A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potent...
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doaj-c353329a3d5446a2926a09e994c6518d2020-11-25T02:24:57ZengElsevierJournal of Pharmacological Sciences1347-86132020-08-011434320324A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopyKanako Miyano0Katsuya Ohbuchi1Yuka Sudo2Kouichiro Minami3Toru Yokoyama4Masahiro Yamamoto5Miaki Uzu6Miki Nonaka7Seiji Shiraishi8Hiroaki Murata9Yoshikazu Higami10Yasuhito Uezono11Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, JapanTsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, JapanTsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, JapanDepartment of Anesthesiology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, JapanLaboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, JapanDivision of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan; Division of Supportive Care Research, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Tokyo 104-0045, Japan; Innovation Center for Supportive, Palliative and Psychosocial Care, National Cancer Center Hospital, Tokyo 104-0045, Japan; Department of Comprehensive Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan; Corresponding author. Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan. Fax: +81 3 3542 0688.Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC50) and efficacy (Emax) of these agonists with CellKey™ were almost identical to those made using the traditional Ca2+ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.http://www.sciencedirect.com/science/article/pii/S1347861320300451TRPV1Cellular dielectric spectroscopyCellKey™ system |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kanako Miyano Katsuya Ohbuchi Yuka Sudo Kouichiro Minami Toru Yokoyama Masahiro Yamamoto Miaki Uzu Miki Nonaka Seiji Shiraishi Hiroaki Murata Yoshikazu Higami Yasuhito Uezono |
spellingShingle |
Kanako Miyano Katsuya Ohbuchi Yuka Sudo Kouichiro Minami Toru Yokoyama Masahiro Yamamoto Miaki Uzu Miki Nonaka Seiji Shiraishi Hiroaki Murata Yoshikazu Higami Yasuhito Uezono A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy Journal of Pharmacological Sciences TRPV1 Cellular dielectric spectroscopy CellKey™ system |
author_facet |
Kanako Miyano Katsuya Ohbuchi Yuka Sudo Kouichiro Minami Toru Yokoyama Masahiro Yamamoto Miaki Uzu Miki Nonaka Seiji Shiraishi Hiroaki Murata Yoshikazu Higami Yasuhito Uezono |
author_sort |
Kanako Miyano |
title |
A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy |
title_short |
A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy |
title_full |
A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy |
title_fullStr |
A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy |
title_full_unstemmed |
A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy |
title_sort |
novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy |
publisher |
Elsevier |
series |
Journal of Pharmacological Sciences |
issn |
1347-8613 |
publishDate |
2020-08-01 |
description |
Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC50) and efficacy (Emax) of these agonists with CellKey™ were almost identical to those made using the traditional Ca2+ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities. |
topic |
TRPV1 Cellular dielectric spectroscopy CellKey™ system |
url |
http://www.sciencedirect.com/science/article/pii/S1347861320300451 |
work_keys_str_mv |
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