Production of monoclonal antibody to MSP-2 and evaluation of different domains of the molecule

• Main Authors: afra Khosravi, eghbaleh Asadollahi, poul Hommel
• Format: Article
• Language: fas
• Published: Lorestan University of Medical Science 2009-04-01
• Series: Yafteh
• Subjects: MSP-2; Immunization; Plasmodium Falciparum; Malaria
• Online Access: http://yafte.lums.ac.ir/browse.php?a_code=A-10-1-111&slc_lang=en&sid=1

Background: Plasmodiom falciparum MSP-2 is taken into accout as one of the candidates of vaccine agaist malaria and various studies indicated immunizing effect of different domains of the molecule. Monoclonal antibody against MSP-2 is a confident and definite method to confirm this Immunization. Materials and Methods: P. falciparum Parasite was Prepared in a Culture in CludingRPMI1640 and Schizontantingens extracted and injected into the mice. Then B Lyphocytes of immunized mice were extracted from their spleen and the fusion was Performed using NS-1 and monoclonal antibody to MSP-2 was Produced. Results: Anumber of monoclonal antibody to MSP-2 weres Produced which confirmed efficiency and Immunizing effects of various domains of MSP-2 molecule.