylin and eosin (H&E), and collagen type II and X immunocytochemistry, measurement of biochemical indexes including alkaline phosphatase (ALP) activity and deposit of calcium, and study of expression of Col2, Smad2, Sox9, and Aggrecan genes using real-time polymerase chain reaction (RT-PCR). Findings: Human adipose tissue derived MSCs, with differentiation ability to adipocyte and osteocyte lines, were presented characterizations of chondrocytes in all differentiation groups. In base + hyperosmolarity group, ALP activity and deposit of calcium were less than other groups. Conclusion: Base + hyperosmolarity differentiation group can be a better culture medium for chondrogenic differentiation.http://jims.mui.ac.ir/index.php/jims/article/view/11087Mesenchymal stem cellsCell differentiationPlatelet-Rich PlasmaChondrocytes |
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DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Elham Konar Saeed Reza Khatami Hana Hanaee-Ahvaz Mohamad Shfiee Mohamad Reza Hajari |
spellingShingle |
Elham Konar Saeed Reza Khatami Hana Hanaee-Ahvaz Mohamad Shfiee Mohamad Reza Hajari Synergistic Effects of Hyperosmolarity and Transforming Growth Factor Beta (TGF-β) on Chondrogenic Differentiation and Reducing Neotissue Ossification مجله دانشکده پزشکی اصفهان Mesenchymal stem cells Cell differentiation Platelet-Rich Plasma Chondrocytes |
author_facet |
Elham Konar Saeed Reza Khatami Hana Hanaee-Ahvaz Mohamad Shfiee Mohamad Reza Hajari |
author_sort |
Elham Konar |
title |
Synergistic Effects of Hyperosmolarity and Transforming Growth Factor Beta (TGF-β) on Chondrogenic Differentiation and Reducing Neotissue Ossification |
title_short |
Synergistic Effects of Hyperosmolarity and Transforming Growth Factor Beta (TGF-β) on Chondrogenic Differentiation and Reducing Neotissue Ossification |
title_full |
Synergistic Effects of Hyperosmolarity and Transforming Growth Factor Beta (TGF-β) on Chondrogenic Differentiation and Reducing Neotissue Ossification |
title_fullStr |
Synergistic Effects of Hyperosmolarity and Transforming Growth Factor Beta (TGF-β) on Chondrogenic Differentiation and Reducing Neotissue Ossification |
title_full_unstemmed |
Synergistic Effects of Hyperosmolarity and Transforming Growth Factor Beta (TGF-β) on Chondrogenic Differentiation and Reducing Neotissue Ossification |
title_sort |
synergistic effects of hyperosmolarity and transforming growth factor beta (tgf-β) on chondrogenic differentiation and reducing neotissue ossification |
publisher |
Vesnu Publications |
series |
مجله دانشکده پزشکی اصفهان |
issn |
1027-7595 1735-854X |
publishDate |
2019-04-01 |
description |
Background: Mesenchymal stem cells (MSCs), are multipotent stem cells that their differentiation to chondrocytes is considered in recent years. Platelet-rich plasma (PRP), because of rich of several growth factors, and also hyperosmolarity, because of stimulating differentiation culture medium to original medium of chondrocytes, can affect this differentiation. Considering hypertrophy and ossification as resulted adverse factors, this study aimed to achieve an optimal medium for chondrogenic differentiation of human MSCs.
Methods: MSCs were extracted from adipose tissue, and differentiated to chondrocyte line. The effect of base medium containing transforming growth factor beta (TGF-β), PRP, hyperosmolarity, base + hyperosmolarity and PRP + hyperosmolarity on chondrogenic differentiation was assessed using special staining methods such as Alcian blue, hematoxylin and eosin (H&E), and collagen type II and X immunocytochemistry, measurement of biochemical indexes including alkaline phosphatase (ALP) activity and deposit of calcium, and study of expression of Col2, Smad2, Sox9, and Aggrecan genes using real-time polymerase chain reaction (RT-PCR).
Findings: Human adipose tissue derived MSCs, with differentiation ability to adipocyte and osteocyte lines, were presented characterizations of chondrocytes in all differentiation groups. In base + hyperosmolarity group, ALP activity and deposit of calcium were less than other groups.
Conclusion: Base + hyperosmolarity differentiation group can be a better culture medium for chondrogenic differentiation. |
topic |
Mesenchymal stem cells Cell differentiation Platelet-Rich Plasma Chondrocytes |
url |
http://jims.mui.ac.ir/index.php/jims/article/view/11087 |
work_keys_str_mv |
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