Nitrate-Induced Inhibition of Root Nodule Formation and Nitrogenase Activity in the Peanut (Arachis hypogaea L.)
The effect of nitrate in the culture solution (0.7, 3.5, 7.0, and 14.0 mM N) on the growth, root nodule formation and nitrogenase activity of the peanut (cv. chico) inoculated with Bradyrhizobium sp. was examined at early developmental stages. The amount of nitrogen derived from fixation and its per...
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1999-01-01
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Online Access: | http://dx.doi.org/10.1626/pps.2.81 |
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doaj-c399ece18665414ba61ddb77c96e9f4d2020-11-24T22:50:03ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10081999-01-0122818610.1626/pps.2.8111644354Nitrate-Induced Inhibition of Root Nodule Formation and Nitrogenase Activity in the Peanut (Arachis hypogaea L.)Hiroyuki Daimon0Kenji Hori1Asuka Shimizu2Masao Nakagawa3Osaka Prefecture UniversityOsaka Prefecture UniversityOsaka Prefecture UniversityOsaka Prefecture UniversityThe effect of nitrate in the culture solution (0.7, 3.5, 7.0, and 14.0 mM N) on the growth, root nodule formation and nitrogenase activity of the peanut (cv. chico) inoculated with Bradyrhizobium sp. was examined at early developmental stages. The amount of nitrogen derived from fixation and its percentage to total nitrogen in the nitrate-fed plant were evaluated using 15N labeled nitrate. The dry weight and total nitrogen content of the plant at 40 days after transplanting (day 40) varied with the concentration of nitrate in the culture solution. Both of them were higher in the plants grown in the presence of nitrate at the concentrations above 0.7 mM. On the other hand, the number and fresh weight of nodules, and acetylene reduction activity per plant were the lowest in the presence of nitrate at 14.0 mM, relatively high at 0.7 mM, and the highest at 3.5 mM. The proportion of N derived from atmosphere to total nitrogen in the plants examined at days 18 and 33 was 23–31% in the presence of nitrate at 3.5 and 14.0 mM. However, the proportion examined at day 48 was as high as 57–58% at 3.5 mM, and it was only 22–24% at 14.0 mM.http://dx.doi.org/10.1626/pps.2.81Acetylene reduction activityArachis hypogaea LBradyrhizobiumNitrate15NNitrogen fixationPeanutRoot nodule |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hiroyuki Daimon Kenji Hori Asuka Shimizu Masao Nakagawa |
spellingShingle |
Hiroyuki Daimon Kenji Hori Asuka Shimizu Masao Nakagawa Nitrate-Induced Inhibition of Root Nodule Formation and Nitrogenase Activity in the Peanut (Arachis hypogaea L.) Plant Production Science Acetylene reduction activity Arachis hypogaea L Bradyrhizobium Nitrate 15N Nitrogen fixation Peanut Root nodule |
author_facet |
Hiroyuki Daimon Kenji Hori Asuka Shimizu Masao Nakagawa |
author_sort |
Hiroyuki Daimon |
title |
Nitrate-Induced Inhibition of Root Nodule Formation and Nitrogenase Activity in the Peanut (Arachis hypogaea L.) |
title_short |
Nitrate-Induced Inhibition of Root Nodule Formation and Nitrogenase Activity in the Peanut (Arachis hypogaea L.) |
title_full |
Nitrate-Induced Inhibition of Root Nodule Formation and Nitrogenase Activity in the Peanut (Arachis hypogaea L.) |
title_fullStr |
Nitrate-Induced Inhibition of Root Nodule Formation and Nitrogenase Activity in the Peanut (Arachis hypogaea L.) |
title_full_unstemmed |
Nitrate-Induced Inhibition of Root Nodule Formation and Nitrogenase Activity in the Peanut (Arachis hypogaea L.) |
title_sort |
nitrate-induced inhibition of root nodule formation and nitrogenase activity in the peanut (arachis hypogaea l.) |
publisher |
Taylor & Francis Group |
series |
Plant Production Science |
issn |
1343-943X 1349-1008 |
publishDate |
1999-01-01 |
description |
The effect of nitrate in the culture solution (0.7, 3.5, 7.0, and 14.0 mM N) on the growth, root nodule formation and nitrogenase activity of the peanut (cv. chico) inoculated with Bradyrhizobium sp. was examined at early developmental stages. The amount of nitrogen derived from fixation and its percentage to total nitrogen in the nitrate-fed plant were evaluated using 15N labeled nitrate. The dry weight and total nitrogen content of the plant at 40 days after transplanting (day 40) varied with the concentration of nitrate in the culture solution. Both of them were higher in the plants grown in the presence of nitrate at the concentrations above 0.7 mM. On the other hand, the number and fresh weight of nodules, and acetylene reduction activity per plant were the lowest in the presence of nitrate at 14.0 mM, relatively high at 0.7 mM, and the highest at 3.5 mM. The proportion of N derived from atmosphere to total nitrogen in the plants examined at days 18 and 33 was 23–31% in the presence of nitrate at 3.5 and 14.0 mM. However, the proportion examined at day 48 was as high as 57–58% at 3.5 mM, and it was only 22–24% at 14.0 mM. |
topic |
Acetylene reduction activity Arachis hypogaea L Bradyrhizobium Nitrate 15N Nitrogen fixation Peanut Root nodule |
url |
http://dx.doi.org/10.1626/pps.2.81 |
work_keys_str_mv |
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