Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs

Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs

Objectives. Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. Methods. An FFA-induced endothelial cell injury model was established to investigate...

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Main Authors: Yubin Chen, Fen Liu, Fei Han, Lizhi Lv, Can-e Tang, Fanyan Luo
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:Cardiology Research and Practice
Online Access:http://dx.doi.org/10.1155/2020/3054379
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spelling doaj-c3355441f26443d99e23dba20050751c2020-11-25T03:03:36ZengHindawi LimitedCardiology Research and Practice2090-80162090-05972020-01-01202010.1155/2020/30543793054379Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECsYubin Chen0Fen Liu1Fei Han2Lizhi Lv3Can-e Tang4Fanyan Luo5Department of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaThe Institute of Medical Science Research, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaThe Institute of Medical Science Research, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaObjectives. Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. Methods. An FFA-induced endothelial cell injury model was established to investigate the role of omentin-1 in this process. Cell proliferation was analyzed with the Cell Counting Kit assay and flow cytometry. Scratch and transwell assays were used to evaluate cell migration. Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay. Results. Omentin-1 rescued the FFA-induced impaired proliferation and migration capabilities of human umbilical vein endothelial cells (HUVECs). It decreased the number of THP-1 cells attached to HUVECs in response to injury and inhibited the FFA-induced proinflammatory state of HUVECs. Conclusion. Omentin-1 could partly ameliorate FFA-induced endothelial cell injury.http://dx.doi.org/10.1155/2020/3054379
collection DOAJ
language English
format Article
sources DOAJ
author Yubin Chen
Fen Liu
Fei Han
Lizhi Lv
Can-e Tang
Fanyan Luo
spellingShingle Yubin Chen
Fen Liu
Fei Han
Lizhi Lv
Can-e Tang
Fanyan Luo
Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
Cardiology Research and Practice
author_facet Yubin Chen
Fen Liu
Fei Han
Lizhi Lv
Can-e Tang
Fanyan Luo
author_sort Yubin Chen
title Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_short Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_full Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_fullStr Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_full_unstemmed Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_sort omentin-1 ameliorated free fatty acid-induced impairment in proliferation, migration, and inflammatory states of huvecs
publisher Hindawi Limited
series Cardiology Research and Practice
issn 2090-8016
2090-0597
publishDate 2020-01-01
description Objectives. Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. Methods. An FFA-induced endothelial cell injury model was established to investigate the role of omentin-1 in this process. Cell proliferation was analyzed with the Cell Counting Kit assay and flow cytometry. Scratch and transwell assays were used to evaluate cell migration. Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay. Results. Omentin-1 rescued the FFA-induced impaired proliferation and migration capabilities of human umbilical vein endothelial cells (HUVECs). It decreased the number of THP-1 cells attached to HUVECs in response to injury and inhibited the FFA-induced proinflammatory state of HUVECs. Conclusion. Omentin-1 could partly ameliorate FFA-induced endothelial cell injury.
url http://dx.doi.org/10.1155/2020/3054379
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