Plasmodium falciparum gametocyte development 1 (Pfgdv1) and gametocytogenesis early gene identification and commitment to sexual development.
Malaria transmission requires the production of male and female gametocytes in the human host followed by fertilization and sporogonic development in the mosquito midgut. Although essential for the spread of malaria through the population, little is known about the initiation of gametocytogenesis in...
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2012-01-01
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doaj-c32686a4b2cb4749b8ada4beb661a10e2020-11-24T22:09:33ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742012-01-01810e100296410.1371/journal.ppat.1002964Plasmodium falciparum gametocyte development 1 (Pfgdv1) and gametocytogenesis early gene identification and commitment to sexual development.Saliha EksiBelinda J MorahanYoseph HaileTetsuya FuruyaHongying JiangOmar AliHuichun XuKirakorn KiattibutrAmreena SuriBeata CzesnyAdebowale AdeyemoTimothy G MyersJetsumon SattabongkotXin-zhuan SuKim C WilliamsonMalaria transmission requires the production of male and female gametocytes in the human host followed by fertilization and sporogonic development in the mosquito midgut. Although essential for the spread of malaria through the population, little is known about the initiation of gametocytogenesis in vitro or in vivo. Using a gametocyte-defective parasite line and genetic complementation, we show that Plasmodium falciparumgametocyte development 1 gene (Pfgdv1), encoding a peri-nuclear protein, is critical for early sexual differentiation. Transcriptional analysis of Pfgdv1 negative and positive parasite lines identified a set of gametocytogenesis early genes (Pfge) that were significantly down-regulated (>10 fold) in the absence of Pfgdv1 and expression was restored after Pfgdv1 complementation. Progressive accumulation of Pfge transcripts during successive rounds of asexual replication in synchronized cultures suggests that gametocytes are induced continuously during asexual growth. Comparison of Pfge gene transcriptional profiles in patient samples divided the genes into two groups differing in their expression in mature circulating gametocytes and providing candidates to evaluate gametocyte induction and maturation separately in vivo. The expression profile of one of the early gametocyte specific genes, Pfge1, correlated significantly with asexual parasitemia, which is consistent with the ongoing induction of gametocytogenesis during asexual growth observed in vitro and reinforces the need for sustained transmission-blocking strategies to eliminate malaria.http://europepmc.org/articles/PMC3475683?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saliha Eksi Belinda J Morahan Yoseph Haile Tetsuya Furuya Hongying Jiang Omar Ali Huichun Xu Kirakorn Kiattibutr Amreena Suri Beata Czesny Adebowale Adeyemo Timothy G Myers Jetsumon Sattabongkot Xin-zhuan Su Kim C Williamson |
spellingShingle |
Saliha Eksi Belinda J Morahan Yoseph Haile Tetsuya Furuya Hongying Jiang Omar Ali Huichun Xu Kirakorn Kiattibutr Amreena Suri Beata Czesny Adebowale Adeyemo Timothy G Myers Jetsumon Sattabongkot Xin-zhuan Su Kim C Williamson Plasmodium falciparum gametocyte development 1 (Pfgdv1) and gametocytogenesis early gene identification and commitment to sexual development. PLoS Pathogens |
author_facet |
Saliha Eksi Belinda J Morahan Yoseph Haile Tetsuya Furuya Hongying Jiang Omar Ali Huichun Xu Kirakorn Kiattibutr Amreena Suri Beata Czesny Adebowale Adeyemo Timothy G Myers Jetsumon Sattabongkot Xin-zhuan Su Kim C Williamson |
author_sort |
Saliha Eksi |
title |
Plasmodium falciparum gametocyte development 1 (Pfgdv1) and gametocytogenesis early gene identification and commitment to sexual development. |
title_short |
Plasmodium falciparum gametocyte development 1 (Pfgdv1) and gametocytogenesis early gene identification and commitment to sexual development. |
title_full |
Plasmodium falciparum gametocyte development 1 (Pfgdv1) and gametocytogenesis early gene identification and commitment to sexual development. |
title_fullStr |
Plasmodium falciparum gametocyte development 1 (Pfgdv1) and gametocytogenesis early gene identification and commitment to sexual development. |
title_full_unstemmed |
Plasmodium falciparum gametocyte development 1 (Pfgdv1) and gametocytogenesis early gene identification and commitment to sexual development. |
title_sort |
plasmodium falciparum gametocyte development 1 (pfgdv1) and gametocytogenesis early gene identification and commitment to sexual development. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2012-01-01 |
description |
Malaria transmission requires the production of male and female gametocytes in the human host followed by fertilization and sporogonic development in the mosquito midgut. Although essential for the spread of malaria through the population, little is known about the initiation of gametocytogenesis in vitro or in vivo. Using a gametocyte-defective parasite line and genetic complementation, we show that Plasmodium falciparumgametocyte development 1 gene (Pfgdv1), encoding a peri-nuclear protein, is critical for early sexual differentiation. Transcriptional analysis of Pfgdv1 negative and positive parasite lines identified a set of gametocytogenesis early genes (Pfge) that were significantly down-regulated (>10 fold) in the absence of Pfgdv1 and expression was restored after Pfgdv1 complementation. Progressive accumulation of Pfge transcripts during successive rounds of asexual replication in synchronized cultures suggests that gametocytes are induced continuously during asexual growth. Comparison of Pfge gene transcriptional profiles in patient samples divided the genes into two groups differing in their expression in mature circulating gametocytes and providing candidates to evaluate gametocyte induction and maturation separately in vivo. The expression profile of one of the early gametocyte specific genes, Pfge1, correlated significantly with asexual parasitemia, which is consistent with the ongoing induction of gametocytogenesis during asexual growth observed in vitro and reinforces the need for sustained transmission-blocking strategies to eliminate malaria. |
url |
http://europepmc.org/articles/PMC3475683?pdf=render |
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