A Novel Cellulase Produced by a Newly Isolated Trichoderma virens
Screening and obtaining a novel high activity cellulase and its producing microbe strain is the most important and essential way to improve the utilization of crop straw. In this paper, we devoted our efforts to isolating a novel microbe strain which could produce high activity cellulase. A novel st...
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doaj-c49df2779073410b83c9f34fb94975452020-11-24T22:22:33ZengMDPI AGBioengineering2306-53542016-04-01321310.3390/bioengineering3020013bioengineering3020013A Novel Cellulase Produced by a Newly Isolated Trichoderma virensRong Zeng0Xiao-Yan Yin1Tao Ruan2Qiao Hu3Ya-Li Hou4Zhen-Yu Zuo5Hao Huang6Zhong-Hua Yang7College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, ChinaScreening and obtaining a novel high activity cellulase and its producing microbe strain is the most important and essential way to improve the utilization of crop straw. In this paper, we devoted our efforts to isolating a novel microbe strain which could produce high activity cellulase. A novel strain Trichoderma virens ZY-01 was isolated from a cropland where straw is rich and decomposed, by using the soil dilution plate method with cellulose and Congo red. The strain has been licensed with a patent numbered ZL 201210295819.6. The cellulase activity in the cultivation broth could reach up to 7.4 IU/mL at a non-optimized fermentation condition with the newly isolated T. virens ZY-01. The cellulase was separated and purified from the T. virens culture broth through (NH4)2SO4 fractional precipitation, anion-exchange chromatography and gel filtration chromatography. With the separation process, the CMC specific activity increased from 0.88 IU/mg to 31.5 IU/mg with 35.8 purification fold and 47.04% yield. Furthermore, the enzymatic properties of the cellulase were investigated. The optimum temperature and pH is 50 °C and pH 5.0 and it has good thermal stability. Zn2+, Ca2+ and Mn2+ could remarkably promote the enzyme activity. Conversely, Cu2+ and Co2+ could inhibit the enzymatic activity. This work provides a new highly efficient T. virens strain for cellulase production and shows good prospects in practical application.http://www.mdpi.com/2306-5354/3/2/13Trichoderma virenscellulasestrain isolationstrawbiomass |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rong Zeng Xiao-Yan Yin Tao Ruan Qiao Hu Ya-Li Hou Zhen-Yu Zuo Hao Huang Zhong-Hua Yang |
spellingShingle |
Rong Zeng Xiao-Yan Yin Tao Ruan Qiao Hu Ya-Li Hou Zhen-Yu Zuo Hao Huang Zhong-Hua Yang A Novel Cellulase Produced by a Newly Isolated Trichoderma virens Bioengineering Trichoderma virens cellulase strain isolation straw biomass |
author_facet |
Rong Zeng Xiao-Yan Yin Tao Ruan Qiao Hu Ya-Li Hou Zhen-Yu Zuo Hao Huang Zhong-Hua Yang |
author_sort |
Rong Zeng |
title |
A Novel Cellulase Produced by a Newly Isolated Trichoderma virens |
title_short |
A Novel Cellulase Produced by a Newly Isolated Trichoderma virens |
title_full |
A Novel Cellulase Produced by a Newly Isolated Trichoderma virens |
title_fullStr |
A Novel Cellulase Produced by a Newly Isolated Trichoderma virens |
title_full_unstemmed |
A Novel Cellulase Produced by a Newly Isolated Trichoderma virens |
title_sort |
novel cellulase produced by a newly isolated trichoderma virens |
publisher |
MDPI AG |
series |
Bioengineering |
issn |
2306-5354 |
publishDate |
2016-04-01 |
description |
Screening and obtaining a novel high activity cellulase and its producing microbe strain is the most important and essential way to improve the utilization of crop straw. In this paper, we devoted our efforts to isolating a novel microbe strain which could produce high activity cellulase. A novel strain Trichoderma virens ZY-01 was isolated from a cropland where straw is rich and decomposed, by using the soil dilution plate method with cellulose and Congo red. The strain has been licensed with a patent numbered ZL 201210295819.6. The cellulase activity in the cultivation broth could reach up to 7.4 IU/mL at a non-optimized fermentation condition with the newly isolated T. virens ZY-01. The cellulase was separated and purified from the T. virens culture broth through (NH4)2SO4 fractional precipitation, anion-exchange chromatography and gel filtration chromatography. With the separation process, the CMC specific activity increased from 0.88 IU/mg to 31.5 IU/mg with 35.8 purification fold and 47.04% yield. Furthermore, the enzymatic properties of the cellulase were investigated. The optimum temperature and pH is 50 °C and pH 5.0 and it has good thermal stability. Zn2+, Ca2+ and Mn2+ could remarkably promote the enzyme activity. Conversely, Cu2+ and Co2+ could inhibit the enzymatic activity. This work provides a new highly efficient T. virens strain for cellulase production and shows good prospects in practical application. |
topic |
Trichoderma virens cellulase strain isolation straw biomass |
url |
http://www.mdpi.com/2306-5354/3/2/13 |
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