Correlation Between Soluble Endothelial Adhesion Molecules and Nitric Oxide Metabolites in Sickle Cell Disease
Nitric Oxide (NO) and soluble adhesion molecules are promising biomarkers, which predict endothelial dysfunction in sickle cell disease (SCD). Several studies have investigated the relationship between NO (as well as its metabolites) and endothelial adhesion molecules in SCD. However, these studies...
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doaj-c32a24656c4e453ba11695aa27163a972020-11-24T23:33:11ZengMDPI AGMedical Sciences2076-32712018-12-0171110.3390/medsci7010001medsci7010001Correlation Between Soluble Endothelial Adhesion Molecules and Nitric Oxide Metabolites in Sickle Cell DiseaseCharles Antwi-Boasiako0John Ahenkorah1Eric S. Donkor2Bartholomew Dzudzor3Gifty B. Dankwah4Kate H. Otu5Robert Aryee6Charles Hayfron-Benjamin7Andrew D. Campbell8Department of Physiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra +233, GhanaDepartment of Anatomy, School of Biomedical and Allied Health Sciences, University of Ghana, Accra +233, GhanaDepartment of Medical Microbiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra +233, GhanaDepartment of Medical Biochemistry, School of Biomedical and Allied Health Sciences, University of Ghana, Accra +233, GhanaDepartment of Physiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra +233, GhanaDepartment of Nursing and Midwifery, Greenhills School of Health Sciences, Accra +233, GhanaDepartment of Physiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra +233, GhanaDepartment of Physiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra +233, GhanaCenter for Cancer and Blood Disorders Children’s National Medical Center George, Washington University School of Medicine and Health Sciences, Washington, DC 20052, USANitric Oxide (NO) and soluble adhesion molecules are promising biomarkers, which predict endothelial dysfunction in sickle cell disease (SCD). Several studies have investigated the relationship between NO (as well as its metabolites) and endothelial adhesion molecules in SCD. However, these studies were done mainly in the developed world, and it is difficult to extrapolate the findings to SCD populations in other geographical regions such as Africa due to significant disparities in the results. The aim of the current study was to determine the correlation between levels of nitric oxide metabolites (NOx) and adhesion molecules in SCD patients in a tertiary hospital in Ghana. A case control cross-sectional study involving 100 SCD (made up of HbSS and HbSC patients) and 60 healthy controls was conducted. Concentrations of NOx and soluble endothelial adhesion molecules (ICAM-1, VCAM-1 and E-selectin) were measured in all the study participants (n = 160) by the Griess reagent system and enzyme-linked immunosorbent assay (ELISA). Correlation analysis was performed to determine a possible link between the variables. Levels of soluble adhesion molecules were higher in the HbSS patients. Correlation of NOx with ICAM-1 almost approached significance (r = 0.565, <i>p</i> = 0.058) in the HbSS patients. There were no correlations between NOx and E-selectin in both HbSS and HbSC patients. There were no significant correlations between NOx and VCAM-1 in all the study participants (<i>p</i> > 0.05). Of the soluble adhesion molecules, ICAM-1 showed a significant positive correlation with VCAM-1 in the HbSC patients. There were no significant differences between the adhesion molecules and the age of participants in the various study groups. Whether or not a significant correlation exists between NOx and soluble adhesion molecules may not depend on the sickle cell genotype. The expression of adhesion molecules may not depend on age.https://www.mdpi.com/2076-3271/7/1/1sickle cell diseasesoluble adhesion moleculesnitric oxide metabolitesELISA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Charles Antwi-Boasiako John Ahenkorah Eric S. Donkor Bartholomew Dzudzor Gifty B. Dankwah Kate H. Otu Robert Aryee Charles Hayfron-Benjamin Andrew D. Campbell |
spellingShingle |
Charles Antwi-Boasiako John Ahenkorah Eric S. Donkor Bartholomew Dzudzor Gifty B. Dankwah Kate H. Otu Robert Aryee Charles Hayfron-Benjamin Andrew D. Campbell Correlation Between Soluble Endothelial Adhesion Molecules and Nitric Oxide Metabolites in Sickle Cell Disease Medical Sciences sickle cell disease soluble adhesion molecules nitric oxide metabolites ELISA |
author_facet |
Charles Antwi-Boasiako John Ahenkorah Eric S. Donkor Bartholomew Dzudzor Gifty B. Dankwah Kate H. Otu Robert Aryee Charles Hayfron-Benjamin Andrew D. Campbell |
author_sort |
Charles Antwi-Boasiako |
title |
Correlation Between Soluble Endothelial Adhesion Molecules and Nitric Oxide Metabolites in Sickle Cell Disease |
title_short |
Correlation Between Soluble Endothelial Adhesion Molecules and Nitric Oxide Metabolites in Sickle Cell Disease |
title_full |
Correlation Between Soluble Endothelial Adhesion Molecules and Nitric Oxide Metabolites in Sickle Cell Disease |
title_fullStr |
Correlation Between Soluble Endothelial Adhesion Molecules and Nitric Oxide Metabolites in Sickle Cell Disease |
title_full_unstemmed |
Correlation Between Soluble Endothelial Adhesion Molecules and Nitric Oxide Metabolites in Sickle Cell Disease |
title_sort |
correlation between soluble endothelial adhesion molecules and nitric oxide metabolites in sickle cell disease |
publisher |
MDPI AG |
series |
Medical Sciences |
issn |
2076-3271 |
publishDate |
2018-12-01 |
description |
Nitric Oxide (NO) and soluble adhesion molecules are promising biomarkers, which predict endothelial dysfunction in sickle cell disease (SCD). Several studies have investigated the relationship between NO (as well as its metabolites) and endothelial adhesion molecules in SCD. However, these studies were done mainly in the developed world, and it is difficult to extrapolate the findings to SCD populations in other geographical regions such as Africa due to significant disparities in the results. The aim of the current study was to determine the correlation between levels of nitric oxide metabolites (NOx) and adhesion molecules in SCD patients in a tertiary hospital in Ghana. A case control cross-sectional study involving 100 SCD (made up of HbSS and HbSC patients) and 60 healthy controls was conducted. Concentrations of NOx and soluble endothelial adhesion molecules (ICAM-1, VCAM-1 and E-selectin) were measured in all the study participants (n = 160) by the Griess reagent system and enzyme-linked immunosorbent assay (ELISA). Correlation analysis was performed to determine a possible link between the variables. Levels of soluble adhesion molecules were higher in the HbSS patients. Correlation of NOx with ICAM-1 almost approached significance (r = 0.565, <i>p</i> = 0.058) in the HbSS patients. There were no correlations between NOx and E-selectin in both HbSS and HbSC patients. There were no significant correlations between NOx and VCAM-1 in all the study participants (<i>p</i> > 0.05). Of the soluble adhesion molecules, ICAM-1 showed a significant positive correlation with VCAM-1 in the HbSC patients. There were no significant differences between the adhesion molecules and the age of participants in the various study groups. Whether or not a significant correlation exists between NOx and soluble adhesion molecules may not depend on the sickle cell genotype. The expression of adhesion molecules may not depend on age. |
topic |
sickle cell disease soluble adhesion molecules nitric oxide metabolites ELISA |
url |
https://www.mdpi.com/2076-3271/7/1/1 |
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