Comparison of Resistant and Susceptible Strains of Trichomons vaginalis to Metronidazole Using PCR Method
Background: Metronidazole is drug of choice recommended by WHO for treatment of trichomoniasis, however, some reports claims drug resistance in Trichomonas vaginalis isolates recently. The objective of this study was to determine the minimum lethal concentration (MLC) of metronidazole in resistant a...
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Tehran University of Medical Sciences
2012-09-01
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doaj-c2f1cff8eed547ae975dcf1c550f78742021-04-02T11:02:50ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2012-09-0173Comparison of Resistant and Susceptible Strains of Trichomons vaginalis to Metronidazole Using PCR MethodS Rabiee0A Bazmani1M Fallah2M Matini3Dept. of Obstetrics and Gynecology, Fatemieh Women Hospital, Hamadan University of Medical Science, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDept. of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Science, Hamadan, IranDept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Science, Tehran, IranBackground: Metronidazole is drug of choice recommended by WHO for treatment of trichomoniasis, however, some reports claims drug resistance in Trichomonas vaginalis isolates recently. The objective of this study was to determine the minimum lethal concentration (MLC) of metronidazole in resistant and sensitive strains, as well as genetic patterns of these stains by PCR method. Methods: From February 2006 to March 2007, in a cross sectional study, clinical and wet mount examination of vaginal smear along with culture were performed on 683 women attending to public and private outpatient clinics in Hamadan. Trichomoniasis marked based on major clinical symptoms. Diagnosis confirmed using wet mount microscopically and culture in Diamond medium. A serial concentration of metronidazole was provided and all isolated Trichomonas strains (resistant and sensitive) tested by standard method. Finally, all sensitive and resistant strains examined by PCR technique. Results: Only 15/683, (2.2%) of patients clinically diagnosed trichomonal vaginitis were positive for T. vaginalis by wet smear and culture. The minimum lethal concentration (MLC) for clinically sensitive isolates was 25 µg/ml; however, this concentration for resistant isolates was 200 µg/ml after 24 h and 100 µg/ml after 50 h. The results of PCR examination of DNA from sensitive and resistant isolates had same pattern. The lanes appeared by two primers were 98 bp and 261 bp for both clinically sensitive and resistant strains. Conclusion: Resistance to metronidazole in T. vaginalis has not relation to genetic variations and might be related to some physiologic pathways of organism. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/248Trichomonas vaginalisMetronidazoleResistancePCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S Rabiee A Bazmani M Fallah M Matini |
spellingShingle |
S Rabiee A Bazmani M Fallah M Matini Comparison of Resistant and Susceptible Strains of Trichomons vaginalis to Metronidazole Using PCR Method Iranian Journal of Parasitology Trichomonas vaginalis Metronidazole Resistance PCR |
author_facet |
S Rabiee A Bazmani M Fallah M Matini |
author_sort |
S Rabiee |
title |
Comparison of Resistant and Susceptible Strains of Trichomons vaginalis to Metronidazole Using PCR Method |
title_short |
Comparison of Resistant and Susceptible Strains of Trichomons vaginalis to Metronidazole Using PCR Method |
title_full |
Comparison of Resistant and Susceptible Strains of Trichomons vaginalis to Metronidazole Using PCR Method |
title_fullStr |
Comparison of Resistant and Susceptible Strains of Trichomons vaginalis to Metronidazole Using PCR Method |
title_full_unstemmed |
Comparison of Resistant and Susceptible Strains of Trichomons vaginalis to Metronidazole Using PCR Method |
title_sort |
comparison of resistant and susceptible strains of trichomons vaginalis to metronidazole using pcr method |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2012-09-01 |
description |
Background: Metronidazole is drug of choice recommended by WHO for treatment of trichomoniasis, however, some reports claims drug resistance in Trichomonas vaginalis isolates recently. The objective of this study was to determine the minimum lethal concentration (MLC) of metronidazole in resistant and sensitive strains, as well as genetic patterns of these stains by PCR method.
Methods: From February 2006 to March 2007, in a cross sectional study, clinical and wet mount examination of vaginal smear along with culture were performed on 683 women attending to public and private outpatient clinics in Hamadan. Trichomoniasis marked based on major clinical symptoms. Diagnosis confirmed using wet mount microscopically and culture in Diamond medium. A serial concentration of metronidazole was provided and all isolated Trichomonas strains (resistant and sensitive) tested by standard method. Finally, all sensitive and resistant strains examined by PCR technique.
Results: Only 15/683, (2.2%) of patients clinically diagnosed trichomonal vaginitis were positive for T. vaginalis by wet smear and culture. The minimum lethal concentration (MLC) for clinically sensitive isolates was 25 µg/ml; however, this concentration for resistant isolates was 200 µg/ml after 24 h and 100 µg/ml after 50 h. The results of PCR examination of DNA from sensitive and resistant isolates had same pattern. The lanes appeared by two primers were 98 bp and 261 bp for both clinically sensitive and resistant strains.
Conclusion: Resistance to metronidazole in T. vaginalis has not relation to genetic variations and might be related to some physiologic pathways of organism.
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topic |
Trichomonas vaginalis Metronidazole Resistance PCR |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/248 |
work_keys_str_mv |
AT srabiee comparisonofresistantandsusceptiblestrainsoftrichomonsvaginalistometronidazoleusingpcrmethod AT abazmani comparisonofresistantandsusceptiblestrainsoftrichomonsvaginalistometronidazoleusingpcrmethod AT mfallah comparisonofresistantandsusceptiblestrainsoftrichomonsvaginalistometronidazoleusingpcrmethod AT mmatini comparisonofresistantandsusceptiblestrainsoftrichomonsvaginalistometronidazoleusingpcrmethod |
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