Impact of high-altitude exposure on hepatic gluconeogenesis in rats
ObjectiveTo study the effect of high altitude (HA) of 4300 m on the hepatic gluconeogenesis in rats and its underlying mechanism. MethodsThirty-six healthy adult male Sprague-Dawley (SD) rats were randomly assigned to group H1 (HA exposure for 1 day, n=6), group H3 (HA exposure for 3 days, n=6), gro...
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Editorial Department of Journal of Clinical Hepatology
2015-09-01
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doaj-c34374ef67cf423e90b2b8bb9b5993bf2020-11-25T01:33:23ZzhoEditorial Department of Journal of Clinical HepatologyLinchuang Gandanbing Zazhi1001-52561001-52562015-09-013191477148010.3969/j.issn.1001-5256.2015.09.027Impact of high-altitude exposure on hepatic gluconeogenesis in ratsNI Qian 0WAN Fengqi 1DONG Xiangyu2Department of Pediatrics, Lanzhou University Second Hospital, Lanzhou 730030, ChinaDepartment of Pediatrics, Lanzhou University Second Hospital, Lanzhou 730030, ChinaDepartment of Pediatrics, Lanzhou University Second Hospital, Lanzhou 730030, ChinaObjectiveTo study the effect of high altitude (HA) of 4300 m on the hepatic gluconeogenesis in rats and its underlying mechanism. MethodsThirty-six healthy adult male Sprague-Dawley (SD) rats were randomly assigned to group H1 (HA exposure for 1 day, n=6), group H3 (HA exposure for 3 days, n=6), group H7 (HA exposure for 7 days, n=6), group H15 (HA exposure for 15 days, n=6), group H30 (HA exposure for 30 days, n=6), and group C (no HA exposure, n=6). After the treatment, the mRNA and protein levels of glucose-6-phosphatase (G6Pase), and forkhead box transcription factor O1 (FoxO1) in the hepatic tissues were determined by RT-PCR and Western blot, respectively. The content of hepatic glycogen was determined by spectrophotometry, and the blood glucose level was measured using an automatic biochemical analyzer. The one-way analysis of variance (ANOVA) was used to analyze the differences between groups, and the Tukey test was further used to compare the differences between two groups. ResultsCompared with those in group C, the levels of G6Pase and glycogen in the hepatic tissues of rats increased significantly in groups H1, H3, and H7 (P<0.05), and the expression level of FoxO1 decreased significantly in groups H3, H7, H15, and H30 (P<0.01). No significant differences in the concentration of blood glucose were observed between the HA-treated groups. ConclusionIncreased hepatic gluconeogenesis and glycogen synthesis in the early phase of HA exposure may be one of the important mechanisms of HA acclimatization. FoxO1 and AMPK are involved in the regulation of hepatic gluconeogenesis. The increased content of hepatic glycogen is associated with the decreased activity of AMPK.http://www.lcgdbzz.org/qk_content.asp?id=6762 |
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DOAJ |
language |
zho |
format |
Article |
sources |
DOAJ |
author |
NI Qian WAN Fengqi DONG Xiangyu |
spellingShingle |
NI Qian WAN Fengqi DONG Xiangyu Impact of high-altitude exposure on hepatic gluconeogenesis in rats Linchuang Gandanbing Zazhi |
author_facet |
NI Qian WAN Fengqi DONG Xiangyu |
author_sort |
NI Qian |
title |
Impact of high-altitude exposure on hepatic gluconeogenesis in rats |
title_short |
Impact of high-altitude exposure on hepatic gluconeogenesis in rats |
title_full |
Impact of high-altitude exposure on hepatic gluconeogenesis in rats |
title_fullStr |
Impact of high-altitude exposure on hepatic gluconeogenesis in rats |
title_full_unstemmed |
Impact of high-altitude exposure on hepatic gluconeogenesis in rats |
title_sort |
impact of high-altitude exposure on hepatic gluconeogenesis in rats |
publisher |
Editorial Department of Journal of Clinical Hepatology |
series |
Linchuang Gandanbing Zazhi |
issn |
1001-5256 1001-5256 |
publishDate |
2015-09-01 |
description |
ObjectiveTo study the effect of high altitude (HA) of 4300 m on the hepatic gluconeogenesis in rats and its underlying mechanism. MethodsThirty-six healthy adult male Sprague-Dawley (SD) rats were randomly assigned to group H1 (HA exposure for 1 day, n=6), group H3 (HA exposure for 3 days, n=6), group H7 (HA exposure for 7 days, n=6), group H15 (HA exposure for 15 days, n=6), group H30 (HA exposure for 30 days, n=6), and group C (no HA exposure, n=6). After the treatment, the mRNA and protein levels of glucose-6-phosphatase (G6Pase), and forkhead box transcription factor O1 (FoxO1) in the hepatic tissues were determined by RT-PCR and Western blot, respectively. The content of hepatic glycogen was determined by spectrophotometry, and the blood glucose level was measured using an automatic biochemical analyzer. The one-way analysis of variance (ANOVA) was used to analyze the differences between groups, and the Tukey test was further used to compare the differences between two groups. ResultsCompared with those in group C, the levels of G6Pase and glycogen in the hepatic tissues of rats increased significantly in groups H1, H3, and H7 (P<0.05), and the expression level of FoxO1 decreased significantly in groups H3, H7, H15, and H30 (P<0.01). No significant differences in the concentration of blood glucose were observed between the HA-treated groups. ConclusionIncreased hepatic gluconeogenesis and glycogen synthesis in the early phase of HA exposure may be one of the important mechanisms of HA acclimatization. FoxO1 and AMPK are involved in the regulation of hepatic gluconeogenesis. The increased content of hepatic glycogen is associated with the decreased activity of AMPK. |
url |
http://www.lcgdbzz.org/qk_content.asp?id=6762 |
work_keys_str_mv |
AT niqian impactofhighaltitudeexposureonhepaticgluconeogenesisinrats AT wanfengqi impactofhighaltitudeexposureonhepaticgluconeogenesisinrats AT dongxiangyu impactofhighaltitudeexposureonhepaticgluconeogenesisinrats |
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