Single‐cell analyses reveal suppressive tumor microenvironment of human colorectal cancer
Abstract Profiling heterologous cell types within tumors is essential to decipher tumor microenvironment that shapes tumor progress and determines the outcome of therapeutic response. Here, we comprehensively characterized transcriptomes of 34,037 single cells obtained from 12 treatment‐naïve patien...
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doaj-c3b029027e0744c0a6aef528f30a93812021-07-13T11:05:29ZengWileyClinical and Translational Medicine2001-13262021-06-01116n/an/a10.1002/ctm2.422Single‐cell analyses reveal suppressive tumor microenvironment of human colorectal cancerYan Mei0Weiwei Xiao1Hao Hu2Guanming Lu3Lingdan Chen4Zhun Sun5Mengdie Lü6Wenhui Ma7Ting Jiang8YuanHong Gao9LiRen Li10Gong Chen11Zifeng Wang12Hanjie Li13Duojiao Wu14Pinghong Zhou15Qibin Leng16Guangshuai Jia17Affiliated Cancer Hospital and Institute of Guangzhou Medical University State Key Laboratory of Respiratory Disease Guangzhou ChinaDepartment of Radiation Oncology Sun Yat‐sen University Cancer Center Guangzhou ChinaEndoscopy Center Zhongshan Hospital, Fudan University Shanghai ChinaDepartment of Breast and Thyroid Surgery Affiliated hospital of Youjiang Medical University for Nationalities Baise Guangxi ChinaAffiliated Cancer Hospital and Institute of Guangzhou Medical University State Key Laboratory of Respiratory Disease Guangzhou ChinaAffiliated Cancer Hospital and Institute of Guangzhou Medical University State Key Laboratory of Respiratory Disease Guangzhou ChinaAffiliated Cancer Hospital and Institute of Guangzhou Medical University State Key Laboratory of Respiratory Disease Guangzhou ChinaDepartment of General Surgery, Nanfang Hospital Southern Medical University Guangzhou ChinaDepartment of Radiation Oncology Sun Yat‐sen University Cancer Center Guangzhou ChinaDepartment of Radiation Oncology Sun Yat‐sen University Cancer Center Guangzhou ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine Sun Yat‐Sen University Cancer Center Guangzhou ChinaCenter for Synthetic Immunology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen ChinaInstitute of Clinical Science, Zhongshan Hospital, Shanghai Institute of Clinical Bioinformatics Fudan University Shanghai ChinaEndoscopy Center Zhongshan Hospital, Fudan University Shanghai ChinaAffiliated Cancer Hospital and Institute of Guangzhou Medical University State Key Laboratory of Respiratory Disease Guangzhou ChinaAffiliated Cancer Hospital and Institute of Guangzhou Medical University State Key Laboratory of Respiratory Disease Guangzhou ChinaAbstract Profiling heterologous cell types within tumors is essential to decipher tumor microenvironment that shapes tumor progress and determines the outcome of therapeutic response. Here, we comprehensively characterized transcriptomes of 34,037 single cells obtained from 12 treatment‐naïve patients with colorectal cancer. Our comprehensive evaluation revealed attenuated B‐cell antigen presentation, distinct regulatory T‐cell clusters with different origin and novel polyfunctional tumor associated macrophages associated with CRC. Moreover, we identified expanded XCL1+ T‐cell clusters associated with tumor mutational burden high status. We further explored the underlying molecular mechanisms by profiling epigenetic landscape and inferring transcription factor motifs using single‐cell ATAC‐seq. Our dataset and analysis approaches herein provide a rich resource for further study of the impact of immune cells and translational research for human colorectal cancer.https://doi.org/10.1002/ctm2.422human colorectal cancerscATAC‐seqscRNA‐seqtumor immune microenvironment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan Mei Weiwei Xiao Hao Hu Guanming Lu Lingdan Chen Zhun Sun Mengdie Lü Wenhui Ma Ting Jiang YuanHong Gao LiRen Li Gong Chen Zifeng Wang Hanjie Li Duojiao Wu Pinghong Zhou Qibin Leng Guangshuai Jia |
spellingShingle |
Yan Mei Weiwei Xiao Hao Hu Guanming Lu Lingdan Chen Zhun Sun Mengdie Lü Wenhui Ma Ting Jiang YuanHong Gao LiRen Li Gong Chen Zifeng Wang Hanjie Li Duojiao Wu Pinghong Zhou Qibin Leng Guangshuai Jia Single‐cell analyses reveal suppressive tumor microenvironment of human colorectal cancer Clinical and Translational Medicine human colorectal cancer scATAC‐seq scRNA‐seq tumor immune microenvironment |
author_facet |
Yan Mei Weiwei Xiao Hao Hu Guanming Lu Lingdan Chen Zhun Sun Mengdie Lü Wenhui Ma Ting Jiang YuanHong Gao LiRen Li Gong Chen Zifeng Wang Hanjie Li Duojiao Wu Pinghong Zhou Qibin Leng Guangshuai Jia |
author_sort |
Yan Mei |
title |
Single‐cell analyses reveal suppressive tumor microenvironment of human colorectal cancer |
title_short |
Single‐cell analyses reveal suppressive tumor microenvironment of human colorectal cancer |
title_full |
Single‐cell analyses reveal suppressive tumor microenvironment of human colorectal cancer |
title_fullStr |
Single‐cell analyses reveal suppressive tumor microenvironment of human colorectal cancer |
title_full_unstemmed |
Single‐cell analyses reveal suppressive tumor microenvironment of human colorectal cancer |
title_sort |
single‐cell analyses reveal suppressive tumor microenvironment of human colorectal cancer |
publisher |
Wiley |
series |
Clinical and Translational Medicine |
issn |
2001-1326 |
publishDate |
2021-06-01 |
description |
Abstract Profiling heterologous cell types within tumors is essential to decipher tumor microenvironment that shapes tumor progress and determines the outcome of therapeutic response. Here, we comprehensively characterized transcriptomes of 34,037 single cells obtained from 12 treatment‐naïve patients with colorectal cancer. Our comprehensive evaluation revealed attenuated B‐cell antigen presentation, distinct regulatory T‐cell clusters with different origin and novel polyfunctional tumor associated macrophages associated with CRC. Moreover, we identified expanded XCL1+ T‐cell clusters associated with tumor mutational burden high status. We further explored the underlying molecular mechanisms by profiling epigenetic landscape and inferring transcription factor motifs using single‐cell ATAC‐seq. Our dataset and analysis approaches herein provide a rich resource for further study of the impact of immune cells and translational research for human colorectal cancer. |
topic |
human colorectal cancer scATAC‐seq scRNA‐seq tumor immune microenvironment |
url |
https://doi.org/10.1002/ctm2.422 |
work_keys_str_mv |
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