Incorporation of cis-9,trans-11 or trans-10,cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men

• Main Authors: Graham C. Burdge, Berit Lupoli, Jennifer J. Russell, Sabine Tricon, Samantha Kew, Tapati Banerjee, Kevin J. Shingfield, David E. Beever, Robert F. Grimble, Christine M. Williams, Parveen Yaqoob, Philip C. Calder
• Format: Article
• Language: English
• Published: Elsevier 2004-04-01
• Series: Journal of Lipid Research
• Subjects: phosphatidylcholine; cholesteryl ester; nonesterified fatty acids; leukocyte; human
• Online Access: http://www.sciencedirect.com/science/article/pii/S0022227520318587

This study investigated the incorporation of cis-9,trans-11 conjugated linoleic acid (c9,t11 CLA) and trans-10,cis-12-CLA (t10,c12 CLA) into plasma and peripheral blood mononuclear cell (PBMC) lipids when consumed as supplements highly enriched in these isomers. Healthy men (n = 49, age 31 ± 8 years) consumed one, two, and four capsules containing ∼600 mg of either c9,t11 CLA or t10,c12 CLA per capsule for sequential 8 week periods followed by a 6 week washout before consuming the alternative isomer. Both isomers were incorporated in a dose-dependent manner into plasma phosphatidylcholine (PC) (c9,t11 CLA r = 0.779, t10,c12 CLA r = 0.738; P < 0.0001) and cholesteryl ester (CE) (c9,t11 CLA r = 0.706, t10,c12 CLA r = 0.788; P < 0.0001). Only t10,c12 CLA was enriched in plasma nonesterified fatty acids. Both c9,t11 CLA and t10,c12 CLA were incorporated linearly into PBMC total lipids (r = 0.285 and r = 0.273, respectively; P < 0.0005). The highest concentrations of c9,t11 CLA and t10,c12 CLA in PBMC lipids were 3- to 4-fold lower than those in plasma PC and CE.These data suggest that the level of intake is a major determinant of plasma and PBMC CLA content, although PBMCs appear to incorporate both CLA isomers less readily.