IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters
Abstract Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin(IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Pesquisa Odontológica
|
Series: | Brazilian Oral Research |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100209&lng=en&tlng=en |
id |
doaj-c428df3f6b564f56b3b4ca011c44eab8 |
---|---|
record_format |
Article |
spelling |
doaj-c428df3f6b564f56b3b4ca011c44eab82020-11-24T22:08:36ZengSociedade Brasileira de Pesquisa Odontológica Brazilian Oral Research1807-310731010.1590/1807-3107bor-2017.vol31.0012S1806-83242017000100209IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parametersEmine PIRIM GORGUNHulya TOKERErtan Mahir KORKMAZOmer POYRAZAbstract Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin(IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10 gene polymorphisms on the clinical outcomes of non-surgical periodontal therapy and cytokine levels in gingival crevicular fluid(GCF) and serum. Fifty-three patients with GAgP and 50 periodontally healthy individuals were included in this study. Clinical parameters, GCF and blood samples were collected at baseline and at 6-week. Non-surgical periodontal therapy was performed in patients with GAgP. Gene analysis were determined by PCR-RFLP(polymerase chain reaction-restriction fragment length polymorphism) and cytokine levels were determined by enzyme-linked immunosorbent assay(ELISA).GAgP patients showed significant improvement on clinical parameters after periodontal therapy(p<0.05). In the GAgP group, IL-6 GG genotype and G allele frequency were higher than in the control group. GCF IL-6 level was also significantly lower at 6-week in the GAgP group. Higher GCF IL-10 levelswere observed in patients carrying the IL-6 GG genotype than in those carrying the GC+CC genotype at baseline. In conclusion, IL-6(-174) and IL-10(-597) gene polymorphisms were found to be associated with GAgP and genotype distribution did not affect the outcome of non-surgical periodontal therapy, while patients with IL-6(-174) GG genotype had higher levels of GCF IL-10 levels.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100209&lng=en&tlng=enInterleukin-6Interleukin-10Polymorphism, GeneticAggressive Periodontitis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emine PIRIM GORGUN Hulya TOKER Ertan Mahir KORKMAZ Omer POYRAZ |
spellingShingle |
Emine PIRIM GORGUN Hulya TOKER Ertan Mahir KORKMAZ Omer POYRAZ IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters Brazilian Oral Research Interleukin-6 Interleukin-10 Polymorphism, Genetic Aggressive Periodontitis |
author_facet |
Emine PIRIM GORGUN Hulya TOKER Ertan Mahir KORKMAZ Omer POYRAZ |
author_sort |
Emine PIRIM GORGUN |
title |
IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
title_short |
IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
title_full |
IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
title_fullStr |
IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
title_full_unstemmed |
IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
title_sort |
il-6 and il-10 gene polymorphisms in patients with aggressive periodontitis: effects on gcf, serum and clinic parameters |
publisher |
Sociedade Brasileira de Pesquisa Odontológica |
series |
Brazilian Oral Research |
issn |
1807-3107 |
description |
Abstract Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin(IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10 gene polymorphisms on the clinical outcomes of non-surgical periodontal therapy and cytokine levels in gingival crevicular fluid(GCF) and serum. Fifty-three patients with GAgP and 50 periodontally healthy individuals were included in this study. Clinical parameters, GCF and blood samples were collected at baseline and at 6-week. Non-surgical periodontal therapy was performed in patients with GAgP. Gene analysis were determined by PCR-RFLP(polymerase chain reaction-restriction fragment length polymorphism) and cytokine levels were determined by enzyme-linked immunosorbent assay(ELISA).GAgP patients showed significant improvement on clinical parameters after periodontal therapy(p<0.05). In the GAgP group, IL-6 GG genotype and G allele frequency were higher than in the control group. GCF IL-6 level was also significantly lower at 6-week in the GAgP group. Higher GCF IL-10 levelswere observed in patients carrying the IL-6 GG genotype than in those carrying the GC+CC genotype at baseline. In conclusion, IL-6(-174) and IL-10(-597) gene polymorphisms were found to be associated with GAgP and genotype distribution did not affect the outcome of non-surgical periodontal therapy, while patients with IL-6(-174) GG genotype had higher levels of GCF IL-10 levels. |
topic |
Interleukin-6 Interleukin-10 Polymorphism, Genetic Aggressive Periodontitis |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100209&lng=en&tlng=en |
work_keys_str_mv |
AT eminepirimgorgun il6andil10genepolymorphismsinpatientswithaggressiveperiodontitiseffectsongcfserumandclinicparameters AT hulyatoker il6andil10genepolymorphismsinpatientswithaggressiveperiodontitiseffectsongcfserumandclinicparameters AT ertanmahirkorkmaz il6andil10genepolymorphismsinpatientswithaggressiveperiodontitiseffectsongcfserumandclinicparameters AT omerpoyraz il6andil10genepolymorphismsinpatientswithaggressiveperiodontitiseffectsongcfserumandclinicparameters |
_version_ |
1725815745486520320 |