Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota
Eight fermentative bacterial strains were isolated from mixed enrichment cultures of a composite soil sample collected at 1.34 km depth from the former Homestake gold mine in Lead, SD, USA. Phylogenetic analysis of their 16S rRNA gene sequences revealed that these isolates were affiliated with the...
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doaj-c3ce7a2f00f94c23bc9b67719d744e072020-11-24T22:36:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2013-02-01410.3389/fmicb.2013.0001840280Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South DakotaGurdeep eRastogi0Raghunandan eGurram1Aditya eBhalla2Ramon eGonzalez3Kenneth eBischoff4Stephen eHughes5Sudhir eKumar6Rajesh Kumar Sani7South Dakota School of Mines and TechnologySouth Dakota School of Mines and TechnologySouth Dakota School of Mines and TechnologyDepartments of Chemical & Biomolecular Engineering and Bioengineering, Rice UniversityNational Center for Agricultural Utilization Research, U.S. Department of AgricultureNational Center for Agricultural Utilization Research, U.S. Department of AgricultureSouth Dakota School of Mines and TechnologySouth Dakota School of Mines and TechnologyEight fermentative bacterial strains were isolated from mixed enrichment cultures of a composite soil sample collected at 1.34 km depth from the former Homestake gold mine in Lead, SD, USA. Phylogenetic analysis of their 16S rRNA gene sequences revealed that these isolates were affiliated with the phylum Firmicutes belonging to genera Bacillus and Clostridium. Batch fermentation studies demonstrated that isolates had the ability to ferment glucose, xylose, or glycerol to industrially valuable products such as ethanol and 1,3-propanediol (PDO).Batch fermentation studies on selected seven bacterial strains demonstrated that these have the ability to ferment glucose, xylose, or glycerol to industrially valuable products such as ethanol and 1,3-propanediol (PDO). Ethanol was detected as the major fermentation end product in glucose-fermenting cultures at pH 10 with yields of 0.206 - 0.304 g of ethanol/g of glucose. While a xylose-fermenting strain yielded 0.189 g of ethanol/g of xylose and 0.585 g of acetic acid/g of xylose at the end of fermentation. At pH 7, glycerol-fermenting isolates produced PDO (0.323 - 0.458 g of PDO/g of glycerol) and ethanol (0.284 - 0.350 g of ethanol/g of glycerol) as major end products while acetic acid and succinic acid were identified as minor by-products in fermentation broths. These results suggest that the deep biosphere of the former Homestake gold mine harbors bacterial strains which could be used in bio-based production of ethanol and PDO.http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00018/fullFermentationbioenergybioethanolGold mineBiofules1 3 propandiol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gurdeep eRastogi Raghunandan eGurram Aditya eBhalla Ramon eGonzalez Kenneth eBischoff Stephen eHughes Sudhir eKumar Rajesh Kumar Sani |
spellingShingle |
Gurdeep eRastogi Raghunandan eGurram Aditya eBhalla Ramon eGonzalez Kenneth eBischoff Stephen eHughes Sudhir eKumar Rajesh Kumar Sani Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota Frontiers in Microbiology Fermentation bioenergy bioethanol Gold mine Biofules 1 3 propandiol |
author_facet |
Gurdeep eRastogi Raghunandan eGurram Aditya eBhalla Ramon eGonzalez Kenneth eBischoff Stephen eHughes Sudhir eKumar Rajesh Kumar Sani |
author_sort |
Gurdeep eRastogi |
title |
Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota |
title_short |
Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota |
title_full |
Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota |
title_fullStr |
Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota |
title_full_unstemmed |
Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota |
title_sort |
presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former homestake gold mine, south dakota |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2013-02-01 |
description |
Eight fermentative bacterial strains were isolated from mixed enrichment cultures of a composite soil sample collected at 1.34 km depth from the former Homestake gold mine in Lead, SD, USA. Phylogenetic analysis of their 16S rRNA gene sequences revealed that these isolates were affiliated with the phylum Firmicutes belonging to genera Bacillus and Clostridium. Batch fermentation studies demonstrated that isolates had the ability to ferment glucose, xylose, or glycerol to industrially valuable products such as ethanol and 1,3-propanediol (PDO).Batch fermentation studies on selected seven bacterial strains demonstrated that these have the ability to ferment glucose, xylose, or glycerol to industrially valuable products such as ethanol and 1,3-propanediol (PDO). Ethanol was detected as the major fermentation end product in glucose-fermenting cultures at pH 10 with yields of 0.206 - 0.304 g of ethanol/g of glucose. While a xylose-fermenting strain yielded 0.189 g of ethanol/g of xylose and 0.585 g of acetic acid/g of xylose at the end of fermentation. At pH 7, glycerol-fermenting isolates produced PDO (0.323 - 0.458 g of PDO/g of glycerol) and ethanol (0.284 - 0.350 g of ethanol/g of glycerol) as major end products while acetic acid and succinic acid were identified as minor by-products in fermentation broths. These results suggest that the deep biosphere of the former Homestake gold mine harbors bacterial strains which could be used in bio-based production of ethanol and PDO. |
topic |
Fermentation bioenergy bioethanol Gold mine Biofules 1 3 propandiol |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00018/full |
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