ER–Mitochondria Contact Sites Reporters: Strengths and Weaknesses of the Available Approaches
Organelle intercommunication represents a wide area of interest. Over the last few decades, increasing evidence has highlighted the importance of organelle contact sites in many biological processes including Ca<sup>2+</sup> signaling, lipid biosynthesis, apoptosis, and autophagy but als...
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doaj-c40a5a27767e4b8494e0332c5dd0e6562020-11-25T03:33:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-01218157815710.3390/ijms21218157ER–Mitochondria Contact Sites Reporters: Strengths and Weaknesses of the Available ApproachesFlavia Giamogante0Lucia Barazzuol1Marisa Brini2Tito Calì3Department of Biomedical Sciences, University of Padova, 35131 Padova, ItalyDepartment of Biomedical Sciences, University of Padova, 35131 Padova, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyDepartment of Biomedical Sciences, University of Padova, 35131 Padova, ItalyOrganelle intercommunication represents a wide area of interest. Over the last few decades, increasing evidence has highlighted the importance of organelle contact sites in many biological processes including Ca<sup>2+</sup> signaling, lipid biosynthesis, apoptosis, and autophagy but also their involvement in pathological conditions. ER–mitochondria tethering is one of the most investigated inter-organelle communications and it is differently modulated in response to several cellular conditions including, but not limited to, starvation, Endoplasmic Reticulum (ER) stress, and mitochondrial shape modifications. Despite many studies aiming to understand their functions and how they are perturbed under different conditions, approaches to assess organelle proximity are still limited. Indeed, better visualization and characterization of contact sites remain a fascinating challenge. The aim of this review is to summarize strengths and weaknesses of the available methods to detect and quantify contact sites, with a main focus on ER–mitochondria tethering.https://www.mdpi.com/1422-0067/21/21/8157ER–mitochondria tetheringsplit-GFPSPLICSorganelle contact sites |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Flavia Giamogante Lucia Barazzuol Marisa Brini Tito Calì |
spellingShingle |
Flavia Giamogante Lucia Barazzuol Marisa Brini Tito Calì ER–Mitochondria Contact Sites Reporters: Strengths and Weaknesses of the Available Approaches International Journal of Molecular Sciences ER–mitochondria tethering split-GFP SPLICS organelle contact sites |
author_facet |
Flavia Giamogante Lucia Barazzuol Marisa Brini Tito Calì |
author_sort |
Flavia Giamogante |
title |
ER–Mitochondria Contact Sites Reporters: Strengths and Weaknesses of the Available Approaches |
title_short |
ER–Mitochondria Contact Sites Reporters: Strengths and Weaknesses of the Available Approaches |
title_full |
ER–Mitochondria Contact Sites Reporters: Strengths and Weaknesses of the Available Approaches |
title_fullStr |
ER–Mitochondria Contact Sites Reporters: Strengths and Weaknesses of the Available Approaches |
title_full_unstemmed |
ER–Mitochondria Contact Sites Reporters: Strengths and Weaknesses of the Available Approaches |
title_sort |
er–mitochondria contact sites reporters: strengths and weaknesses of the available approaches |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-10-01 |
description |
Organelle intercommunication represents a wide area of interest. Over the last few decades, increasing evidence has highlighted the importance of organelle contact sites in many biological processes including Ca<sup>2+</sup> signaling, lipid biosynthesis, apoptosis, and autophagy but also their involvement in pathological conditions. ER–mitochondria tethering is one of the most investigated inter-organelle communications and it is differently modulated in response to several cellular conditions including, but not limited to, starvation, Endoplasmic Reticulum (ER) stress, and mitochondrial shape modifications. Despite many studies aiming to understand their functions and how they are perturbed under different conditions, approaches to assess organelle proximity are still limited. Indeed, better visualization and characterization of contact sites remain a fascinating challenge. The aim of this review is to summarize strengths and weaknesses of the available methods to detect and quantify contact sites, with a main focus on ER–mitochondria tethering. |
topic |
ER–mitochondria tethering split-GFP SPLICS organelle contact sites |
url |
https://www.mdpi.com/1422-0067/21/21/8157 |
work_keys_str_mv |
AT flaviagiamogante ermitochondriacontactsitesreportersstrengthsandweaknessesoftheavailableapproaches AT luciabarazzuol ermitochondriacontactsitesreportersstrengthsandweaknessesoftheavailableapproaches AT marisabrini ermitochondriacontactsitesreportersstrengthsandweaknessesoftheavailableapproaches AT titocali ermitochondriacontactsitesreportersstrengthsandweaknessesoftheavailableapproaches |
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1724560905200467968 |