Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihype...
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doaj-c4e15902795548709357d00d26c98c072020-11-24T20:46:04ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882015-01-01201510.1155/2015/272457272457Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful ConditionsKhalida Mokhtari0Eva E. Rufino-Palomares1Amalia Pérez-Jiménez2Fernando J. Reyes-Zurita3Celeny Figuera4Leticia García-Salguero5Pedro P. Medina6Juan Peragón7José A. Lupiáñez8Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, SpainDepartment of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, SpainDepartment of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, SpainDepartment of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, SpainDepartment of Biochemistry, Faculty of Sciences, Simón Bolívar University of Caracas, Caracas 1080, VenezuelaDepartment of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, SpainDepartment of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, SpainDepartment of Experimental Biology, Biochemistry Section, Faculty of Experimental Biology, University of Jaén, 23071 Jaén, SpainDepartment of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, SpainMaslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.http://dx.doi.org/10.1155/2015/272457 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Khalida Mokhtari Eva E. Rufino-Palomares Amalia Pérez-Jiménez Fernando J. Reyes-Zurita Celeny Figuera Leticia García-Salguero Pedro P. Medina Juan Peragón José A. Lupiáñez |
spellingShingle |
Khalida Mokhtari Eva E. Rufino-Palomares Amalia Pérez-Jiménez Fernando J. Reyes-Zurita Celeny Figuera Leticia García-Salguero Pedro P. Medina Juan Peragón José A. Lupiáñez Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions Evidence-Based Complementary and Alternative Medicine |
author_facet |
Khalida Mokhtari Eva E. Rufino-Palomares Amalia Pérez-Jiménez Fernando J. Reyes-Zurita Celeny Figuera Leticia García-Salguero Pedro P. Medina Juan Peragón José A. Lupiáñez |
author_sort |
Khalida Mokhtari |
title |
Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions |
title_short |
Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions |
title_full |
Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions |
title_fullStr |
Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions |
title_full_unstemmed |
Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions |
title_sort |
maslinic acid, a triterpene from olive, affects the antioxidant and mitochondrial status of b16f10 melanoma cells grown under stressful conditions |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2015-01-01 |
description |
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained. |
url |
http://dx.doi.org/10.1155/2015/272457 |
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