Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan
Prenatal exposure to bisphenol A (BPA) may increase the risk of abnormal birth outcomes, and DNA methylation might mediate these adverse effects. This study aimed to investigate the effects of maternal BPA exposure on maternal and fetal DNA methylation levels and explore whether epigenetic changes a...
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doaj-c32b7fdf249f4f4b98ead774adc6a5ae2021-06-30T23:29:00ZengMDPI AGInternational Journal of Environmental Research and Public Health1661-78271660-46012021-06-01186144614410.3390/ijerph18116144Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in TaiwanYu-Fang Huang0Chia-Huang Chang1Pei-Jung Chen2I-Hsuan Lin3Yen-An Tsai4Chian-Feng Chen5Yu-Chao Wang6Wei-Yun Huang7Ming-Song Tsai8Mei-Lien Chen9Department of Safety, Health and Environmental Engineering, National United University, Miaoli 360, TaiwanSchool of Public Health, Taipei Medical University, Taipei 110, TaiwanInstitute of Environmental and Occupational Health Sciences, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, TaiwanVYM Genome Research Center, National Yang Ming Chiao Tung University, Taipei 112, TaiwanInstitute of Environmental and Occupational Health Sciences, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, TaiwanVYM Genome Research Center, National Yang Ming Chiao Tung University, Taipei 112, TaiwanInstitute of Biomedical Informatics, National Yang Ming Chiao Tung University, Taipei 112, TaiwanImmuno Genomics Co., Ltd., Taipei 112, TaiwanDepartment of Obstetrics and Gynecology, Cathay General Hospital, Taipei 110, TaiwanInstitute of Environmental and Occupational Health Sciences, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, TaiwanPrenatal exposure to bisphenol A (BPA) may increase the risk of abnormal birth outcomes, and DNA methylation might mediate these adverse effects. This study aimed to investigate the effects of maternal BPA exposure on maternal and fetal DNA methylation levels and explore whether epigenetic changes are related to the associations between BPA and low birth weight. We collected urine and blood samples originating from 162 mother-infant pairs in a Taiwanese cohort study. We measured DNA methylation using the Illumina Infinium HumanMethylation 450 BeadChip in 34 maternal blood samples with high and low BPA levels based on the 75th percentile level (9.5 μg/g creatinine). Eighty-seven CpGs with the most differentially methylated probes possibly interacting with BPA exposure or birth weight were selected using two multiple regression models. Ingenuity pathway analysis (IPA) was utilized to narrow down 18 candidate CpGs related to disease categories, including developmental disorders, skeletal and muscular disorders, skeletal and muscular system development, metabolic diseases, and lipid metabolism. We then validated these genes by pyrosequencing, and 8 CpGs met the primer design score requirements in 82 cord blood samples. The associations among low birth weight, BPA exposure, and DNA methylation were analyzed. Exposure to BPA was associated with low birth weight. Analysis of the epigenome-wide findings did not show significant associations between BPA and DNA methylation in cord blood of the 8 CpGs. However, the adjusted odds ratio for the dehydrogenase/reductase member 9 (DHRS9) gene, at the 2nd CG site, in the hypermethylated group was significantly associated with low birth weight. These results support a role of BPA, and possibly DHRS9 methylation, in fetal growth. However, additional studies with larger sample sizes are warranted.https://www.mdpi.com/1660-4601/18/11/6144bisphenol ADNA methylationIllumina HumanMethylation 450 BeadChipbirth outcomes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-Fang Huang Chia-Huang Chang Pei-Jung Chen I-Hsuan Lin Yen-An Tsai Chian-Feng Chen Yu-Chao Wang Wei-Yun Huang Ming-Song Tsai Mei-Lien Chen |
spellingShingle |
Yu-Fang Huang Chia-Huang Chang Pei-Jung Chen I-Hsuan Lin Yen-An Tsai Chian-Feng Chen Yu-Chao Wang Wei-Yun Huang Ming-Song Tsai Mei-Lien Chen Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan International Journal of Environmental Research and Public Health bisphenol A DNA methylation Illumina HumanMethylation 450 BeadChip birth outcomes |
author_facet |
Yu-Fang Huang Chia-Huang Chang Pei-Jung Chen I-Hsuan Lin Yen-An Tsai Chian-Feng Chen Yu-Chao Wang Wei-Yun Huang Ming-Song Tsai Mei-Lien Chen |
author_sort |
Yu-Fang Huang |
title |
Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan |
title_short |
Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan |
title_full |
Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan |
title_fullStr |
Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan |
title_full_unstemmed |
Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan |
title_sort |
prenatal bisphenol a exposure, dna methylation, and low birth weight: a pilot study in taiwan |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1661-7827 1660-4601 |
publishDate |
2021-06-01 |
description |
Prenatal exposure to bisphenol A (BPA) may increase the risk of abnormal birth outcomes, and DNA methylation might mediate these adverse effects. This study aimed to investigate the effects of maternal BPA exposure on maternal and fetal DNA methylation levels and explore whether epigenetic changes are related to the associations between BPA and low birth weight. We collected urine and blood samples originating from 162 mother-infant pairs in a Taiwanese cohort study. We measured DNA methylation using the Illumina Infinium HumanMethylation 450 BeadChip in 34 maternal blood samples with high and low BPA levels based on the 75th percentile level (9.5 μg/g creatinine). Eighty-seven CpGs with the most differentially methylated probes possibly interacting with BPA exposure or birth weight were selected using two multiple regression models. Ingenuity pathway analysis (IPA) was utilized to narrow down 18 candidate CpGs related to disease categories, including developmental disorders, skeletal and muscular disorders, skeletal and muscular system development, metabolic diseases, and lipid metabolism. We then validated these genes by pyrosequencing, and 8 CpGs met the primer design score requirements in 82 cord blood samples. The associations among low birth weight, BPA exposure, and DNA methylation were analyzed. Exposure to BPA was associated with low birth weight. Analysis of the epigenome-wide findings did not show significant associations between BPA and DNA methylation in cord blood of the 8 CpGs. However, the adjusted odds ratio for the dehydrogenase/reductase member 9 (DHRS9) gene, at the 2nd CG site, in the hypermethylated group was significantly associated with low birth weight. These results support a role of BPA, and possibly DHRS9 methylation, in fetal growth. However, additional studies with larger sample sizes are warranted. |
topic |
bisphenol A DNA methylation Illumina HumanMethylation 450 BeadChip birth outcomes |
url |
https://www.mdpi.com/1660-4601/18/11/6144 |
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