Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomerase
Abstract Background Giardia duodenalis causes giardiasis, with diarrhea as the primary symptom. The trophozoite proliferation of this zoonotic parasite is mainly affected by telomerase, although the mechanism of telomerase regulation has not been thoroughly analyzed. Methods This study was performed...
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doaj-c37cf7e720a14216bfe39e6441ae4e2e2020-11-29T12:05:48ZengBMCParasites & Vectors1756-33052019-11-0112111110.1186/s13071-019-3821-0Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomeraseJing-Tong Zheng0Nan Zhang1Yan-Hui Yu2Peng-Tao Gong3Xian-He Li4Na Wu5Can Wang6Xiao-Cen Wang7Xin Li8Jian-Hua Li9Xi-Chen Zhang10Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityClinical Laboratory of Second Hospital, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin UniversityAbstract Background Giardia duodenalis causes giardiasis, with diarrhea as the primary symptom. The trophozoite proliferation of this zoonotic parasite is mainly affected by telomerase, although the mechanism of telomerase regulation has not been thoroughly analyzed. Methods This study was performed to identify the telomerase RNA-binding domain (TRBD)-interacting protein in G. duodenalis and its regulation of telomerase. Interaction between TRBD and interacting proteins was verified via pulldown assays and co-immunoprecipitation (co-IP) techniques, and the subcellular localization of the protein interactions was determined in vivo via split SNAP-tag labeling. The hammerhead ribozyme was designed to deplete the mRNA of TRBD-interacting proteins. Results Using TRBD as bait, we identified zinc-finger domain (ZFD)-containing proteins and verified it via pulldown and co-IP experiments. Protein-protein interaction occurred in the nuclei of 293T cells and both nuclei of G. duodenalis. The hammerhead ribozyme depleted ZFD mRNA levels, which reduced the reproduction rate of G. duodenalis, telomerase activity and telomere length. Conclusions Our findings suggest that ZFD may regulate telomere function in G. duodenalis nuclei.https://doi.org/10.1186/s13071-019-3821-0Giardia duodenalisTelomeraseProtein–protein interaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jing-Tong Zheng Nan Zhang Yan-Hui Yu Peng-Tao Gong Xian-He Li Na Wu Can Wang Xiao-Cen Wang Xin Li Jian-Hua Li Xi-Chen Zhang |
spellingShingle |
Jing-Tong Zheng Nan Zhang Yan-Hui Yu Peng-Tao Gong Xian-He Li Na Wu Can Wang Xiao-Cen Wang Xin Li Jian-Hua Li Xi-Chen Zhang Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomerase Parasites & Vectors Giardia duodenalis Telomerase Protein–protein interaction |
author_facet |
Jing-Tong Zheng Nan Zhang Yan-Hui Yu Peng-Tao Gong Xian-He Li Na Wu Can Wang Xiao-Cen Wang Xin Li Jian-Hua Li Xi-Chen Zhang |
author_sort |
Jing-Tong Zheng |
title |
Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomerase |
title_short |
Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomerase |
title_full |
Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomerase |
title_fullStr |
Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomerase |
title_full_unstemmed |
Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomerase |
title_sort |
identification of a trbd zinc finger-interacting protein in giardia duodenalis and its regulation of telomerase |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2019-11-01 |
description |
Abstract Background Giardia duodenalis causes giardiasis, with diarrhea as the primary symptom. The trophozoite proliferation of this zoonotic parasite is mainly affected by telomerase, although the mechanism of telomerase regulation has not been thoroughly analyzed. Methods This study was performed to identify the telomerase RNA-binding domain (TRBD)-interacting protein in G. duodenalis and its regulation of telomerase. Interaction between TRBD and interacting proteins was verified via pulldown assays and co-immunoprecipitation (co-IP) techniques, and the subcellular localization of the protein interactions was determined in vivo via split SNAP-tag labeling. The hammerhead ribozyme was designed to deplete the mRNA of TRBD-interacting proteins. Results Using TRBD as bait, we identified zinc-finger domain (ZFD)-containing proteins and verified it via pulldown and co-IP experiments. Protein-protein interaction occurred in the nuclei of 293T cells and both nuclei of G. duodenalis. The hammerhead ribozyme depleted ZFD mRNA levels, which reduced the reproduction rate of G. duodenalis, telomerase activity and telomere length. Conclusions Our findings suggest that ZFD may regulate telomere function in G. duodenalis nuclei. |
topic |
Giardia duodenalis Telomerase Protein–protein interaction |
url |
https://doi.org/10.1186/s13071-019-3821-0 |
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