A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy

A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy

Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potent...

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Main Authors: Kanako Miyano, Katsuya Ohbuchi, Yuka Sudo, Kouichiro Minami, Toru Yokoyama, Masahiro Yamamoto, Miaki Uzu, Miki Nonaka, Seiji Shiraishi, Hiroaki Murata, Yoshikazu Higami, Yasuhito Uezono
Format: Article
Language:English
Published: Elsevier 2020-08-01
Series:Journal of Pharmacological Sciences
Subjects:
TRPV1
Cellular dielectric spectroscopy
CellKey™ system
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861320300451
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spelling doaj-c353329a3d5446a2926a09e994c6518d2020-11-25T02:24:57ZengElsevierJournal of Pharmacological Sciences1347-86132020-08-011434320324A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopyKanako Miyano0Katsuya Ohbuchi1Yuka Sudo2Kouichiro Minami3Toru Yokoyama4Masahiro Yamamoto5Miaki Uzu6Miki Nonaka7Seiji Shiraishi8Hiroaki Murata9Yoshikazu Higami10Yasuhito Uezono11Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, JapanTsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, JapanTsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, JapanDepartment of Anesthesiology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, JapanLaboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan; Division of Supportive Care Research, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Tokyo 104-0045, Japan; Innovation Center for Supportive, Palliative and Psychosocial Care, National Cancer Center Hospital, Tokyo 104-0045, Japan; Department of Comprehensive Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan; Corresponding author. Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan. Fax: +81 3 3542 0688.Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC50) and efficacy (Emax) of these agonists with CellKey™ were almost identical to those made using the traditional Ca2+ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.http://www.sciencedirect.com/science/article/pii/S1347861320300451TRPV1Cellular dielectric spectroscopyCellKey™ system
collection DOAJ
language English
format Article
sources DOAJ
author Kanako Miyano
Katsuya Ohbuchi
Yuka Sudo
Kouichiro Minami
Toru Yokoyama
Masahiro Yamamoto
Miaki Uzu
Miki Nonaka
Seiji Shiraishi
Hiroaki Murata
Yoshikazu Higami
Yasuhito Uezono
spellingShingle Kanako Miyano
Katsuya Ohbuchi
Yuka Sudo
Kouichiro Minami
Toru Yokoyama
Masahiro Yamamoto
Miaki Uzu
Miki Nonaka
Seiji Shiraishi
Hiroaki Murata
Yoshikazu Higami
Yasuhito Uezono
A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
Journal of Pharmacological Sciences
TRPV1
Cellular dielectric spectroscopy
CellKey™ system
author_facet Kanako Miyano
Katsuya Ohbuchi
Yuka Sudo
Kouichiro Minami
Toru Yokoyama
Masahiro Yamamoto
Miaki Uzu
Miki Nonaka
Seiji Shiraishi
Hiroaki Murata
Yoshikazu Higami
Yasuhito Uezono
author_sort Kanako Miyano
title A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
title_short A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
title_full A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
title_fullStr A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
title_full_unstemmed A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
title_sort novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2020-08-01
description Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC50) and efficacy (Emax) of these agonists with CellKey™ were almost identical to those made using the traditional Ca2+ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.
topic TRPV1
Cellular dielectric spectroscopy
CellKey™ system
url http://www.sciencedirect.com/science/article/pii/S1347861320300451
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