Bioconjugated Pluronic Triblock-Copolymer Micelle-Encapsulated Quantum Dots for Targeted Imaging of Cancer: In Vitro and In Vivo Studies

• Main Author: Liwei Liu, Ken-Tye Yong, Indrajit Roy, Wing-Cheung Law, Ling Ye, Jianwei Liu, Jing Liu, Rajiv Kumar, Xihe Zhang, Paras N. Prasad
• Format: Article
• Language: English
• Published: Ivyspring International Publisher 2012-01-01
• Series: Theranostics
• Online Access: http://www.thno.org/v02p0705.htm

<p>Early in this study, CdTe/ZnS core/shell quantum dots (QDs) were encapsulated in carboxylated Pluronic F127 triblock polymeric micelle, to preserve the optical and colloidal stability of QDs in biological fluids. Folic acid (FA) was then conjugated to the surface of QDs for the targeted delivery of the QD formulation to the tumor site, by exploiting the overexpressed FA receptors (FARs) on the tumor cells. Cytotoxicity study demonstrated that the QD formulation has negligible <i>in vitro</i> toxicity. The <i>in vitro</i> study showed that the bioconjugated micelle-encapsulated QDs, but not the unconjugated QDs, were able to efficiently label Panc-1 cancer cells. <i>In vivo</i> imaging study showed that bioconjugated QDs were able to target tumor site after intravenous injection of the formulation in tumor-bearing mice.</p>