Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells
AIM: To explore the possibility of human umbilical cord mesenchymal stem cells (hUCMSCs), human umbilical vein endothelial cells (hUVECs), human dental pulp stem cells (hDPSCs) and human periodontal ligament stem cells (hPDLSCs) serving as feeder cells in co-culture systems for the cultivation of li...
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doaj-c3dad94891e14ba7970b647b913356c72020-11-25T00:33:51ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982017-07-011071021102710.18240/ijo.2017.07.01Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cellsHui-Xian Wang0Xiao-Wei Gao1Bing Ren2Yan Cai3Wen-Jing Li4Yu-Li Yang5Yi-Jian Li6Medical College of Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, China; Ophthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur Autonomous Region, ChinaOphthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur Autonomous Region, ChinaOphthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur Autonomous Region, ChinaOphthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur Autonomous Region, ChinaOphthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur Autonomous Region, ChinaSouthwest Hospital, Third Military Medical University, Chongqing 400038, ChinaSouthwest Hospital, Third Military Medical University, Chongqing 400038, ChinaAIM: To explore the possibility of human umbilical cord mesenchymal stem cells (hUCMSCs), human umbilical vein endothelial cells (hUVECs), human dental pulp stem cells (hDPSCs) and human periodontal ligament stem cells (hPDLSCs) serving as feeder cells in co-culture systems for the cultivation of limbal stem cells. METHODS: Different feeder layers were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F12 and were treated with mitomycin C. Rabbits limbal stem cells (LSCs) were co-cultured on hUCMSCs, hUVECs, hDPSCs, hPDLSCs and NIH-3T3, and then comparative analysis were made between each group to see their respective colony-forming efficiency (CFE) assay and immunofluorescence (IPO13,CK3/12). RESULTS: The efficiency of the four type cells in supporting the LSCs morphology and its cellular differentiation was similar to that of NIH-3T3 fibroblasts as demonstrated by the immunostaining properties analysis, with each group exhibiting a similar strong expression pattern of IPO13, but lacking CK3 and CK12 expression in terms of immunostaining. But hUCMSCs, hDPSCs and hPDLSCs feeder layers were superior in promoting colony formation potential of cells when compared to hUVECs and feeder-cell-free culture. CONCLUSION: hUCMSCs, hDPSCs and hPDLSCs can be a suitable alternative to conventional mouse NIH-3T3 feeder cells, so that risk of zoonotic infection can be diminished.http://www.ijo.cn/en_publish/2017/7/20170701.pdf1027limbal stem cellsfeeder layersumbilical cord mesenchymal stem cellsumbilical vein endothelial cellsdental pulp stem cellsperiodontal ligament stem cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui-Xian Wang Xiao-Wei Gao Bing Ren Yan Cai Wen-Jing Li Yu-Li Yang Yi-Jian Li |
spellingShingle |
Hui-Xian Wang Xiao-Wei Gao Bing Ren Yan Cai Wen-Jing Li Yu-Li Yang Yi-Jian Li Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells International Journal of Ophthalmology 1027 limbal stem cells feeder layers umbilical cord mesenchymal stem cells umbilical vein endothelial cells dental pulp stem cells periodontal ligament stem cells |
author_facet |
Hui-Xian Wang Xiao-Wei Gao Bing Ren Yan Cai Wen-Jing Li Yu-Li Yang Yi-Jian Li |
author_sort |
Hui-Xian Wang |
title |
Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells |
title_short |
Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells |
title_full |
Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells |
title_fullStr |
Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells |
title_full_unstemmed |
Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells |
title_sort |
comparative analysis of different feeder layers with 3t3 fibroblasts for culturing rabbits limbal stem cells |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
International Journal of Ophthalmology |
issn |
2222-3959 2227-4898 |
publishDate |
2017-07-01 |
description |
AIM: To explore the possibility of human umbilical cord mesenchymal stem cells (hUCMSCs), human umbilical vein endothelial cells (hUVECs), human dental pulp stem cells (hDPSCs) and human periodontal ligament stem cells (hPDLSCs) serving as feeder cells in co-culture systems for the cultivation of limbal stem cells.
METHODS: Different feeder layers were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F12 and were treated with mitomycin C. Rabbits limbal stem cells (LSCs) were co-cultured on hUCMSCs, hUVECs, hDPSCs, hPDLSCs and NIH-3T3, and then comparative analysis were made between each group to see their respective colony-forming efficiency (CFE) assay and immunofluorescence (IPO13,CK3/12).
RESULTS: The efficiency of the four type cells in supporting the LSCs morphology and its cellular differentiation was similar to that of NIH-3T3 fibroblasts as demonstrated by the immunostaining properties analysis, with each group exhibiting a similar strong expression pattern of IPO13, but lacking CK3 and CK12 expression in terms of immunostaining. But hUCMSCs, hDPSCs and hPDLSCs feeder layers were superior in promoting colony formation potential of cells when compared to hUVECs and feeder-cell-free culture.
CONCLUSION: hUCMSCs, hDPSCs and hPDLSCs can be a suitable alternative to conventional mouse NIH-3T3 feeder cells, so that risk of zoonotic infection can be diminished. |
topic |
1027 limbal stem cells feeder layers umbilical cord mesenchymal stem cells umbilical vein endothelial cells dental pulp stem cells periodontal ligament stem cells |
url |
http://www.ijo.cn/en_publish/2017/7/20170701.pdf |
work_keys_str_mv |
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