Inhibition of Aflatoxin Production by Paraquat and External Superoxide Dismutase in <i>Aspergillus flavus</i>

• Main Authors: Tomohiro Furukawa, Shohei Sakuda
• Format: Article
• Language: English
• Published: MDPI AG 2019-02-01
• Series: Toxins
• Subjects: aflatoxin production; <i>Aspergillus flavus</i>; superoxide; paraquat; superoxide dismutase
• Online Access: https://www.mdpi.com/2072-6651/11/2/107

Aflatoxin contamination of crops is a worldwide problem, and elucidation of the regulatory mechanism of aflatoxin production, for example relative to the oxidative&#8315;antioxidative system, is needed. Studies have shown that oxidative stress induced by reactive oxygen species promotes aflatoxin production. However, superoxide has been suggested to have the opposite effect. Here, we investigated the effects of the superoxide generator, paraquat, and externally added superoxide dismutase (SOD) on aflatoxin production in <i>Aspergillus flavus</i>. Paraquat with an IC₅₀ value of 54.9 &#181;M inhibited aflatoxin production without affecting fungal growth. It increased cytosolic and mitochondrial superoxide levels and downregulated the transcription of aflatoxin biosynthetic cluster genes, including <i>aflR</i>, a key regulatory protein. The addition of bovine Cu/ZnSOD to the culture medium suppressed the paraquat-induced increase in superoxide levels, but it did not fully restore paraquat-inhibited aflatoxin production because bovine Cu/ZnSOD with an IC₅₀ value of 17.9 &#181;g/mL itself inhibited aflatoxin production. Externally added bovine Cu/ZnSOD increased the SOD activity in fungal cell extracts and upregulated the transcription of genes encoding Cu/ZnSOD and alcohol dehydrogenase. These results suggest that intracellular accumulation of superoxide impairs aflatoxin production by downregulating <i>aflR</i> expression, and that externally added Cu/ZnSOD also suppresses aflatoxin production by a mechanism other than canonical superoxide elimination activity.