Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections
The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes....
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Sociedade Brasileira de Microbiologia
2014-12-01
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doaj-c2f038765b5c44d7a45c157adc99d4cf2020-11-24T21:27:52ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-44052014-12-0145414011407S1517-83822014000400034Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infectionsMariana Fávero Bonesso0Silvio Alencar Marques1Carlos Henrique Camargo2Carlos Magno Castelo Branco Fortaleza3Maria de Lourdes Ribeiro de Souza da Cunha4Universidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Estadual PaulistaThe aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medical School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types detected in the seven mecA-positive S. aureus strains were type Ia in one, type II in three, and type IV in three. The PVL gene was detected in 10 (15.1%) in sensitive strains. Pulsed field gel electrophoresis revealed non-clonal diversity among the isolates. The risk factors associated with MRSA acquisition in this study were previous ciprofloxacin use and working in a healthcare environment. The risk factors indicate plausible routes of CA-MRSA transmission among the subjects studied.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400034&lng=en&tlng=enStaphylococcus aureusresistanceskin infectionsvirulenceepidemiology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariana Fávero Bonesso Silvio Alencar Marques Carlos Henrique Camargo Carlos Magno Castelo Branco Fortaleza Maria de Lourdes Ribeiro de Souza da Cunha |
spellingShingle |
Mariana Fávero Bonesso Silvio Alencar Marques Carlos Henrique Camargo Carlos Magno Castelo Branco Fortaleza Maria de Lourdes Ribeiro de Souza da Cunha Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections Brazilian Journal of Microbiology Staphylococcus aureus resistance skin infections virulence epidemiology |
author_facet |
Mariana Fávero Bonesso Silvio Alencar Marques Carlos Henrique Camargo Carlos Magno Castelo Branco Fortaleza Maria de Lourdes Ribeiro de Souza da Cunha |
author_sort |
Mariana Fávero Bonesso |
title |
Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections |
title_short |
Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections |
title_full |
Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections |
title_fullStr |
Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections |
title_full_unstemmed |
Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections |
title_sort |
community-associated methicillin-resistant staphylococcus aureus in non-outbreak skin infections |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
publishDate |
2014-12-01 |
description |
The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medical School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types detected in the seven mecA-positive S. aureus strains were type Ia in one, type II in three, and type IV in three. The PVL gene was detected in 10 (15.1%) in sensitive strains. Pulsed field gel electrophoresis revealed non-clonal diversity among the isolates. The risk factors associated with MRSA acquisition in this study were previous ciprofloxacin use and working in a healthcare environment. The risk factors indicate plausible routes of CA-MRSA transmission among the subjects studied. |
topic |
Staphylococcus aureus resistance skin infections virulence epidemiology |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400034&lng=en&tlng=en |
work_keys_str_mv |
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