<i>Autophagy-Related 2</i> Regulates Chlorophyll Degradation under Abiotic Stress Conditions in <i>Arabidopsis</i>

• Main Authors: Zhuanzhuan Jiang, Li Zhu, Qiuyu Wang, Xin Hou
• Format: Article
• Language: English
• Published: MDPI AG 2020-06-01
• Series: International Journal of Molecular Sciences
• Subjects: chloroplast degradation; autophagy; ATG2; high light intensity; high temperature; chloroplast vesiculation
• Online Access: https://www.mdpi.com/1422-0067/21/12/4515
• ISSN: 1661-6596; 1422-0067

Chloroplasts are extraordinary organelles for photosynthesis and nutrient storage in plants. During leaf senescence or under stress conditions, damaged chloroplasts are degraded and provide nutrients for developing organs. Autophagy is a high-throughput degradation pathway for intracellular material turnover in eukaryotes. Along with chloroplast degradation, chlorophyll, an important component of the photosynthetic machine, is also degraded. However, the chlorophyll degradation pathways under high light intensity and high temperature stress are not well known. Here, we identified and characterized a novel <i>Arabidopsis</i> mutant, <i>sl2 </i>(<i>seedling lethal 2</i>), showing defective chloroplast development and accelerated chlorophyll degradation. Map-based cloning combined with high-throughput sequencing analysis revealed that a 118.6 kb deletion region was associated with the phenotype of the mutant. Complementary experiments confirmed that the loss of function of <i>ATG2</i> was responsible for accelerating chlorophyll degradation in <i>sl2</i> mutants. Furthermore, we analyzed chlorophyll degradation under abiotic stress conditions and found that both chloroplast vesiculation and autophagy take part in chlorophyll degradation under high light intensity and high temperature stress. These results enhanced our understanding of chlorophyll degradation under high light intensity and high temperature stress.