Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol

Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol

Abstract Ortho-phenylphenol (OPP) is a fungicide contained in agro-industrial effluents produced by fruit-packaging plants. Within the frame of developing bio-strategies to detoxify these effluents, an OPP-degrading Sphingomonas haloaromaticamans strain was isolated. Proteins/genes with a putative c...

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Main Authors: Chiara Perruchon, Sotirios Vasileiadis, Constantina Rousidou, Evangelia S. Papadopoulou, Georgia Tanou, Martina Samiotaki, Constantinos Garagounis, Athanasios Molassiotis, Kalliope K. Papadopoulou, Dimitrios G. Karpouzas
Format: Article
Language:English
Published: Nature Publishing Group 2017-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-06727-6
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spelling doaj-c2dcdf92add349889a4915af7b622dac2020-12-08T03:06:38ZengNature Publishing GroupScientific Reports2045-23222017-07-017111410.1038/s41598-017-06727-6Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenolChiara Perruchon0Sotirios Vasileiadis1Constantina Rousidou2Evangelia S. Papadopoulou3Georgia Tanou4Martina Samiotaki5Constantinos Garagounis6Athanasios Molassiotis7Kalliope K. Papadopoulou8Dimitrios G. Karpouzas9Department of Biochemistry and Biotechnology, University of Thessaly, Laboratory of Plant and Environmental BiotechnologyUniversity of South Australia, Future Industries InstituteDepartment of Biochemistry and Biotechnology, University of Thessaly, Laboratory of Plant and Environmental BiotechnologyDepartment of Biochemistry and Biotechnology, University of Thessaly, Laboratory of Plant and Environmental BiotechnologyAristotle University of Thessaloniki, School of AgricultureBiomedical Sciences Research Center “Alexander Fleming”Department of Biochemistry and Biotechnology, University of Thessaly, Laboratory of Plant and Environmental BiotechnologyAristotle University of Thessaloniki, School of AgricultureDepartment of Biochemistry and Biotechnology, University of Thessaly, Laboratory of Plant and Environmental BiotechnologyDepartment of Biochemistry and Biotechnology, University of Thessaly, Laboratory of Plant and Environmental BiotechnologyAbstract Ortho-phenylphenol (OPP) is a fungicide contained in agro-industrial effluents produced by fruit-packaging plants. Within the frame of developing bio-strategies to detoxify these effluents, an OPP-degrading Sphingomonas haloaromaticamans strain was isolated. Proteins/genes with a putative catabolic role and bacterium adaptation mechanisms during OPP degradation were identified via genomic and proteomic analysis. Transcription analysis of all putative catabolic genes established their role in the metabolism of OPP. The formation of key transformation products was verified by chromatographic analysis. Genomic analysis identified two orthologous operons encoding the ortho-cleavage of benzoic acid (BA) (ben/cat). The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon (opp) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon (bph). Proteomics identified 13 up-regulated catabolic proteins when S. haloaromaticamans was growing on OPP and/or BA. Transcription analysis verified the key role of the catabolic operons located in the 92-kb scaffold, and flanked by transposases, on the transformation of OPP by S. haloaromaticamans. A flavin-dependent monoxygenase (OppA1), one of the most up-regulated proteins in the OPP-growing cells, was isolated via heterologous expression and its catabolic activity was verified in vitro.https://doi.org/10.1038/s41598-017-06727-6
collection DOAJ
language English
format Article
sources DOAJ
author Chiara Perruchon
Sotirios Vasileiadis
Constantina Rousidou
Evangelia S. Papadopoulou
Georgia Tanou
Martina Samiotaki
Constantinos Garagounis
Athanasios Molassiotis
Kalliope K. Papadopoulou
Dimitrios G. Karpouzas
spellingShingle Chiara Perruchon
Sotirios Vasileiadis
Constantina Rousidou
Evangelia S. Papadopoulou
Georgia Tanou
Martina Samiotaki
Constantinos Garagounis
Athanasios Molassiotis
Kalliope K. Papadopoulou
Dimitrios G. Karpouzas
Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol
Scientific Reports
author_facet Chiara Perruchon
Sotirios Vasileiadis
Constantina Rousidou
Evangelia S. Papadopoulou
Georgia Tanou
Martina Samiotaki
Constantinos Garagounis
Athanasios Molassiotis
Kalliope K. Papadopoulou
Dimitrios G. Karpouzas
author_sort Chiara Perruchon
title Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol
title_short Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol
title_full Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol
title_fullStr Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol
title_full_unstemmed Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol
title_sort metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a sphingomonas haloaromaticamans strain degrading ortho-phenylphenol
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2017-07-01
description Abstract Ortho-phenylphenol (OPP) is a fungicide contained in agro-industrial effluents produced by fruit-packaging plants. Within the frame of developing bio-strategies to detoxify these effluents, an OPP-degrading Sphingomonas haloaromaticamans strain was isolated. Proteins/genes with a putative catabolic role and bacterium adaptation mechanisms during OPP degradation were identified via genomic and proteomic analysis. Transcription analysis of all putative catabolic genes established their role in the metabolism of OPP. The formation of key transformation products was verified by chromatographic analysis. Genomic analysis identified two orthologous operons encoding the ortho-cleavage of benzoic acid (BA) (ben/cat). The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon (opp) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon (bph). Proteomics identified 13 up-regulated catabolic proteins when S. haloaromaticamans was growing on OPP and/or BA. Transcription analysis verified the key role of the catabolic operons located in the 92-kb scaffold, and flanked by transposases, on the transformation of OPP by S. haloaromaticamans. A flavin-dependent monoxygenase (OppA1), one of the most up-regulated proteins in the OPP-growing cells, was isolated via heterologous expression and its catabolic activity was verified in vitro.
url https://doi.org/10.1038/s41598-017-06727-6
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