Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae).
Young larvae of the honey bee (Apis mellifera) are totipotent; they can become either queens (reproductives) or workers (largely sterile helpers). DNA methylation has been shown to play an important role in this differentiation. In this study, we examine the contributions of diet and cell size to ca...
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doaj-c3f2225e42cf4fbcbcfdf26c60e1ceca2020-11-25T02:25:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-04-0164e1880810.1371/journal.pone.0018808Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae).Yuan Yuan ShiZachary Y HuangZhi Jiang ZengZi Long WangXiao Bo WuWei Yu YanYoung larvae of the honey bee (Apis mellifera) are totipotent; they can become either queens (reproductives) or workers (largely sterile helpers). DNA methylation has been shown to play an important role in this differentiation. In this study, we examine the contributions of diet and cell size to caste differentiation.We measured the activity and gene expression of one key enzyme involved in methylation, Dnmt3; the rates of methylation in the gene dynactin p62; as well as morphological characteristics of adult bees developed either from larvae fed with worker jelly or royal jelly; and larvae raised in either queen or worker cells. We show that both diet type and cell size contributed to the queen-worker differentiation, and that the two factors affected different methylation sites inside the same gene dynactin p62.We confirm previous findings that Dnmt3 plays a critical role in honey bee caste differentiation. Further, we show for the first time that cell size also plays a role in influencing larval development when diet is kept the same.http://europepmc.org/articles/PMC3082534?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuan Yuan Shi Zachary Y Huang Zhi Jiang Zeng Zi Long Wang Xiao Bo Wu Wei Yu Yan |
spellingShingle |
Yuan Yuan Shi Zachary Y Huang Zhi Jiang Zeng Zi Long Wang Xiao Bo Wu Wei Yu Yan Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae). PLoS ONE |
author_facet |
Yuan Yuan Shi Zachary Y Huang Zhi Jiang Zeng Zi Long Wang Xiao Bo Wu Wei Yu Yan |
author_sort |
Yuan Yuan Shi |
title |
Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae). |
title_short |
Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae). |
title_full |
Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae). |
title_fullStr |
Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae). |
title_full_unstemmed |
Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae). |
title_sort |
diet and cell size both affect queen-worker differentiation through dna methylation in honey bees (apis mellifera, apidae). |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-04-01 |
description |
Young larvae of the honey bee (Apis mellifera) are totipotent; they can become either queens (reproductives) or workers (largely sterile helpers). DNA methylation has been shown to play an important role in this differentiation. In this study, we examine the contributions of diet and cell size to caste differentiation.We measured the activity and gene expression of one key enzyme involved in methylation, Dnmt3; the rates of methylation in the gene dynactin p62; as well as morphological characteristics of adult bees developed either from larvae fed with worker jelly or royal jelly; and larvae raised in either queen or worker cells. We show that both diet type and cell size contributed to the queen-worker differentiation, and that the two factors affected different methylation sites inside the same gene dynactin p62.We confirm previous findings that Dnmt3 plays a critical role in honey bee caste differentiation. Further, we show for the first time that cell size also plays a role in influencing larval development when diet is kept the same. |
url |
http://europepmc.org/articles/PMC3082534?pdf=render |
work_keys_str_mv |
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