GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine max
Abstract Background Brassinosteroids (BRs) play a crucial role in plant vegetative growth and reproductive development. The transcription factors BZR1 and BES1/BZR2 are well characterized as downstream regulators of the BR signaling pathway in Arabidopsis and rice. Soybean contains four BZR1-like pr...
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doaj-c2ea867e04bc400b855b66a32f62f6a72020-11-25T03:03:58ZengBMCBMC Plant Biology1471-22292019-02-0119111510.1186/s12870-019-1677-2GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine maxLi Song0Wei Chen1Biao Wang2Qiu-Ming Yao3Babu Valliyodan4Ming-Yi Bai5Ming-Zhe Zhao6Heng Ye7Zhi-Yong Wang8Henry T. Nguyen9Joint International Research Laboratory of Agriculture and Agri-Product Safety, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou UniversityDivision of Plant Sciences, University of MissouriSchool of Agriculture and Biology, Shanghai Jiao Tong UniversityDepartment of Computer Science, Informatics Institute, and Christopher S. Bond Life, Sciences Center, University of MissouriDivision of Plant Sciences, University of MissouriDepartment of Plant Biology, Carnegie Institution for ScienceDivision of Plant Sciences, University of MissouriDivision of Plant Sciences, University of MissouriDepartment of Plant Biology, Carnegie Institution for ScienceDivision of Plant Sciences, University of MissouriAbstract Background Brassinosteroids (BRs) play a crucial role in plant vegetative growth and reproductive development. The transcription factors BZR1 and BES1/BZR2 are well characterized as downstream regulators of the BR signaling pathway in Arabidopsis and rice. Soybean contains four BZR1-like proteins (GmBZLs), and it was reported that GmBZL2 plays a conserved role in BR signaling regulation. However, the roles of other GmBZLs have not been thoroughly studied, and the targets of GmBZLs in soybean remain unclear. Results In this study, we first characterized GmBZL3 in soybean from gene expression patterns, conserved domains in coding sequences, and genomic replication times of four GmBZL orthologous. The results indicated that GmBZL3 might play conserved roles during soybean development. The overexpression of GmBZL3 P219L in the Arabidopsis BR-insensitive mutant bri1–5 partially rescued the phenotypic defects including BR-insensitivity, which provides further evidence that GmBZL3 functions are conserved between soybean and the homologous Arabidopsis genes. In addition, the identification of the GmBZL3 target genes through ChIP-seq technology revealed that BR has broad roles in soybean and regulates multiple pathways, including other hormone signaling, disease-related, and immunity response pathways. Moreover, the BR-regulated GmBZL3 target genes were further identified, and the results demonstrate that GmBZL3 is a major transcription factor responsible for BR-regulated gene expression and soybean growth. A comparison of GmBZL3 and AtBZR1/BES1 targets demonstrated that GmBZL3 might play conserved as well as specific roles in the soybean BR signaling network. Finally, the identification of two natural soybean varieties of the GmBZL3 mutantion by SNP analysis could facilitate the understanding of gene function during soybean development in the future. Conclusions We illustrate here that GmBZL3 orchestrates a genome-wide transcriptional response that underlies BR-mediated soybean early vegetative growth, and our results support that BRs play crucial regulatory roles in soybean morphology and gene expression levels.http://link.springer.com/article/10.1186/s12870-019-1677-2SoybeanGmBZL3BrassinosteroidChIP-seqHormone crosstalkSNP |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Song Wei Chen Biao Wang Qiu-Ming Yao Babu Valliyodan Ming-Yi Bai Ming-Zhe Zhao Heng Ye Zhi-Yong Wang Henry T. Nguyen |
spellingShingle |
Li Song Wei Chen Biao Wang Qiu-Ming Yao Babu Valliyodan Ming-Yi Bai Ming-Zhe Zhao Heng Ye Zhi-Yong Wang Henry T. Nguyen GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine max BMC Plant Biology Soybean GmBZL3 Brassinosteroid ChIP-seq Hormone crosstalk SNP |
author_facet |
Li Song Wei Chen Biao Wang Qiu-Ming Yao Babu Valliyodan Ming-Yi Bai Ming-Zhe Zhao Heng Ye Zhi-Yong Wang Henry T. Nguyen |
author_sort |
Li Song |
title |
GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine max |
title_short |
GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine max |
title_full |
GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine max |
title_fullStr |
GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine max |
title_full_unstemmed |
GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine max |
title_sort |
gmbzl3 acts as a major br signaling regulator through crosstalk with multiple pathways in glycine max |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2019-02-01 |
description |
Abstract Background Brassinosteroids (BRs) play a crucial role in plant vegetative growth and reproductive development. The transcription factors BZR1 and BES1/BZR2 are well characterized as downstream regulators of the BR signaling pathway in Arabidopsis and rice. Soybean contains four BZR1-like proteins (GmBZLs), and it was reported that GmBZL2 plays a conserved role in BR signaling regulation. However, the roles of other GmBZLs have not been thoroughly studied, and the targets of GmBZLs in soybean remain unclear. Results In this study, we first characterized GmBZL3 in soybean from gene expression patterns, conserved domains in coding sequences, and genomic replication times of four GmBZL orthologous. The results indicated that GmBZL3 might play conserved roles during soybean development. The overexpression of GmBZL3 P219L in the Arabidopsis BR-insensitive mutant bri1–5 partially rescued the phenotypic defects including BR-insensitivity, which provides further evidence that GmBZL3 functions are conserved between soybean and the homologous Arabidopsis genes. In addition, the identification of the GmBZL3 target genes through ChIP-seq technology revealed that BR has broad roles in soybean and regulates multiple pathways, including other hormone signaling, disease-related, and immunity response pathways. Moreover, the BR-regulated GmBZL3 target genes were further identified, and the results demonstrate that GmBZL3 is a major transcription factor responsible for BR-regulated gene expression and soybean growth. A comparison of GmBZL3 and AtBZR1/BES1 targets demonstrated that GmBZL3 might play conserved as well as specific roles in the soybean BR signaling network. Finally, the identification of two natural soybean varieties of the GmBZL3 mutantion by SNP analysis could facilitate the understanding of gene function during soybean development in the future. Conclusions We illustrate here that GmBZL3 orchestrates a genome-wide transcriptional response that underlies BR-mediated soybean early vegetative growth, and our results support that BRs play crucial regulatory roles in soybean morphology and gene expression levels. |
topic |
Soybean GmBZL3 Brassinosteroid ChIP-seq Hormone crosstalk SNP |
url |
http://link.springer.com/article/10.1186/s12870-019-1677-2 |
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