Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic <i>Pseudomonas</i> sp. B14-6

Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic <i>Pseudomonas</i> sp. B14-6

Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium <i>Pseudomonas</i> sp. B14-6 revealed two PHA-productio...

Full description

Bibliographic Details
Main Authors: Tae-Rim Choi, Ye-Lim Park, Hun-Suk Song, Sun Mi Lee, Sol Lee Park, Hye Soo Lee, Hyun-Joong Kim, Shashi Kant Bhatia, Ranjit Gurav, Kwon-Young Choi, Yoo Kyung Lee, Yung-Hun Yang
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Polymers
Subjects:
<i>Pseudomonas</i> strain
polyhydroxyalkanoate
fructose syrup
fermentation
Online Access:https://www.mdpi.com/2073-4360/13/9/1398
id doaj-c311799de67a47c5a2605ec2f0033cda
record_format Article
spelling doaj-c311799de67a47c5a2605ec2f0033cda2021-04-26T23:02:03ZengMDPI AGPolymers2073-43602021-04-01131398139810.3390/polym13091398Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic <i>Pseudomonas</i> sp. B14-6Tae-Rim Choi0Ye-Lim Park1Hun-Suk Song2Sun Mi Lee3Sol Lee Park4Hye Soo Lee5Hyun-Joong Kim6Shashi Kant Bhatia7Ranjit Gurav8Kwon-Young Choi9Yoo Kyung Lee10Yung-Hun Yang11Department of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaDepartment of Environmental and Safety Engineering, College of Engineering, Ajou University, Suwon-si 16499, Gyeonggi-do, KoreaPolar Research Institute, Incheon 21990, KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, KoreaArctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium <i>Pseudomonas</i> sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes (<i>phaC</i>). <i>Pseudomonas</i> sp. B14-6 produced poly(6% 3-hydroxybutyrate-<i>co</i>-94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl-<i>co</i>-mcl PHA determined a glass transition temperature (T<sub>g</sub>) of 15.3 °C, melting temperature of 112.8 °C, crystallization temperature of 86.8 °C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 × 10<sup>4</sup>, weight average molecular weight of 9.1 × 10<sup>4</sup>, and polydispersity index value of 2.5. Overall, the novel <i>Pseudomonas</i> sp. B14-6 produced a polymer with high medium-chain-length content, low T<sub>g</sub>, and low crystallinity, indicating its potential use in medical applications.https://www.mdpi.com/2073-4360/13/9/1398<i>Pseudomonas</i> strainpolyhydroxyalkanoatefructose syrupfermentation
collection DOAJ
language English
format Article
sources DOAJ
author Tae-Rim Choi
Ye-Lim Park
Hun-Suk Song
Sun Mi Lee
Sol Lee Park
Hye Soo Lee
Hyun-Joong Kim
Shashi Kant Bhatia
Ranjit Gurav
Kwon-Young Choi
Yoo Kyung Lee
Yung-Hun Yang
spellingShingle Tae-Rim Choi
Ye-Lim Park
Hun-Suk Song
Sun Mi Lee
Sol Lee Park
Hye Soo Lee
Hyun-Joong Kim
Shashi Kant Bhatia
Ranjit Gurav
Kwon-Young Choi
Yoo Kyung Lee
Yung-Hun Yang
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic <i>Pseudomonas</i> sp. B14-6
Polymers
<i>Pseudomonas</i> strain
polyhydroxyalkanoate
fructose syrup
fermentation
author_facet Tae-Rim Choi
Ye-Lim Park
Hun-Suk Song
Sun Mi Lee
Sol Lee Park
Hye Soo Lee
Hyun-Joong Kim
Shashi Kant Bhatia
Ranjit Gurav
Kwon-Young Choi
Yoo Kyung Lee
Yung-Hun Yang
author_sort Tae-Rim Choi
title Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic <i>Pseudomonas</i> sp. B14-6
title_short Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic <i>Pseudomonas</i> sp. B14-6
title_full Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic <i>Pseudomonas</i> sp. B14-6
title_fullStr Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic <i>Pseudomonas</i> sp. B14-6
title_full_unstemmed Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic <i>Pseudomonas</i> sp. B14-6
title_sort fructose-based production of short-chain-length and medium-chain-length polyhydroxyalkanoate copolymer by arctic <i>pseudomonas</i> sp. b14-6
publisher MDPI AG
series Polymers
issn 2073-4360
publishDate 2021-04-01
description Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium <i>Pseudomonas</i> sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes (<i>phaC</i>). <i>Pseudomonas</i> sp. B14-6 produced poly(6% 3-hydroxybutyrate-<i>co</i>-94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl-<i>co</i>-mcl PHA determined a glass transition temperature (T<sub>g</sub>) of 15.3 °C, melting temperature of 112.8 °C, crystallization temperature of 86.8 °C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 × 10<sup>4</sup>, weight average molecular weight of 9.1 × 10<sup>4</sup>, and polydispersity index value of 2.5. Overall, the novel <i>Pseudomonas</i> sp. B14-6 produced a polymer with high medium-chain-length content, low T<sub>g</sub>, and low crystallinity, indicating its potential use in medical applications.
topic <i>Pseudomonas</i> strain
polyhydroxyalkanoate
fructose syrup
fermentation
url https://www.mdpi.com/2073-4360/13/9/1398
work_keys_str_mv AT taerimchoi fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT yelimpark fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT hunsuksong fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT sunmilee fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT solleepark fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT hyesoolee fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT hyunjoongkim fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT shashikantbhatia fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT ranjitgurav fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT kwonyoungchoi fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT yookyunglee fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
AT yunghunyang fructosebasedproductionofshortchainlengthandmediumchainlengthpolyhydroxyalkanoatecopolymerbyarcticipseudomonasispb146
_version_ 1721507304668397568

Similar Items